{"id":13530,"date":"2025-08-19T09:00:04","date_gmt":"2025-08-19T00:00:04","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13530\/"},"modified":"2025-08-19T09:00:04","modified_gmt":"2025-08-19T00:00:04","slug":"%ec%83%9d%eb%ac%bc%ea%b3%b5%ec%a0%95%ec%8b%a4%ed%97%98-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c_mammalian-suspension-cell-culture","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13530\/","title":{"rendered":"\uc0dd\ubb3c\uacf5\uc815\uc2e4\ud5d8 \uacb0\uacfc\ubcf4\uace0\uc11c_Mammalian Suspension Cell Culture"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\t\uc2e4\ud5d8\ubaa9\uc801<\/p>\n

2.\t\uc2e4\ud5d8\ubc29\ubc95
\n1)\t<suspension cell preparation>
\n2)\t<Counting cells using Hemocytometer><\/p>\n

3.\t\uc2e4\ud5d8\uacb0\uacfc \ubc0f \ud574\uc11d
\n1)\t<hemocytometer \uce21\uc815 \uacb0\uacfc>
\n2)\t<Countess II \uce21\uc815 \uacb0\uacfc><\/p>\n

4.\t\uace0\ucc30<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

-\uc2e4\ud5d8 \ubaa9\uc801
\nCHO cell\uc758 \ud2b9\uc9d5\uc744 \uc54c\uc544\ubcf4\uace0, \uc9c1\uc811 suspension culture\ub41c cell\uc758 viability\ub97c Hemocytometer\uc640 Countess II\ub97c \uc774\uc6a9\ud574 \uce21\uc815\ud574\ubcf8\ub2e4.
\n-\uc2e4\ud5d8 \ubc29\ubc95
\n
\n1. CHO cell\uc774 \ub2f4\uaca8 \uc788\ub358 plate\uc758 \uc624\ub798\ub41c \ubc30\uc9c0\ub97c \ubc84\ub9b0 \ub4a4 FBS washing\ud55c\ub2e4.
\n2. FBS washing\ud55c\ub2e4.
\n3. cell counting\uc744 \uc704\ud574 EP tube\uc5d0 \uc62e\uaca8 \ub2f4\ub294\ub2e4.
\n(\uc704 \uacfc\uc815\uc740 \uc870\uad50\ub2d8\uc774 \uc900\ube44 \ud574 \uc8fc\uc168\ub2e4.)
\n
\n1. EP tube\uc5d0 \uc900\ube44\ub41c CHO cell\uc744 trypan blue\ub85c 1\/4 \ud76c\uc11d\ud558\uba70 (70\/70)ug\uc73c\ub85c 1\/2\ud76c\uc11d\ud55c \ud6c4 \ud76c\uc11d\uc561 \uc5d0\uc11c \ud55c\ubc88 \ub354 70ul\uc744 \uc5bb\uc5b4\uc900 \ub4a4 \uc5ec\uae30\uc5d0 trypan blue 70ul\uc744 \ub123\uc5b4\uc918 1\/4\ub85c \ud76c\uc11d \ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc2e4\ud5d8\ubc29\ubc95 1) <suspension cell preparation> 2) <Counting cells using Hemocytometer> 3. \uc2e4\ud5d8\uacb0\uacfc \ubc0f \ud574\uc11d 1) <hemocytometer \uce21\uc815 \uacb0\uacfc> 2) <Countess II \uce21\uc815 \uacb0\uacfc> 4. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 -\uc2e4\ud5d8 \ubaa9\uc801 CHO cell\uc758 \ud2b9\uc9d5\uc744 \uc54c\uc544\ubcf4\uace0, \uc9c1\uc811 suspension culture\ub41c cell\uc758 viability\ub97c Hemocytometer\uc640 Countess II\ub97c \uc774\uc6a9\ud574 \uce21\uc815\ud574\ubcf8\ub2e4. -\uc2e4\ud5d8 \ubc29\ubc95 1. CHO cell\uc774 \ub2f4\uaca8 \uc788\ub358 plate\uc758 \uc624\ub798\ub41c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18733,1849,18734,18728],"class_list":["post-13530","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-18733","tag-1849","tag-18734","tag-18728"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13530","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=13530"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13530\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=13530"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=13530"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=13530"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}