{"id":11656,"date":"2025-07-31T09:00:30","date_gmt":"2025-07-31T00:00:30","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/07\/31\/11656\/"},"modified":"2025-07-31T09:00:30","modified_gmt":"2025-07-31T00:00:30","slug":"%ec%a0%9c%ed%95%9c%ed%9a%a8%ec%86%8c%eb%a5%bc-%ec%9d%b4%ec%9a%a9%ed%95%9c-dna%ec%a0%88%eb%8b%a8%ec%a0%84%ea%b8%b0-%ec%98%81%eb%8f%99%eb%b2%95-%eb%b0%8f-pcr%ec%97%90-%ec%9d%98%ed%95%9c-dna","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/07\/31\/11656\/","title":{"rendered":"\uc81c\ud55c\ud6a8\uc18c\ub97c \uc774\uc6a9\ud55c DNA\uc808\ub2e8&\uc804\uae30 \uc601\ub3d9\ubc95 \ubc0f PCR\uc5d0 \uc758\ud55c DNA\ubd84\ub9ac \ubc0f \ud655\uc778"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\uc81c\ubaa9<\/p>\n

2. \uc2e4\ud5d8\ubaa9\uc801<\/p>\n

3. \uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d<\/p>\n

4. \uc2e4\ud5d8\uc6d0\ub9ac
\n4.1 PCR
\n4.2 \uc804\uae30\uc601\ub3d9
\n4.3 competent cell
\n4.4 \uc720\uc804\uc790 \uc7ac\uc870\ud569
\n4.5 \ud615\uc9c8\uc804\ud658
\n4.6 Ethidium Bromide <\/p>\n

5. \uc2e4\ud5d8 \ubc29\ubc95<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc2e4\ud5d8\uc81c\ubaa9: \uc81c\ud55c\ud6a8\uc18c\ub97c \uc774\uc6a9\ud55c DNA\uc808\ub2e8& \uc804\uae30 \uc601\ub3d9\ubc95 \ubc0f PCR\uc5d0 \uc758\ud55c DNA\ubd84\ub9ac \ubc0f \ud655\uc778<\/p>\n

2. \uc2e4\ud5d8\ubaa9\uc801: \ubbf8\uc0dd\ubb3c\uc5d0\uc11c plasmid DNA\ub97c \ubd84\ub9ac\ud558\uace0, PCR\uc744 \uc774\uc6a9\ud558\uc5ec DNA\ub97c \uc99d\ud3ed\ud558\uace0 \uc81c\ud55c\ud6a8\uc18c\ub97c \uc774\uc6a9\ud558\uc5ec DNA\ub97c \uc808\ub2e8\ud55c \ud6c4 Mini gel unit\ub97c \uc774\uc6a9\ud558\uc5ec \uc804\uae30\uc601\ub3d9\ubc95\uc5d0 \uc758\ud574 DNA\ub97c \ubd84\ub9ac\ud558\uace0 \ud655\uc778\ud55c\ub2e4. <\/p>\n

3.\uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d
\nGradient PCR, Mini Gel Electrophoresis Units(Mupid21), agarose gel, Microcentrifuge tube, \uc99d\ub958\uc218, \uc81c\ud55c\ud6a8\uc18c, buffer\uc6a9\uc561, PCR premix tube, \uc99d\ub958\uc218, primer, DMSO, \uc8fc\ud615 DNA, size marker, circular vector plasmid, loading dye, circular recombinent plasmid<\/p>\n

