{"id":13529,"date":"2025-08-19T09:00:03","date_gmt":"2025-08-19T00:00:03","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13529\/"},"modified":"2025-08-19T09:00:03","modified_gmt":"2025-08-19T00:00:03","slug":"%ec%83%9d%eb%ac%bc%ea%b3%b5%ec%a0%95%ec%8b%a4%ed%97%98-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c_gene-expression-analysis-by-rt-pcr","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13529\/","title":{"rendered":"\uc0dd\ubb3c\uacf5\uc815\uc2e4\ud5d8 \uacb0\uacfc\ubcf4\uace0\uc11c_Gene Expression Analysis by RT-PCR"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/12\/data29450615-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/12\/data29450615-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>1.\t\uc2e4\ud5d8\ubaa9\uc801<br \/>\n2.\t\uc2e4\ud5d8\ubc29\ubc95<br \/>\n3.\t\uc2e4\ud5d8\uacb0\uacfc<br \/>\n4.\t\uace0\ucc30<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p>-\uc2e4\ud5d8 \ubaa9\uc801<br \/>\nreverse transcription PCR\uc744 \uc5bb\uc5b4\uc9c4 cDNA sample\uc744 real time PCR\uc744 \ud1b5\ud574 \uc99d\ud3ed \uc2dc\ud0a8\ub2e4. \uc774\ub54c \ub4e4\uc5b4 \uac00\ub294 reagent\ub4e4 \ud2b9\ud788 cancer cell\uacfc ADSC cell\uc758 gene\uc5d0 \uac01\uac01 \ub2e4\ub978 primer\ub97c \ucc98\ub9ac\ud568\uc5d0 \ub530\ub77c Housekeeping gene\uc778 GAPDH\uc5d0 \ube44\ud574 Thy-1 gene\uc758 \ubc1c\ud604\uc758 \uc99d\uac10\uc744 \uc54c\uc544\ubcf4\uba70, \uc774\ub4e4\uc758 \uc6d0\ub9ac \ubc0f real time PCR\ubd84\uc11d\uc5d0 \ub530\ub978 \ub098\ud0c0\ub09c \uacb0\uacfc \uac12\ub4e4\uc744 \uc815\uc81c\ud558\uba70 \ubd84\uc11d\ud55c\ub2e4.<\/p>\n<p>-\uc2e4\ud5d8 \ubc29\ubc95<br \/>\n1. \uc704\uc758 \uc2dc\uc57d\ub4e4\uc744 10% overage\ud558\uac8c \ub123\uc5b4 pipetting loss\ub97c \ubc29\uc9c0\ud55c\ub2e4. cDNA\ub294 5~50ng\uc744\uc0ac\uc6a9\ud55c\ub2e4.<br \/>\n2. briefly vortexing\ud55c\ub2e4\uc74c briefly centrifuge\ud55c\ub2e4. PCR mixture\uac00 \uc798 \uc11e\uc600\ub294\uc9c0 \ud655\uc778\ud55c\ub2e4.<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/29450615\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc2e4\ud5d8\ubc29\ubc95 3. \uc2e4\ud5d8\uacb0\uacfc 4. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 -\uc2e4\ud5d8 \ubaa9\uc801 reverse transcription PCR\uc744 \uc5bb\uc5b4\uc9c4 cDNA sample\uc744 real time PCR\uc744 \ud1b5\ud574 \uc99d\ud3ed \uc2dc\ud0a8\ub2e4. \uc774\ub54c \ub4e4\uc5b4 \uac00\ub294 reagent\ub4e4 \ud2b9\ud788 cancer cell\uacfc ADSC cell\uc758 gene\uc5d0 \uac01\uac01 \ub2e4\ub978 primer\ub97c \ucc98\ub9ac\ud568\uc5d0 \ub530\ub77c Housekeeping gene\uc778 GAPDH\uc5d0 \ube44\ud574 Thy-1 gene\uc758 \ubc1c\ud604\uc758 \uc99d\uac10\uc744 \uc54c\uc544\ubcf4\uba70, \uc774\ub4e4\uc758 \uc6d0\ub9ac \ubc0f real time PCR\ubd84\uc11d\uc5d0 \ub530\ub978 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18733,1849,18734,18728],"class_list":["post-13529","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-18733","tag-1849","tag-18734","tag-18728"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=13529"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13529\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=13529"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=13529"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=13529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}