{"id":13531,"date":"2025-08-19T09:00:04","date_gmt":"2025-08-19T00:00:04","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13531\/"},"modified":"2025-08-19T09:00:04","modified_gmt":"2025-08-19T00:00:04","slug":"%ec%83%9d%eb%ac%bc%ea%b3%b5%ec%a0%95%ec%8b%a4%ed%97%98-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c_p1-transduction","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13531\/","title":{"rendered":"\uc0dd\ubb3c\uacf5\uc815\uc2e4\ud5d8 \uacb0\uacfc\ubcf4\uace0\uc11c_P1 Transduction"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/12\/data29450619-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/12\/data29450619-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>1.\t\uc2e4\ud5d8\ubaa9\uc801<br \/>\n2.\t\uc2e4\ud5d8\ubc29\ubc95<br \/>\n3.\t\uc2e4\ud5d8\uacb0\uacfc<br \/>\n4.\t\uace0\ucc30<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p><\uc2e4\ud5d8 \ubaa9\uc801><br \/>\n\ubc15\ud14c\ub9ac\uc544\uc758 \ud615\uc9c8\uc804\ud658 \ubc29\ubc95 \uc911 \ud558\ub098\uc778 Transduction\uc744 phage\ub97c \ud1b5\ud574 \uc9c4\ud589\ud558\uba70, Transduction\uc5ec\ubd80\ub97c \ud2b9\uc815 \uc870\uac74\uc758 \ubc30\uc9c0\uc5d0\uc11c \ud655\uc778 \ud55c\ub2e4.<br \/>\n<\uc2e4\ud5d8 \ubc29\ubc95><br \/>\n1. cell, (cell+phage)\ub77c\uace0 \uc801\ud78ce-tube\uc5d0 resuspension\ud55c bacterial\uc744 10ul\uc529 \ub123\uc5b4\uc900\ub2e4.<br \/>\n2.\uac80\uc740\uc0c9 e-tube\uc5d0 T1-Phage\uac00 \uc788\uc73c\ubbc0\ub85c \uc774\ub97c 10ul \ub530\uc11c cell + phage e-tube\uc5d0 \ubd84\uc8fc\ud55c\ub2e4.<br \/>\n3. p1 salt\ub97c \ub123\uc5b4\uc900 \ub4a4 \ud30c\uc9c0\uac00 \ucda9\ubd84\ud788 transduction \ud558\ub3c4\ub85d 30\ubd84\uac04 37\u2103\uc5d0\uc11c incubation\uc744 \uc9c4\ud589\ud55c\ub2e4.<br \/>\n4. 1ml\uc758 LB EGTA\ub97c \ubd84\uc8fc\ud55c \ub4a4 37\u2103\uc5d0\uc11c 30\ubd84\uac04 incubation\ud574\uc900\ub2e4.<br \/>\n5. \uac01\uac01\uc758 e-tube\ub97c 2\ubd84\uac04 13000g\uc5d0\uc11c \uc6d0\uc2ec\ubd84\ub9ac \ud55c\ub2e4.<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/29450619\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc2e4\ud5d8\ubc29\ubc95 3. \uc2e4\ud5d8\uacb0\uacfc 4. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 \ubc15\ud14c\ub9ac\uc544\uc758 \ud615\uc9c8\uc804\ud658 \ubc29\ubc95 \uc911 \ud558\ub098\uc778 Transduction\uc744 phage\ub97c \ud1b5\ud574 \uc9c4\ud589\ud558\uba70, Transduction\uc5ec\ubd80\ub97c \ud2b9\uc815 \uc870\uac74\uc758 \ubc30\uc9c0\uc5d0\uc11c \ud655\uc778 \ud55c\ub2e4. 1. cell, (cell+phage)\ub77c\uace0 \uc801\ud78ce-tube\uc5d0 resuspension\ud55c bacterial\uc744 10ul\uc529 \ub123\uc5b4\uc900\ub2e4. 2.\uac80\uc740\uc0c9 e-tube\uc5d0 T1-Phage\uac00 \uc788\uc73c\ubbc0\ub85c \uc774\ub97c 10ul \ub530\uc11c cell + phage e-tube\uc5d0 \ubd84\uc8fc\ud55c\ub2e4. 3. p1 salt\ub97c \ub123\uc5b4\uc900 \ub4a4 \ud30c\uc9c0\uac00 \ucda9\ubd84\ud788 transduction \ud558\ub3c4\ub85d 30\ubd84\uac04 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18733,1849,18734,18728],"class_list":["post-13531","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-18733","tag-1849","tag-18734","tag-18728"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13531","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=13531"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13531\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=13531"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=13531"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=13531"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}