{"id":13532,"date":"2025-08-19T09:00:05","date_gmt":"2025-08-19T00:00:05","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13532\/"},"modified":"2025-08-19T09:00:05","modified_gmt":"2025-08-19T00:00:05","slug":"%ec%83%9d%eb%ac%bc%ea%b3%b5%ec%a0%95%ec%8b%a4%ed%97%98-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c_rna-extraction-and-quantification","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13532\/","title":{"rendered":"\uc0dd\ubb3c\uacf5\uc815\uc2e4\ud5d8 \uacb0\uacfc\ubcf4\uace0\uc11c_RNA Extraction and Quantification"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/12\/data29450621-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/12\/data29450621-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>1.\t\uc2e4\ud5d8\ubaa9\uc801<br \/>\n2.\t\uc2e4\ud5d8\ubc29\ubc95<br \/>\n3.\t\uc2e4\ud5d8\uacb0\uacfc<br \/>\n4.\t\uace0\ucc30<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p>-\uc2e4\ud5d8 \ubaa9\uc801<br \/>\nTrizol\uc744 \uc774\uc6a9\ud574 RNA \ucd94\ucd9c \ubc0f \uc815\uc81c\ud558\ub294 \ubc29\ubc95\uc744 \uc775\ud788\uba70 \ucd94\ucd9c\ub41c \uc0b0\ubb3c\uc758 Quality\uc640 Quantity\ub97c \ud655\uc778\ud574 \ubcf8\ub2e4.<br \/>\n-\uc2e4\ud5d8 \ubc29\ubc95<br \/>\nRNA extraction<br \/>\n<Phase Separation><br \/>\n1. 6-well plate\uc5d0 (ADSC, Cancer cell)\uc744 \uc900\ube44\ud55c\ub2e4\uc74c well\uc5d0 \ub2f4\uae34 media\ub97c 1ml \ud30c\uc774\ud3ab\uc744 \uc81c\uac70\ud55c\ub2e4.<br \/>\n2. PBS 1ml\uc744 \uac01 well\ub9c8\ub2e4 \ub123\uc5b4\uc900 \ub4a4 plate\ub97c \uac00\ubccd\uac8c \ud754\ub4e4\uc5b4 \uc8fc\uba70 washing\ud55c\ub2e4. \uc774\ub54c washing\ud558\ub294 \uc774\uc720\ub294 Media\uc5d0 \ub0a8\uc544\uc788\ub294 \ub2e8\ubc31\uc9c8\ub4e4\uc744 \uc81c\uac70\ud574 \ucd94\ud6c4\uc758 \uc2dc\uc57d\ub4e4\uc774 \ub2e8\ubc31\uc9c8\ub4e4\uacfc \ubc18\uc751\ud558\uc9c0 \uc54a\ub3c4\ub85d \ud558\ub294 \uac83\uc774\ub2e4.<br \/>\n3. PBS\ub97c \uc81c\uac70\ud574 \uc8fc\uace0 TRIzol 0.8ml\uc744 \ub123\uc5b4\uc900 \ub2e4\uc74c \uc0c1\uc628\uc5d0\uc11c 5\ubd84\uac04 incubation\ud55c\ub2e4.<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/29450621\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc2e4\ud5d8\ubc29\ubc95 3. \uc2e4\ud5d8\uacb0\uacfc 4. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 -\uc2e4\ud5d8 \ubaa9\uc801 Trizol\uc744 \uc774\uc6a9\ud574 RNA \ucd94\ucd9c \ubc0f \uc815\uc81c\ud558\ub294 \ubc29\ubc95\uc744 \uc775\ud788\uba70 \ucd94\ucd9c\ub41c \uc0b0\ubb3c\uc758 Quality\uc640 Quantity\ub97c \ud655\uc778\ud574 \ubcf8\ub2e4. -\uc2e4\ud5d8 \ubc29\ubc95 RNA extraction 1. 6-well plate\uc5d0 (ADSC, Cancer cell)\uc744 \uc900\ube44\ud55c\ub2e4\uc74c well\uc5d0 \ub2f4\uae34 media\ub97c 1ml \ud30c\uc774\ud3ab\uc744 \uc81c\uac70\ud55c\ub2e4. 2. PBS 1ml\uc744 \uac01 well\ub9c8\ub2e4 \ub123\uc5b4\uc900 \ub4a4 plate\ub97c \uac00\ubccd\uac8c \ud754\ub4e4\uc5b4 \uc8fc\uba70 washing\ud55c\ub2e4. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18733,1849,18734,18728],"class_list":["post-13532","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-18733","tag-1849","tag-18734","tag-18728"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13532","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=13532"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13532\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=13532"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=13532"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=13532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}