{"id":15214,"date":"2025-09-04T09:01:13","date_gmt":"2025-09-04T00:01:13","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15214\/"},"modified":"2025-09-04T09:01:13","modified_gmt":"2025-09-04T00:01:13","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%981-a-%ec%9e%90%eb%a3%8c-6-genomic-dna-prep-gel-electrophoresis","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/09\/04\/15214\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(1) A \uc790\ub8cc) 6. Genomic DNA prep & Gel Electrophoresis"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c
\n2. \uc2e4\ud5d8 \ub3c4\uad6c
\n3. \uc774\ub860
\n4. \uc2e4\ud5d8 \ubaa9\ud45c
\n5. \uc2e4\ud5d8 \ubc29\ubc95
\n6. \uacb0\uacfc
\n7. \uace0\ucc30
\n8. \ucd9c\ucc98<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

2. \uc2e4\ud5d8 \ub3c4\uad6c
\n– Sample : mouse tail
\n– \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301, EP tube , tube rack, \ub77c\ud14d\uc2a4 \uc7a5\uac11, nanodrop, UV lamp, \uc804\uae30\uc601\ub3d9\uc7a5\uce58, shaking incubator, centrifuge
\n– \uc2dc\uc57d : lysis buffer(1M Tris HCl, 0.5M EDTA, 010% SDS, 5M NaCl), proteinase K, isopropanol, agarose powder, 1X TAE buffer, 70% DEPC-EtOH, DNA size marker, DW, 6X loading dye, gel electrophoresis buffer, EtBr<\/p>\n

3. \uc774\ub860
\n\u2460 Genomic DNA
\nGenomic DNA\ub294 \uae34 \uc774\uc911\uc0ac\uc2ac\ub85c\uc11c \uc138\ud3ec \ud575 \ub0b4\uc5d0 \uc874\uc7ac\ud55c\ub2e4. \uac8c\ub188 DNA \ub610\ub294 gDNA\ub294 \uc720\uae30\uccb4\uc758 \uc5fc\uc0c9\uccb4 DNA\ub85c \uc720\uc804 \ubb3c\uc9c8\uc758 \ub300\ubd80\ubd84\uc744 \ub098\ud0c0\ub0b8\ub2e4. \uc138\ud3ec\uac00 \ubcf8\ub798 \uac00\uc9c0\uace0 \uc788\ub294 DNA\ub85c, \uc678\ubd80\uc5d0\uc11c \ub4e4\uc5b4\uc628 plasmic DNA\uac19\uc740 extra genomic DNA\uac00 \uc544\ub2cc chromosome DNA\uc774\ub2e4. \uc138\ud3ec\uac00 \uc0b4\uc544\uac00\ub294\ub370 \ud544\uc694\ud55c \ubaa8\ub4e0 \uc720\uc804\uc815\ubcf4\ub4e4\uc744 \ub2f4\uace0 \uc788\ub294 DNA\uc774\ub2e4. \uc9c4\ud575\uc138\ud3ec \uc0dd\ubb3c\uc758 \uacbd\uc6b0, \ud575 \ub0b4\uc5d0 \uc874\uc7ac\ud558\ub294 chromosome DNA \ubfd0\ub9cc \uc544\ub2c8\ub77c, \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\ub098 \uc5fd\ub85d\uccb4 genomic DNA\ub97c \ubaa8\ub450 \ud3ec\ud568\ud558\uae30 \ub54c\ubb38\uc5d0 \uc9c4\ud575\uc138\ud3ec\uac00 \uac00\uc9c0\uace0 \uc788\ub294 total genomic DNA\ub97c \uc758\ubbf8\ud558\uae30\ub3c4 \ud55c\ub2e4.
\n\u2461 DNA \ucd94\ucd9c
\nGenomic DNA\uc640 plasmic DNA \ucd94\ucd9c\uc758 \uae30\ubcf8 \uc6d0\ub9ac\ub294 \uac19\uc73c\ub098, plasmid DNA\ub294 genomic DNA\ub85c\ubd80\ud130 plasmid\ub97c \ubd84\ub9ac\ud558\uae30 \uc704\ud55c \ub2e8\uacc4\ub97c \uac70\uccd0\uc57c \ud55c\ub2e4. \uc138\ud3ec\ub85c\ubd80\ud130 DNA \ucd94\ucd9c\uc740 \ud06c\uac8c \uc138\ud3ec\uc758 \uc218\ud655(cell harvest), \uc138\ud3ec\uc758 \ud30c\uad34(cell lysis), DNA \ucd94\ucd9c\uacfc \uc815\uc81c(DNA purification)\uc758 \uacfc\uc815\uc73c\ub85c \ub098\ub25c\ub2e4.
\n1) \uc138\ud3ec\uc758 \uc218\ud655 : \uc77c\ubc18\uc801\uc73c\ub85c \uc6d0\uc2ec\ubd84\ub9ac\ub97c \uc774\uc6a9\ud558\uc5ec \uc138\ud3ec\ub97c \uc218\ud655\ud55c\ub2e4.
\n2) \uc138\ud3ec\uc758 \ud30c\uad34
\n\uc138\ud3ec\ub85c\ubd80\ud130 DNA\ub97c \ubd84\ub9ac\ud558\uae30 \uc704\ud574\uc11c\ub294 \uc138\ud3ec \ub0b4 DNA\uac00 \uc138\ud3ec \ubc16\uc73c\ub85c \uc720\ucd9c\ub420 \uc218 \uc788\ub3c4\ub85d \uc138\ud3ec\ubcbd, \uc138\ud3ec\ub9c9\uc744 \ubd84\ud574\ud574\uc57c\ud55c\ub2e4. \uc774\ub294 \ubb3c\ub9ac\uc801, \ud654\ud559\uc801 \ubc29\ubc95\uc73c\ub85c \ub098\ub25c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \uc77c\uc2dc \ubc0f \uc7a5\uc18c 2. \uc2e4\ud5d8 \ub3c4\uad6c 3. \uc774\ub860 4. \uc2e4\ud5d8 \ubaa9\ud45c 5. \uc2e4\ud5d8 \ubc29\ubc95 6. \uacb0\uacfc 7. \uace0\ucc30 8. \ucd9c\ucc98 \ubcf8\ubb38\ub0b4\uc6a9 2. \uc2e4\ud5d8 \ub3c4\uad6c – Sample : mouse tail – \uc2e4\ud5d8 \uae30\uc790\uc7ac : \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab & \ub9c8\uc774\ud06c\ub85c \ud53c\ud3ab \ud301, EP tube , tube rack, \ub77c\ud14d\uc2a4 \uc7a5\uac11, nanodrop, UV lamp, \uc804\uae30\uc601\ub3d9\uc7a5\uce58, shaking incubator, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[3284,20282,20280,20279,16630,20278,20281,20020,20021,8319],"class_list":["post-15214","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-agarose-gel","tag-depc-etoh","tag-dna-denaturation","tag-dna-","tag-gel-electrophoresis","tag-genomic-dna-prep","tag-nanodrop","tag-20020","tag-20021","tag-8319"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15214","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=15214"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/15214\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=15214"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=15214"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=15214"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}