{"id":15213,"date":"2025-09-04T09:01:11","date_gmt":"2025-09-04T00:01:11","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15213\/"},"modified":"2025-09-04T09:01:11","modified_gmt":"2025-09-04T00:01:11","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%981-a-%ec%9e%90%eb%a3%8c-5-restriction-enzyme-cutting","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15213\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(1) A \uc790\ub8cc) 5. Restriction Enzyme Cutting"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c
\n2. \uc2e4\ud5d8 \ub3c4\uad6c
\n3. \uc774\ub860
\n4. \uc2e4\ud5d8 \ubaa9\ud45c
\n5. \uc2e4\ud5d8 \ubc29\ubc95
\n6. \uacb0\uacfc
\n7. \uace0\ucc30
\n8. \ucd9c\ucc98<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

2. \uc2e4\ud5d8 \ub3c4\uad6c
\n– \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301, EP tube , tube rack, \ub77c\ud14d\uc2a4 \uc7a5\uac11, microwave, UV lamp, \uc804\uae30\uc601\ub3d9\uc7a5\uce58, shaking incubator, vortex mixer, centrifuge, \uc2a4\ud2f0\ub85c\ud3fc \ubc15\uc2a4, \uc0bc\uac01\ud50c\ub77c\uc2a4\ud06c
\n– \uc2dc\uc57d : Plasma DNA, Not \u2160, Hind \u2162, 10X fast cut buffer, DNA marker, DW, agarose powder, TAE buffer, 6X loading dye, gel electrophoresis buffer, EtBr, ice<\/p>\n

3. \uc774\ub860
\n\u2460 Restriction enzyme
\n\uc138\uade0\uc774 \ubc14\uc774\ub7ec\uc2a4 \ub4f1 \uc678\ubd80\uc5d0\uc11c \uc628 DNA\ub97c \uc808\ub2e8\ud558\uc5ec \ubc30\uc81c\uc2dc\ud0a4\uae30 \uc704\ud574\uc11c \uac00\uc9c0\uace0 \uc788\ub294 \uc77c\uc885\uc758 \uc790\uae30\ubc29\uc5b4\ud6a8\uc18c\uc774\ub2e4. DNA\uc758 \uc5fc\uae30 \uc11c\uc5f4\uc758 \ud2b9\uc815 \ubd80\ubd84\ub9cc\uc744 \uac00\uc218\ubd84\ud574\ud558\ub294 \uc131\uc9c8\uc744 \uac00\uc9c4 \uac83\uc73c\ub85c, \uc720\uc804\uacf5\ud559\uc5d0\uc11c \uc720\uc6a9\ud55c \uc218\ub2e8\uc73c\ub85c \uc774\uc6a9\ub420 \uc218 \uc788\ub2e4. \uc81c\ud55c\ud6a8\uc18c\ub294 \uc774\ub4e4\uc758 \uc870\uc131\uacfc \ud544\uc694\ub85c \ud558\ub294 \ud6a8\uc18c \ubcf4\uc870\uc778\uc790, \uc774\ub4e4\uc774 \uc791\uc6a9\ud558\ub294 \uc11c\uc5f4\uc5d0 \ub530\ub77c \ud06c\uac8c 4\uac1c\uc758 \uadf8\ub8f9\uc73c\ub85c \ub098\ub20c \uc218 \uc788\ub2e4. \ubaa8\ub4e0 \uc81c\ud55c\ud6a8\uc18c\ub294 DNA \ub0b4\ubd80\uc5d0 \uc874\uc7ac\ud558\ub294 \uc9e7\uc740 \uc11c\uc5f4\uc744 \uc778\uc9c0\ud558\uc5ec \uc791\uc6a9\ud55c\ub2e4.
\nType \u2160\uc758 \uacbd\uc6b0 \uc81c\ud55c\ud6a8\uc18c\uc640 \uba54\ud2f8\ud654\ud6a8\uc18c\uac00 \ubb49\uccd0\uc838 \uc788\uace0, \uc778\uc2dd\uc790\ub9ac\uc640 \uc81c\ud55c\uc790\ub9ac\uac00 \uc11c\ub85c \ub2e4\ub974\ub2e4. \uc5fc\uae30\uc11c\uc5f4\uc740 \ud2b9\uc774\uc801\uc73c\ub85c \uc778\uc2dd\ud558\uc9c0\ub9cc, \uc778\uc2dd\uc790\ub9ac\uc5d0\uc11c \uc57d 1000\uac1c \uc5fc\uae30 \uc815\ub3c4 \ub5a8\uc5b4\uc9c4 \uacf3\uc5d0\uc11c \ube44\ud2b9\uc774\uc801\uc73c\ub85c DNA\ub97c \uc790\ub978\ub2e4. \ub610\ud55c, \ud65c\uc131\uc5d0 ATP\ub098 S-\uc544\ub370\ub178\uc2e4\uba54\ud2f0\uc624\ub2cc, Mg2+ \ub4f1\uc744 \ud544\uc694\ub85c \ud55c\ub2e4. DNA \uc808\ub2e8 \uc704\uce58\uc758 \ud2b9\uc774\uc131\uc774 \uc5c6\uae30 \ub54c\ubb38\uc5d0 \uc2e4\ud5d8 \ubaa9\uc801\uc73c\ub85c\ub294 \uac70\uc758 \uc0ac\uc6a9\ud558\uc9c0 \uc54a\ub294\ub2e4.
\nType \u2161\ub294 \uc778\uc2dd\uc790\ub9ac\uac00 \ud2b9\uc774\uc801\uc774\uace0 \uc81c\ud55c\uc790\ub9ac \ub610\ud55c \uc8fc\ubcc0\uc758 \uc5fc\uae30\ub85c \ud2b9\uc774\uc801\uc73c\ub85c \uc791\uc6a9\ud55c\ub2e4. \uc2e4\ud5d8\uc2e4\uc5d0\uc11c \uc8fc\ub85c \uc0ac\uc6a9\ud558\ub294 \uac83\uc774 \uc774 \uc720\ud615\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c 2. \uc2e4\ud5d8 \ub3c4\uad6c 3. \uc774\ub860 4. \uc2e4\ud5d8 \ubaa9\ud45c 5. \uc2e4\ud5d8 \ubc29\ubc95 6. \uacb0\uacfc 7. \uace0\ucc30 8. \ucd9c\ucc98 \ubcf8\ubb38\ub0b4\uc6a9 2. \uc2e4\ud5d8 \ub3c4\uad6c – \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301, EP tube , tube rack, \ub77c\ud14d\uc2a4 \uc7a5\uac11, microwave, UV lamp, \uc804\uae30\uc601\ub3d9\uc7a5\uce58, shaking incubator, vortex mixer, centrifuge, \uc2a4\ud2f0\ub85c\ud3fc \ubc15\uc2a4, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[3284,20276,16630,20274,20273,20275,20020,20021,8319,20277],"class_list":["post-15213","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-agarose-gel","tag-blunt-end","tag-gel-electrophoresis","tag-palindrome","tag-restriction-enzyme","tag-sticky-end","tag-20020","tag-20021","tag-8319","tag-20277"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15213","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=15213"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15213\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=15213"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=15213"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=15213"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}