{"id":11670,"date":"2025-07-31T09:00:45","date_gmt":"2025-07-31T00:00:45","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/07\/31\/11670\/"},"modified":"2025-07-31T09:00:45","modified_gmt":"2025-07-31T00:00:45","slug":"e-coli_transformation_%ed%98%95%ec%a7%88%ec%a0%84%ed%99%98-%ec%8b%a4%ed%97%98%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/07\/31\/11670\/","title":{"rendered":"E.coli_transformation_\ud615\uc9c8\uc804\ud658 \uc2e4\ud5d8\ubcf4\uace0\uc11c"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Transformation\uc758 \uc6d0\ub9ac
\n(1) \ud615\uc9c8\uc804\ud658\uc758 \uc2dc\uc791
\n(2) \ud615\uc9c8\uc804\ud658\uc758 \uba54\ucee4\ub2c8\uc998
\n(3) E.coli\uc758 \ud615\uc9c8 \uc804\ud658(transformation)<\/p>\n

2. \uc2e4\ud5d8\uc7ac\ub8cc<\/p>\n

3. \uc2e4\ud5d8\ubc29\ubc95
\n(1) competent cell \uc81c\uc870
\n(2) transformation \ubc29\ubc95<\/p>\n

4. \uc2e4\ud5d8\uacb0\uacfc<\/p>\n

5. \uc2e4\ud5d8\uace0\ucc30 <\/p>\n

6. \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\ud615\uc9c8\uc804\ud658\uc740 \uc678\ubd80\ub85c\ubd80\ud130 \uc8fc\uc5b4\uc9c4 DNA\uc5d0 \uc758\ud558\uc5ec \uc0dd\ubb3c\uc758 \uc720\uc804\uc801\uc778 \uc131\uc9c8\uc774 \ubcc0\ud558\ub294 \uc77c\uc744 \ub9d0\ud55c\ub2e4. \ud615\uc9c8\uc804\ud658\uc740 1928\ub144 \uc601\uad6d\uc758 F.\uadf8\ub9ac\ud53c\uc2a4\uac00 \ud3d0\ub834\uc30d\uad6c\uade0(\u80ba\u708e\u96d9\u7403\u83cc)\uc744 \uc774\uc6a9\ud558\uc5ec \uc2e4\uc2dc\ud55c \uc2e4\ud5d8\uc774 \uacc4\uae30\uac00 \ub418\uc5b4 \ubc1c\uacac\ub418\uc5c8\ub2e4. \ub2e4\ub2f9\ub958\ub85c \uc774\ub8e8\uc5b4\uc9c4 \ub450\uaebc\uc6b4 \ub9c9, \uc989 \ud611\ub9c9(\u83a2\u819c)\uc744 \uac00\uc9c4 S\ud615\uade0\uc73c\ub85c\ubd80\ud130 DNA\ub97c \ucd94\ucd9c\ud558\uc5ec \uc774\uac83\uc744 \ub2e4\ub2f9\ub958\uc131 \ud611\ub9c9\uc744 \uac00\uc9c0\uc9c0 \uc54a\uc740 R\ud615\uade0\uc5d0 \uc791\uc6a9\uc2dc\ud0a4\uba74 R\ud615\uade0\uc758 \uc77c\ubd80\uac00 S\ud615\uade0\uc73c\ub85c \ubcc0\ud55c\ub2e4. \uc774\uac83\uc740 S\ud615 \uc138\uade0\uc758 DAN\uac00 R\ud615 \uc138\uade0 \uc18d\uc5d0 \ub4e4\uc5b4\uac00\uc11c \uc720\uc804\uc790 \ubc1c\ud604\uc744 \ud558\uae30 \ub54c\ubb38\uc774\ub2e4. \uc804\ud658\uc744 \uc77c\uc73c\ud0a4\ub294 \ub370 \ud544\uc694\ud55c DNA\uc758 \ucd5c\uc18c\ub7c9\uc740 \uc57d 10\ub9cc\ubd84\uc758 1 mol\uc774\uba70, DNA\uc758 single strand\ub97c \uc8fc\ub294 \uac83\ubcf4\ub2e4\ub3c4 double strand\ub97c \uc8fc\ub294 \ucabd\uc774 \uc804\ud658\uc744 \uc77c\uc73c\ud0a4\uae30 \uc27d\ub2e4. \ud615\uc9c8\uc804\ud658 \uc2e4\ud5d8\uc5d0 \uc758\ud558\uc5ec \uc720\uc804\uc790\uc758 \ubcf8\uccb4\uac00 DNA\ub77c\ub294 \uac83\uc774 \ubc1d\ud600\uc84c\ub2e4. \uadf8\ub7ec\ub098 \uace0\ub4f1\uc0dd\ubb3c\uc5d0 \uc788\uc5b4\uc11c\ub3c4 \uc138\uade0\ub958\uc5d0\uc11c \ubcfc \uc218 \uc788\ub294 \uac83 \uac19\uc740 \ud615\uc9c8\uc804\ud658\uc774 \uc77c\uc5b4\ub098\ub294\uc9c0 \uc5b4\ub5a4\uc9c0\ub294 \uc544\uc9c1 \ubc1d\ud600\uc9c0\uc9c0 \uc54a\uc558\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Transformation\uc758 \uc6d0\ub9ac (1) \ud615\uc9c8\uc804\ud658\uc758 \uc2dc\uc791 (2) \ud615\uc9c8\uc804\ud658\uc758 \uba54\ucee4\ub2c8\uc998 (3) E.coli\uc758 \ud615\uc9c8 \uc804\ud658(transformation) 2. \uc2e4\ud5d8\uc7ac\ub8cc 3. \uc2e4\ud5d8\ubc29\ubc95 (1) competent cell \uc81c\uc870 (2) transformation \ubc29\ubc95 4. \uc2e4\ud5d8\uacb0\uacfc 5. \uc2e4\ud5d8\uace0\ucc30 6. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \ud615\uc9c8\uc804\ud658\uc740 \uc678\ubd80\ub85c\ubd80\ud130 \uc8fc\uc5b4\uc9c4 DNA\uc5d0 \uc758\ud558\uc5ec \uc0dd\ubb3c\uc758 \uc720\uc804\uc801\uc778 \uc131\uc9c8\uc774 \ubcc0\ud558\ub294 \uc77c\uc744 \ub9d0\ud55c\ub2e4. \ud615\uc9c8\uc804\ud658\uc740 1928\ub144 \uc601\uad6d\uc758 F.\uadf8\ub9ac\ud53c\uc2a4\uac00 \ud3d0\ub834\uc30d\uad6c\uade0(\u80ba\u708e\u96d9\u7403\u83cc)\uc744 \uc774\uc6a9\ud558\uc5ec \uc2e4\uc2dc\ud55c \uc2e4\ud5d8\uc774 \uacc4\uae30\uac00 \ub418\uc5b4 \ubc1c\uacac\ub418\uc5c8\ub2e4. \ub2e4\ub2f9\ub958\ub85c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[17138,17137,12090,9093,2635,17139],"class_list":["post-11670","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-competent-cell","tag-e-coli","tag-transformation","tag-9093","tag-2635","tag-17139"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/11670","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=11670"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/11670\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=11670"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=11670"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=11670"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}