{"id":15205,"date":"2025-09-04T09:01:02","date_gmt":"2025-09-04T00:01:02","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15205\/"},"modified":"2025-09-04T09:01:02","modified_gmt":"2025-09-04T00:01:02","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%981-a-%ec%9e%90%eb%a3%8c-4-plasmid-perp-gel-electrophoresis","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15205\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(1) A \uc790\ub8cc) 4. Plasmid perp & Gel Electrophoresis"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c
\n2. \uc2e4\ud5d8 \ub3c4\uad6c
\n3. \uc774\ub860
\n4. \uc2e4\ud5d8 \ubaa9\ud45c
\n5. \uc2e4\ud5d8 \ubc29\ubc95
\n6. \uacb0\uacfc
\n7. \uace0\ucc30
\n8. \ucd9c\ucc98<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

2. \uc2e4\ud5d8 \ub3c4\uad6c
\n– \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301 (1000 \u03bcL, 200 \u03bcL), tube (10 mL, 50 mL), tube rack, \ub77c\ud14d\uc2a4 \uc7a5\uac11, microwave, UV lamp, nano drop, \uc804\uae30\uc601\ub3d9\uc7a5\uce58, shaking incubator, vortex mixer, centrifuge, \uc2a4\ud2f0\ub85c\ud3fc \ubc15\uc2a4, \uc0bc\uac01\ud50c\ub77c\uc2a4\ud06c
\n– \uc2dc\uc57d : E.coli \ubc30\uc591\uc561, agarose powder, TAE buffer, 6X loading dye, isopropanol, 70% EtOH, gel electrophoresis buffer, EtBr, DW, P1, P2, P3 solution, ice<\/p>\n

3. \uc774\ub860
\n\u2460 Plasmid DNA
\n\uc138\uade0\uc758 \uc138\ud3ec \ub0b4\uc5d0 \uc5fc\uc0c9\uccb4\uc640\ub294 \ubcc4\uac1c\ub85c \uc874\uc7ac\ud558\uba74\uc11c \ub3c5\uc790\uc801\uc73c\ub85c \uc99d\uc2dd\ud560 \uc218 \uc788\ub294 DNA\uc774\ub2e4. \uace0\ub9ac \ubaa8\uc591\uc744 \ub760\uace0 \uc788\uc73c\uba70 \uc138\uade0\uc758 \uc0dd\uc874\uc5d0 \ud544\uc218\uc801\uc778 \uc720\uc804\uc790\ub294 \uc544\ub2c8\ub2e4. \uc720\uc804\uacf5\ud559\uc5d0\uc11c\ub294 \uc138\uade0 \ub0b4 \ud50c\ub77c\uc2a4\ubbf8\ub4dc\ub97c \uc138\ud3ec \ubc16\uc73c\ub85c \ube7c\ub0b4\uace0 \uc81c\ud55c\ud6a8\uc18c\ub85c \ub04a\uc740 \ub4a4, \ud544\uc694\ub85c \ud558\ub294 \uc720\uc804\uc790\ub97c \uc0bd\uc785\ud558\uc5ec \uc774\ub97c \ub2e4\uc2dc \uc138\uade0\uc5d0 \ub123\uc5b4 \ubc30\uc591\ud558\ub294 \uc720\uc804\uc790\uc7ac\uc870\ud569 \uae30\uc220\uc744 \uc0ac\uc6a9\ud55c\ub2e4.
\n\u2461 Plasmid DNA\ub97c \ucd94\ucd9c\ud558\ub294 \ubc29\ubc95
\n\u2160. \ud06c\uae30 \ucc28\uc774\ub97c \uc774\uc6a9\ud55c plasmid \ucd94\ucd9c \ubc0f \uc815\uc81c \ubc29\ubc95
\n\uc720\uc804\uccb4 DNA\uac00 \uc808\ub2e8\ub418\uc9c0 \uc54a\ub3c4\ub85d \ub9e4\uc6b0 \uc870\uc2ec\ud788 \uc218\ud589\ud558\uc5ec\uc57c \ud55c\ub2e4. \uba3c\uc800, EDTA, \ub77c\uc774\uc18c\uc790\uc784 \ubc0f 25% \uc790\ub2f9(sucrose) \uc6a9\uc561\uc744 \ucc98\ub9ac\ud558\uc5ec \uc138\ud3ec\ubcbd\uc774 \ubd80\ubd84\uc801\uc73c\ub85c \ud30c\uad34\ub41c \uc6d0\ud615\uc9c8\uccb4\ub97c \ud615\uc131\ud55c \ud6c4, \ube44\uc774\uc628\uc131 \uacc4\uba74\ud65c\uc131\uc81c\uc778 Triton X-100\uc744 \ucca8\uac00\ud558\uc5ec \uc138\ud3ec\ub97c \uc6a9\ud574\uc2dc\ud0a8\ub2e4. \uc774\ud6c4, \uc6d0\uc2ec\ubd84\ub9ac\ub97c \ud1b5\ud574 \uc0c1\uce35\uc561\uc5d0 \uc788\ub294 \ud50c\ub77c\uc2a4\ubbf8\ub4dc\ub97c \ucd94\ucd9c\ud558\uac8c \ub41c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c 2. \uc2e4\ud5d8 \ub3c4\uad6c 3. \uc774\ub860 4. \uc2e4\ud5d8 \ubaa9\ud45c 5. \uc2e4\ud5d8 \ubc29\ubc95 6. \uacb0\uacfc 7. \uace0\ucc30 8. \ucd9c\ucc98 \ubcf8\ubb38\ub0b4\uc6a9 2. \uc2e4\ud5d8 \ub3c4\uad6c – \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301 (1000 \u03bcL, 200 \u03bcL), tube (10 mL, 50 mL), tube rack, \ub77c\ud14d\uc2a4 \uc7a5\uac11, microwave, UV lamp, nano drop, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[3284,20262,16630,10165,20261,20020,20021,20263,20264,8319],"class_list":["post-15205","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-agarose-gel","tag-etbr","tag-gel-electrophoresis","tag-plasmid-dna","tag-plasmid-perp","tag-20020","tag-20021","tag-20263","tag-20264","tag-8319"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15205","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=15205"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15205\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=15205"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=15205"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=15205"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}