{"id":13533,"date":"2025-08-19T09:00:05","date_gmt":"2025-08-19T00:00:05","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13533\/"},"modified":"2025-08-19T09:00:05","modified_gmt":"2025-08-19T00:00:05","slug":"%ec%83%9d%eb%ac%bc%ea%b3%b5%ec%a0%95%ec%8b%a4%ed%97%98-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c_spore-sterilization-for-food-processing","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/08\/19\/13533\/","title":{"rendered":"\uc0dd\ubb3c\uacf5\uc815\uc2e4\ud5d8 \uacb0\uacfc\ubcf4\uace0\uc11c_Spore sterilization for food processing"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\t\uc2e4\ud5d8\ubaa9\uc801<\/p>\n

2.\t\uc2e4\ud5d8\ubc29\ubc95
\n1)\tFiltration
\n2)\tIncubation\/heat shock
\n3)\tDilution
\n4)\tSpreading<\/p>\n

3.\t\uc2e4\ud5d8\uacb0\uacfc<\/p>\n

4.\t\uace0\ucc30<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

<\uc2e4\ud5d8 \ubaa9\uc801>
\n\ub2e4\uc591\ud55c \ubc29\ubc95\uc73c\ub85c bacterial\uc744 \uba78\uade0\ud558\uace0 \uba78\uade0\ub41c \uc815\ub3c4\ub97c \ud655\uc778\ud55c\ub2e4.
\n<\uc2e4\ud5d8 \ubc29\ubc95>
\n-Filtration
\n1. syringe\ub294 \uc8fc\uc0ac \ubc14\ub298\uc744 \uc81c\uac70\ud574 \uc8fc\uace0 0.2um, 1.2um membrane filter\ub97c \uacb0\ud569\ud55c\ub2e4.
\n2. \uc8fc\uc0ac\uae30 \ub05d \ubd80\ubd84\uacfc \ube48 conical tube\ub97c \uc190\uc73c\ub85c \uc798 \uace0\uc815\ud558\uace0 3ml\uc758 spore, vegetative sample\uc744 vortexing\uc744 \uc9c4\ud589\ud55c \ub2e4\uc74c syringe\uc5d0 \ub123\uc5b4\uc900 \ub2e4\uc74c filtration\uc744 \uc9c4\ud589\ud55c\ub2e4. (\uc774\ub54c membrane\uc774 \ucc22\uc5b4\uc9c8 \uc218 \uc788\uae30 \ub54c\ubb38\uc5d0 plunger\ub97c \uc81c\uac70\ud55c \ub2e4\uc74c membrane\uc744 \uacb0\ud569 \ud574 \uc900\ub2e4.)
\n-Incubation\/heat shock
\n1. vegetative, spore cell\uc744 500ul\uc529 RT, 60\ub77c\uace0 \uc801\ud600 \uc788\ub294 ep tube\uc5d0 500ul\uc529 \ubd84\uc8fc \ud574 \uc900\ub2e4.
\n2. RT\uc5d0\uc11c incubation 5\ubd84, 60\ub3c4\uc5d0\uc11c heat shock\uc744 5\ubd84\uac04 \uc9c4\ud589 \ud6c4 \ubcf4\uad00\ud574 \uc900\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc2e4\ud5d8\ubc29\ubc95 1) Filtration 2) Incubation\/heat shock 3) Dilution 4) Spreading 3. \uc2e4\ud5d8\uacb0\uacfc 4. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 \ub2e4\uc591\ud55c \ubc29\ubc95\uc73c\ub85c bacterial\uc744 \uba78\uade0\ud558\uace0 \uba78\uade0\ub41c \uc815\ub3c4\ub97c \ud655\uc778\ud55c\ub2e4. -Filtration 1. syringe\ub294 \uc8fc\uc0ac \ubc14\ub298\uc744 \uc81c\uac70\ud574 \uc8fc\uace0 0.2um, 1.2um membrane filter\ub97c \uacb0\ud569\ud55c\ub2e4. 2. \uc8fc\uc0ac\uae30 \ub05d \ubd80\ubd84\uacfc \ube48 conical tube\ub97c \uc190\uc73c\ub85c \uc798 \uace0\uc815\ud558\uace0 3ml\uc758 spore, vegetative sample\uc744 vortexing\uc744 \uc9c4\ud589\ud55c \ub2e4\uc74c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18733,1849,18734,18728],"class_list":["post-13533","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-18733","tag-1849","tag-18734","tag-18728"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13533","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=13533"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/13533\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=13533"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=13533"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=13533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}