4. \uc2e4\ud5d8\uc6d0\ub9ac<\/p>\n

4.1 PCR
\n1. PCR\uc758 \uc6d0\ub9ac
\n PCR\ubc95\uc758 \uae30\ubcf8 \uc6d0\ub9ac\ub294 \ub2e4\uc74c\uacfc \uac19\ub2e4.
\n \uccab \ub2e8\uacc4\ub294 \uc774\uc911\uc1c4 DNA\ub97c \ub2e8\uc77c\uc1c4 DNA\ub85c \uc5f4\ubcc0\uc131(denaturation)\uc2dc\ud0a8\ub2e4. \ub450 \ubc88\uc9f8 \ub2e8\uacc4\ub294 \uc62c\ub9ac\uace0 \ub274\ud074\ub808\uc624\ud2f0\ub4dc\ub97c \ub2e8\uc77c\uc1c4 DNA\uc5d0 \uacb0\ud569(annealing)\uc2dc\ud0a4\uace0 \uc138 \ubc88\uc9f8 \ub2e8\uacc4\ub294 \uacb0\ud569\ud55c \uc62c\ub9ac\uace0\ub274\ud074\ub808\uc624\ud2f0\ub4dc\ub97c \uc2dc\ubc1c\uccb4\ub85c \ud558\uc5ec DNA \ud3f4\ub9ac\uba38\ub77c\uc81c\uc5d0 \uc758\ud574 DNA\ub97c \ud569\uc131(Extension)\ud558\uc5ec \uc774\uc0c1\uc758 \uacfc\uc815\uc744 \uc77c\uc815\ud55c \uc2f8\uc774\ud074\ub3d9\uc548 \ubc18\ubcf5\ud55c\ub2e4.
\n \uc774\ub860\uc801\uc73c\ub85c \ub9e4 PCR \uc8fc\uae30\ub9c8\ub2e4 \uc99d\ud3ed\ub418\ub294 DNA\ub294 2\ubc30\uc529 \ub298\uc5b4\ub098\uc57c \ud558\ub098 (n \uc2f8\uc774\ud074 \ud6c4\ub294 2n\uac1c) \uc5ec\ub7ec \uac00\uc9c0 \uc774\uc720\uc5d0\uc11c \uc77c\uc815 \uc8fc\uae30\uac00 \uc9c0\ub098\uba74 \uc99d\ud3ed\ub418\ub294 DNA\uc758 \uc591\uc774 \uc548\uc815\uae30(plateau)\uc5d0 \ub3c4\ub2ec\ud558\uac8c \ub41c\ub2e4. PCR\ub85c \uc99d\ud3ed\ub41c \ubc34\ub4dc\ub97c \uc5d0\ud2f0\ub514\uc6c0 \ube0c\ub85c\ub9c8\uc774\ub4dc(EtBr)\ub85c \uc5fc\uc0c9\ub41c \uc544\uac00\ub85c\uc2a4 \uc824(Agarose gel)\uc5d0\uc11c \uad00\ucc30\ud558\ub824\uba74 \uc57d 50ng\uc758 DNA\uac00 \ud544\uc694\ud55c\ub370 50ng\uc740 300bp\uae38\uc774 DNA\ub85c \ub530\uc9c0\uba74 \uc57d 1011\uce74\ud53c\uac00 \ub41c\ub2e4. \ub530\ub77c\uc11c \ub2e8\uc77c copy\uc758 300bp DNA\ub97c \uc99d\ud3ed \ud6c4 \uc804\uae30\uc601\ub3d9\ud558\uc5ec \ubcfc \uc218 \uc788\uc73c\ub824\uba74 \uc57d 37 \uc2f8\uc774\ud074\uc774\uba74 (237=1011)\ub41c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\uc81c\ubaa9 2. \uc2e4\ud5d8\ubaa9\uc801 3. \uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d 4. \uc2e4\ud5d8\uc6d0\ub9ac 4.1 PCR 4.2 \uc804\uae30\uc601\ub3d9 4.3 competent cell 4.4 \uc720\uc804\uc790 \uc7ac\uc870\ud569 4.5 \ud615\uc9c8\uc804\ud658 4.6 Ethidium Bromide 5. \uc2e4\ud5d8 \ubc29\ubc95 \ubcf8\ubb38\ub0b4\uc6a9 1. \uc2e4\ud5d8\uc81c\ubaa9: \uc81c\ud55c\ud6a8\uc18c\ub97c \uc774\uc6a9\ud55c DNA\uc808\ub2e8& \uc804\uae30 \uc601\ub3d9\ubc95 \ubc0f PCR\uc5d0 \uc758\ud55c DNA\ubd84\ub9ac \ubc0f \ud655\uc778 2. \uc2e4\ud5d8\ubaa9\uc801: \ubbf8\uc0dd\ubb3c\uc5d0\uc11c plasmid DNA\ub97c \ubd84\ub9ac\ud558\uace0, PCR\uc744 \uc774\uc6a9\ud558\uc5ec DNA\ub97c \uc99d\ud3ed\ud558\uace0 \uc81c\ud55c\ud6a8\uc18c\ub97c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[17110,9088,2633,17109,17111,9087],"class_list":{"0":"post-11656","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-uncategorized","7":"tag-dna","9":"tag-pcr","10":"tag-17109","11":"tag-17111","12":"tag-9087"},"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/11656","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=11656"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/11656\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=11656"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=11656"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=11656"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}