{"id":1763,"date":"2025-04-23T09:00:40","date_gmt":"2025-04-23T00:00:40","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/04\/23\/1763\/"},"modified":"2025-04-23T09:00:40","modified_gmt":"2025-04-23T00:00:40","slug":"%ec%8b%a4%ed%97%98%ec%8b%a4%ec%8a%b5%eb%b3%b4%ea%b3%a0%ec%84%9c_-agarose-gel-%ec%a0%84%ea%b8%b0%ec%98%81%eb%8f%99","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/04\/23\/1763\/","title":{"rendered":"\uc2e4\ud5d8\uc2e4\uc2b5\ubcf4\uace0\uc11c_ Agarose gel \uc804\uae30\uc601\ub3d9"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc0dd\uba85\uacf5\ud559 \uae30\ucd08\uc2e4\ud5d8I \uc2e4\ud5d8\uc2e4\uc2b5 \ubcf4\uace0\uc11c (1)
\n1) \uc2e4\ud5d8\uc8fc\uc81c \ubc0f \ubaa9\ud45c
\n2) \uc0ac\uc6a9\ud55c \uc2e4\uc2b5\uc7a5\ube44
\n3) \uc2e4\ud5d8\uc6d0\ub9ac
\n4) \uc2e4\ud5d8\uacfc\uc815<\/p>\n

2. \uc0dd\uba85\uacf5\ud559 \uae30\ucd08\uc2e4\ud5d8I \ud559\uc2b5\ud65c\ub3d9\ubcf4\uace0\uc11c (2)
\n1) \uc2e4\ud5d8\uacb0\uacfc
\n2) \uace0\ucc30
\n3) \uc88b\uc558\ub358\uc810
\n4) \uc544\uc26c\uc6e0\ub358\uc810 (\uac74\uc758\uc0ac\ud56d)<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uc2e4\ud5d8\uc8fc\uc81c \ubc0f \ubaa9\ud45c
\n\uc544\uac00\ub85c\uc2a4\ub97c \uc774\uc6a9\ud558\uc5ec \ubbf8\uc9c0\uc758 DNA\ub97c \ubd84\ub9ac\ud558\uace0 \ubd84\uc11d\ud55c\ub2e4.
\nAgarose gel \uc744 \uc9c1\uc811 \ub9cc\ub4e4 \uc218 \uc788\ub2e4. <\/p>\n

\uc0ac\uc6a9\ud55c \uc2e4\uc2b5\uc7a5\ube44
\nAgarose gel, EtBr, Agarose, UV transilluminator, \ube44\ucee4, measuring cylinder, micropipette, tip, DNA sample, Loading dye, casting tray, \uc804\uc790\ub808\uc778\uc9c0, comb, well, TAE buffer, \ud50c\ub77c\uc2a4\ud06c<\/p>\n

\uc2e4\ud5d8\uc6d0\ub9ac
\n\uc804\ub958\ub97c \ud758\ub7ec\uc8fc\uba74 \uc74c\uc804\ud558\ub97c \uac00\uc9c4 DNA\uac00 \ub450 \uc804\uadf9 \uc0ac\uc774\uc5d0\uc11c +\uadf9 \ucabd\uc73c\ub85c \uc774\ub3d9\ud558\ub294 \uc6d0\ub9ac\uc774\ub2e4. <\/p>\n

\uc2e4\ud5d8\uacfc\uc815
\n [1] Agarose gel \uc804\uae30\uc601\ub3d9
\n1. Loading dye\ub97c \ud55c tube \ub2f9 10ul \uc529 DNA sample(P, PR, S) \uc5d0 \ub123\uc5b4\uc900\ub2e4.
\n *Loading dye\ub97c \ucca8\uac00\ud558\ub294 \uc774\uc720*
\n \u2780Loading dye: \uc804\uae30\uc601\ub3d9 \uc2dc \uc0d8\ud50c\uacfc \ud568\uaed8 \uc11e\uc5b4 \ub0b4\ub9ac\ub294 \uc5fc\ub8cc
\n \u2781\uc0d8\ud50c\uc5d0 \ubc00\ub3c4\ub97c \ucd94\uac00\ud558\uc5ec DNA\uac00 buffer \uc18d\uc5d0 \ud655\uc0b0\ub418\uc9c0 \uc54a\uace0, \ubc11\uc73c\ub85c \uac00\ub77c\uc549\uc744 \uc218 \uc788 \ub3c4\ub85d \ud55c\ub2e4.
\n \u2782\uc0c9\uc0c1\uc744 \uc81c\uacf5\ud558\uc5ec DNA\uc758 \uc704\uce58\ub97c \ud55c\ub208\uc5d0 \uc54c\uc544\ubcfc \uc218 \uc788\uac8c \ud55c\ub2e4.<\/p>\n

