{"id":14911,"date":"2025-09-01T09:01:25","date_gmt":"2025-09-01T00:01:25","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/01\/14911\/"},"modified":"2025-09-01T09:01:25","modified_gmt":"2025-09-01T00:01:25","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%981-a-%ec%9e%90%eb%a3%8c-1-sampling-spreading-cell-counting","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/01\/14911\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(1) A \uc790\ub8cc) 1. Sampling, Spreading, Cell Counting"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c
\n2. \uc2e4\ud5d8 \ub3c4\uad6c
\n3. \uc774\ub860
\n4. \uc2e4\ud5d8 \ubaa9\ud45c
\n5. \uc2e4\ud5d8 \ubc29\ubc95
\n6. \uacb0\uacfc
\n7. \uace0\ucc30
\n8. \ucd9c\ucc98<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

2. \uc2e4\ud5d8 \ub3c4\uad6c
\n– Sample : \ud654\uc7a5\uc2e4 \ubcc0\uae30\uc5d0\uc11c \ucde8\ud568, \ud759
\n– \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301 (1000 \u03bcL, 200 \u03bcL), tube (10 mL, 50 mL), tube rack, vortex mixer, clean bench, \ud398\ud2b8\ub9ac \uc811\uc2dc, \ub3c4\ub9d0\ubd09, \uc54c\ucf54\uc62c\ub7a8\ud504, \ub77c\ud14d\uc2a4 \uc7a5\uac11
\n– \uc2dc\uc57d : PBS, LB \ubc30\uc9c0, \uc54c\ucf54\uc62c<\/p>\n

3. \uc774\ub860
\n\u2460 Sampling
\n \uac80\uc0ac, \uce21\uc815, \ubd84\uc11d \ub4f1\uc744 \uc704\ud574 \ub300\uc0c1\uc774 \ub418\ub294 \ubb3c\uc9c8\uc5d0\uc11c \uadf8 \uc77c\ubd80\ub97c \ucc44\ucde8\ud558\uc5ec \uc870\uc0ac\ud558\ub294 \uac83\uc73c\ub85c, \uc2e4\ud5d8\uc758 \ud544\uc694\uc131\uc5d0 \ub9de\ub294 \uc801\uc808\ud55c sample\uc744 \uad6c\ud558\uc5ec\uc57c \ud55c\ub2e4.
\n\u2461 Serial dilution
\n \ubbf8\ub9ac \uacb0\uc815\ud55c \ube44\uc728\uc5d0 \ub530\ub77c \uc21c\ucc28\uc801\uc73c\ub85c \ud76c\uc11d\ud558\ub294 \ubc29\ubc95\uc774\ub2e4. \ubbf8\uc0dd\ubb3c\uc758 \uc218\ub97c \uce21\uc815\ud560 \ub54c \uc2dc\ub8cc\ub97c 10\ubc30 \uc529 \ub2e8\uacc4\uc801\uc73c\ub85c \ud76c\uc11d\ud558\ub294 \uac83\uc774\uba70, \ud76c\uc11d\ud55c \uc2dc\ub8cc\ub97c \uc801\uc808\ud55c \ubc30\uc9c0\uc5d0 \ubc30\uc591\ud558\uc5ec colony\ub97c \ud615\uc131\ud558\ub294 \ubbf8\uc0dd\ubb3c\uc758 \uc218\ub97c \uce21\uc815\ud55c\ub2e4. \uc774\ud6c4 \ud76c\uc11d \ubc30\uc218\ub97c \uacf1\ud558\uc5ec \uc2dc\ub8cc\uc5d0 \uc788\ub294 \ubbf8\uc0dd\ubb3c\uc758 \uc218\ub97c \uad6c\ud558\ub294 \uac83\uc774\ub2e4. \ud76c\uc11d\ud55c \uc2dc\ub8cc \uc18d \uade0\uc758 \ub18d\ub3c4\ub97c \ub0ae\ucd94\uc5b4 \ubc30\uc591\ud55c \ud6c4 colony\uac00 30~300\uac1c\uac00 \ub418\uba74 \uc218\ub97c \uc138\uc5b4 CFU\ub97c \ud1b5\ud574 \ud76c\uc11d \uc804 \uc2dc\ub8cc\uc758 \uc138\uade0 \uc218\ub97c \uacc4\uc0b0\ud560 \uc218 \uc788\ub2e4.
\n\u2462 CFU(Colony-Forming Unit)
\nCFU = \ud3c9\uade0 \uc9d1\ub77d\uc218 \u00d7 \ud76c\uc11d\ubc30\uc728 \u00d7 1\/(\uc0ac\uc6a9\ud55c \uc2dc\ub8cc\ub7c9(mL)) (CFU\/mL or CFU\/g)
\n \ubbf8\uc0dd\ubb3c\ud559\uc5d0\uc11c, \ub208\uc5d0 \ubcf4\uc774\ub294 \ubc15\ud14c\ub9ac\uc544\ub098 \uade0\ub958\uc758 \uc22b\uc790\ub97c \uce21\uc815\ud558\ub294 \uac83\uc774\ub2e4. \uc561\uccb4\uc5d0\uc11c\ub294 CFU\/mL, \uace0\uccb4\uc5d0\uc11c\ub294 CFU\/g\uc758 \ub2e8\uc704\ub97c \uc0ac\uc6a9\ud55c\ub2e4. \uc9d1\ub77d\uc758 \uc218\uac00 \uc140 \uc218 \uc5c6\uc744 \uc815\ub3c4\ub85c \ub9ce\uc744 \ub54c\ub294 TNTC(Too Numerous To Count)\ub85c \ud45c\uc2dc\ud558\uba70 \uc9d1\ub77d\uc758 \uc218\uac00 \uce21\uc815 \uac00\ub2a5 \ud560 \ub54c\ub294 TFTC(Too Few To Count)\ub85c \ud45c\uc2dc\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c 2. \uc2e4\ud5d8 \ub3c4\uad6c 3. \uc774\ub860 4. \uc2e4\ud5d8 \ubaa9\ud45c 5. \uc2e4\ud5d8 \ubc29\ubc95 6. \uacb0\uacfc 7. \uace0\ucc30 8. \ucd9c\ucc98 \ubcf8\ubb38\ub0b4\uc6a9 2. \uc2e4\ud5d8 \ub3c4\uad6c – Sample : \ud654\uc7a5\uc2e4 \ubcc0\uae30\uc5d0\uc11c \ucde8\ud568, \ud759 – \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301 (1000 \u03bcL, 200 \u03bcL), tube (10 mL, 50 mL), tube rack, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7354,20025,7362,20022,20024,20026,20023,20020,20021,8319],"class_list":["post-14911","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cell-counting","tag-cfu","tag-colony-formation","tag-sampling","tag-serial-dilution","tag-spread-plate-method","tag-spreading","tag-20020","tag-20021","tag-8319"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/14911","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=14911"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/14911\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=14911"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=14911"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=14911"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}