{"id":15200,"date":"2025-09-04T09:00:56","date_gmt":"2025-09-04T00:00:56","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15200\/"},"modified":"2025-09-04T09:00:56","modified_gmt":"2025-09-04T00:00:56","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%981-a-%ec%9e%90%eb%a3%8c-3-growth-curve","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15200\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(1) A \uc790\ub8cc) 3. Growth curve"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c
\n2. \uc2e4\ud5d8 \ub3c4\uad6c
\n3. \uc774\ub860
\n4. \uc2e4\ud5d8 \ubaa9\ud45c
\n5. \uc2e4\ud5d8 \ubc29\ubc95
\n6. \uacb0\uacfc
\n7. \uace0\ucc30
\n8. \ucd9c\ucc98<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

2. \uc2e4\ud5d8 \ub3c4\uad6c
\n– Sample : \ud759\uc744 \uc7ac\ub8cc\ub85c streaking \uacfc\uc815\uc744 \ud1b5\ud574 \uc5bb\uc740 single colony \uc911, \ub69c\ub837\ud55c \uac83\uc744 \ucc44\ucde8\ud558\uc5ec \uc0ac\uc6a9
\n– \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301 (1000 \u03bcL, 200 \u03bcL), tube (10 mL, 50 mL), tube rack, clean bench, plate, \uc54c\ucf54\uc62c\ub7a8\ud504, \ub77c\ud14d\uc2a4 \uc7a5\uac11, \uc0bc\uac01 \ud50c\ub77c\uc2a4\ud06c, shaking incubator, cuvette, UV\/Vis Spectrophotometer, \ud638\uc77c
\n– \uc2dc\uc57d : LB \ubc30\uc9c0, \uc561\uccb4 \ubc30\uc9c0, DW, \uc5d0\ud0c4\uc62c<\/p>\n

3. \uc774\ub860
\n\u2460 Growth curve(\uc0dd\uc7a5 \uace1\uc120)
\n\uc0dd\uccb4\uc758 \uc2dc\uac04\uc5d0 \ub300\ud55c \uc0dd\uc7a5 \uc0c1\ud0dc\ub97c \uadf8 \uac1c\uccb4\uc758 \ud06c\uae30, \uc911\ub7c9\uc758 \uc99d\uac00 \ub610\ub294 \uc138\ud3ec\uc218\uc758 \uc99d\uac00 \ub4f1\uc744 \uac00\uc9c0\uace0 \ud45c\ud604\ud55c \uace1\uc120\uc774\ub2e4. \ud2b9\uc9d5\uc801\uc778 \uc99d\uc2dd\uc0c1\uc744 \ub300\ubcc4\ud558\uc5ec, \uc720\ub3c4\uae30, \ub300\uc218\uae30, \uc815\uc9c0\uae30, \uc0ac\uba78\uae30\ub85c \ub098\ub25c\ub2e4. \ucc98\uc74c\uc5d0 \uade0\uc740 \ubd84\uc5f4\uc744 \uc77c\uc73c\ud0a4\uc9c0 \uc54a\uace0 \uc138\ub300 \uc2dc\uac04\uc774 \uae34 \uc720\ub3c4\uae30\ub97c \uc9c0\ub09c\ub2e4. \uc774\uc5b4\uc11c \uc810\ucc28 \ubd84\uc5f4\uc774 \ube68\ub77c\uc9c0\uace0, Log\uae30\uac00 \ub41c\ub2e4. \ub9c8\uc9c0\ub9c9\uc5d0 \ub2e4\uc2dc \uade0\uc758 \ubd84\uc5f4\uc740 \uc644\ub9cc\ud558\uac8c \ub418\uc5b4 \uade0\uc218\uc758 \uc99d\uac10\uc740 \uc5c6\uc5b4\uc9c0\uace0, \uc815\uc0c1\uae30\ub85c \ub41c\ub2e4.
\n\u2461 Generation(doubling) time (\uc138\ub300\uc2dc\uac04)
\n\uc774\ubd84\ubc95\uc73c\ub85c \uc0dd\uc7a5\ud558\ub294 \ub2e8\uc138\ud3ec \uc0dd\ubb3c\uc5d0\uc11c, \uad70\uc9d1\uc5d0\uc11c \ub2e8\uc138\ud3ec \uc0dd\ubb3c\uc758 \uc218\uac00 \ub450 \ubc30\ub85c \ub298\uc5b4\ub098\ub294 \ub370 \uac78\ub9ac\ub294 \uc2dc\uac04\uc744 \uc758\ubbf8\ud55c\ub2e4. \uadf8\ub7f0 \uc758\ubbf8\uc5d0\uc11c \ubc30\uc99d \uc2dc\uac04(doubling time)\uc774\ub77c\uace0\ub3c4 \ubd80\ub978\ub2e4. Generation time\uc740 \ubc30\uc591\uc628\ub3c4, \ubc30\uc9c0 \ub0b4 \uc601\uc591\ubd84 \ubc0f \uc0b0\uc18c \ub18d\ub3c4, pH \ub4f1 \ubc30\uc591 \uc870\uac74\uc5d0 \ub530\ub77c \ub2ec\ub77c\uc9c4\ub2e4. \ubcf4\ud1b5 generation time\uc740 \uc8fc\uc5b4\uc9c4 \ubbf8\uc0dd\ubb3c\uc774 \uac00\uc7a5 \ube68\ub9ac \uc790\ub784 \uc218 \uc788\ub294 \uc870\uac74\uc5d0\uc11c \uce21\uc815\ub41c \uac12\uc744 \uc0ac\uc6a9\ud558\ub294\ub370, \uc774 \uac12\uc740 \uade0\uc758 \uc885\ub958\uc5d0 \ub530\ub77c \uc218\uc2ed\ubd84\uc5d0\uc11c \uc218\uc2ed\uc2dc\uac04\uae4c\uc9c0 \ub2e4\uc591\ud558\uac8c \ubd84\ud3ec\ub41c\ub2e4. \ub300\uc7a5\uade0\uc758 \uacbd\uc6b0\uc5d0\ub294 generation time\uc774 20\ubd84\uc5d0 \ubd88\uacfc\ud558\uc9c0\ub9cc, \uacb0\ud575\uade0\uc758 \uacbd\uc6b0\ub294 \uc57d 18~54 \uc2dc\uac04 \uc815\ub3c4\uac00 \uc18c\uc694\ub41c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c 2. \uc2e4\ud5d8 \ub3c4\uad6c 3. \uc774\ub860 4. \uc2e4\ud5d8 \ubaa9\ud45c 5. \uc2e4\ud5d8 \ubc29\ubc95 6. \uacb0\uacfc 7. \uace0\ucc30 8. \ucd9c\ucc98 \ubcf8\ubb38\ub0b4\uc6a9 2. \uc2e4\ud5d8 \ub3c4\uad6c – Sample : \ud759\uc744 \uc7ac\ub8cc\ub85c streaking \uacfc\uc815\uc744 \ud1b5\ud574 \uc5bb\uc740 single colony \uc911, \ub69c\ub837\ud55c \uac83\uc744 \ucc44\ucde8\ud558\uc5ec \uc0ac\uc6a9 – \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301 (1000 \u03bcL, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[20248,20251,7351,20020,20021,4992,20249,20250,12307,8319],"class_list":["post-15200","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-growth-curve","tag-growth-rate","tag-7351","tag-20020","tag-20021","tag-4992","tag-20249","tag-20250","tag-12307","tag-8319"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15200","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=15200"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15200\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=15200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=15200"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=15200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}