2. \uac01\uc790 \uc870\uc6d0\ub4e4\uc740 1kb, 100bp, P, PR, S\ub97c 5ul\ub85c \uc67c\ucabd, \uc624\ub978\ucabd agarose gel\uc758 \uc0c1\ub2e8 \ud648\uc5d0 \uac01\uac01 \ub123\uc5b4\uc900\ub2e4. (\uc870\uc6d0 \ubaa8\ub450 \uc67c\ucabd, \uc624\ub978\ucabd \uac01 \ud55c \ubc88\uc529 \uc2e4\uc2dc)
\n3. \uc804\uc6d0\uc7a5\uce58\ub97c 100V\ub85c 60\ubd84 \uc124\uc815\ud574\uc900\ub2e4. (running:\uc804\uae30\uc7a5\uc744 \uac78\uc5b4\uc8fc\uace0 \ub760\uac00 \ub098\uc62c \ub54c \uae4c\uc9c0 \uae30\ub2e4\ub9bc\/ \uc804\uc555\uc774 \ub192\uc744\uc218\ub85d \uc774\ub3d9\uc774 \ube60\ub974\ub2e4.) ->DNA\uac00 \ud06c\uae30 \ubcc4\ub85c \ubd84\ub9ac\ub41c\ub2e4.
\n4. \ub178\ub780\uc0c9 line\uc774 \ub05d\ubd80\ubd84\uc5d0 \ub3c4\ub2ec\ud560 \ub54c\uae4c\uc9c0 10\ubd84 \uac04\uaca9\uc73c\ub85c \uad00\ucc30\ud55c\ub2e4.
\n5. UV light\ub97c \uc774\uc6a9\ud558\uc5ec \uc804\uae30\uc601\ub3d9 \uacb0\uacfc\ub97c \ud655\uc778\ud55c\ub2e4.<\/p>\n

[2] Agarose gel \ub9cc\ub4e4\uae30
\n1. TAE buffer\uc5d0 \ud30c\uc6b0\ub354 \ud615\ud0dc\uc758 \uc544\uac00\ub85c\uc2a4\ub97c \uc815\ud574\uc9c4 \ub18d\ub3c4\ub85c \ub123\ub294\ub2e4. (25ml buffer\ub97c 0.8% \ub18d\ub3c4\ub85c \ub9cc\ub4e4\uc5b4\uc57c\ud568 = 0.2g\uc758 \uc544\uac00\ub85c\uc2a4 \ud30c\uc6b0\ub354 \ud544\uc694)<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc0dd\uba85\uacf5\ud559 \uae30\ucd08\uc2e4\ud5d8I \uc2e4\ud5d8\uc2e4\uc2b5 \ubcf4\uace0\uc11c (1) 1) \uc2e4\ud5d8\uc8fc\uc81c \ubc0f \ubaa9\ud45c 2) \uc0ac\uc6a9\ud55c \uc2e4\uc2b5\uc7a5\ube44 3) \uc2e4\ud5d8\uc6d0\ub9ac 4) \uc2e4\ud5d8\uacfc\uc815 2. \uc0dd\uba85\uacf5\ud559 \uae30\ucd08\uc2e4\ud5d8I \ud559\uc2b5\ud65c\ub3d9\ubcf4\uace0\uc11c (2) 1) \uc2e4\ud5d8\uacb0\uacfc 2) \uace0\ucc30 3) \uc88b\uc558\ub358\uc810 4) \uc544\uc26c\uc6e0\ub358\uc810 (\uac74\uc758\uc0ac\ud56d) \ubcf8\ubb38\ub0b4\uc6a9 \uc2e4\ud5d8\uc8fc\uc81c \ubc0f \ubaa9\ud45c \uc544\uac00\ub85c\uc2a4\ub97c \uc774\uc6a9\ud558\uc5ec \ubbf8\uc9c0\uc758 DNA\ub97c \ubd84\ub9ac\ud558\uace0 \ubd84\uc11d\ud55c\ub2e4. Agarose gel \uc744 \uc9c1\uc811 \ub9cc\ub4e4 \uc218 \uc788\ub2e4. \uc0ac\uc6a9\ud55c \uc2e4\uc2b5\uc7a5\ube44 Agarose gel, EtBr, Agarose, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[425,3284,3129,315,2534,3217,3283,2632],"class_list":["post-1763","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-12-","tag-agarose-gel","tag-kata","tag-315","tag-2534","tag-3217","tag-3283","tag-2632"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/1763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=1763"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/1763\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=1763"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=1763"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=1763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}