{"id":18354,"date":"2025-10-06T09:00:31","date_gmt":"2025-10-06T00:00:31","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/10\/06\/18354\/"},"modified":"2025-10-06T09:00:31","modified_gmt":"2025-10-06T00:00:31","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%982-a-%ec%9e%90%eb%a3%8c-8-polyethylenimine-pei%ec%9d%84-%ec%9d%b4%ec%9a%a9%ed%95%9c-s","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/10\/06\/18354\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(2) A+ \uc790\ub8cc) 8. Polyethylenimine (PEI)\uc744 \uc774\uc6a9\ud55c siRNA \uc751\ucd95\uacfc \ub098\ub178 \uc785\uc790 \ud615\uc131"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Abstract<\/p>\n

2. Introduction<\/p>\n

3. Materials & Methods<\/p>\n

4. Results
\n 1) \uac01 N\/P ratio\uc5d0 \ub530\ub978 \ud06c\uae30 \ubc0f zeta-potential \uac12\uc744 \ube44\uad50\ud558\uace0 \uc5b4\ub5a4 \uacbd\ud5a5\uc131\uc774 \uc788\ub294\uc9c0 \ucc3e\uc544\ubcf4\uc2dc\uc624.
\n 2) \uc800\ubd84\uc790\ub7c9 PEI\uc640 \uace0\ubd84\uc790\ub7c9 PEI\ub97c \uc0ac\uc6a9\ud558\uc600\uc744 \ub54c \ub098\ub178\uc785\uc790\uc758 \ud06c\uae30\ub97c \ube44\uad50\ud558\uc2dc\uc624.<\/p>\n

5. Discussions
\n 1) siRNA\uc758 \uc791\uc6a9\uc6d0\ub9ac\uc5d0 \ub300\ud574 \uc870\uc0ac\ud558\uc5ec introduction\uc5d0 \uc4f0\uc2dc\uc624.
\n 2) N\/P ratio, PEI \ubd84\uc790\ub7c9\uc774 siRNA\/PEI \ub098\ub178\ubcf5\ud569\uccb4\uc5d0 \uc5b4\ub5a4 \uc601\ud5a5\uc744 \uc8fc\ub294\uc9c0 \ucc3e\uc544\ubcf4\uc2dc\uc624. (\ud06c\uae30 \ubc0f \ud45c\uba74 \uc804\ud558 \uc678\uc5d0\ub3c4 \uc601\ud5a5\uc744 \ubc1b\ub294 \ub2e4\ub978 \uac83\uc774 \uc788\uc73c\uba74 \ucc3e\uc544\ubcf4\uc544\ub77c.)
\n 3) \uc0c1\uc6a9\ud654\ub41c \uc591\uc774\uc628\uc131 \uc720\uc804\uc790 \uc804\ub2ec\uc6a9 \uace0\ubd84\uc790\uc5d0 \ub300\ud574 \uc880 \ub354 \uc870\uc0ac\ud558\ub77c.<\/p>\n

6. References<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uc774\ubc88 \uc2e4\ud5d8\uc5d0\uc11c\ub294 \ub300\ud45c\uc801\uc778 \uc720\uc804\uc790 \uc804\ub2ec\uc6a9 \uc591\uc774\uc628\uc131 \uace0\ubd84\uc790\uc778 polyethylenimine (PEI)\uc744 \uc774\uc6a9\ud574 siRNA\/PEI \ub098\ub178\uc785\uc790\ub97c \ub9cc\ub4e4\uace0, DLS\uc640 zeta potential \uce21\uc815\uc744 \ud1b5\ud574 \uacb0\uacfc\ub97c \ud655\uc778\ud574\ubcf4\uc558\ub2e4. \ub610\ud55c \uc800\ubd84\uc790\ub7c9 (low molecular weight 0.8 kDa) PEI\uc640 \uace0\ubd84\uc790\ub7c9 (high molecular weight 25 kDa) PEI\ub97c \uc0ac\uc6a9\ud558\uc600\uc744 \ub54c\uc758 \ub098\ub178\uc785\uc790 \ud06c\uae30\uc758 \ucc28\uc774\ub97c \ube44\uad50\ud574\ubcf4\uc558\ub2e4. \uc2e4\ud5d8 \uacb0\uacfc N\/P ratio\uac00 5, 10, 20\uc73c\ub85c \uc99d\uac00\ud568\uc5d0 \ub530\ub77c, 0.8 kDa\uc758 siRNA\/PEI \ub098\ub178 \ubcf5\ud569\uccb4\uc758 \ud06c\uae30\ub294 \uac01\uac01 303.8 d.nm, 677.4 d.nm, 452.8 d.nm\uc600\ub2e4. 25 kDa\uc758 \uacbd\uc6b0, siRNA\/PEI \ub098\ub178 \ubcf5\ud569\uccb4\uc758 \ud06c\uae30\ub294 \uac01\uac01 383.4 d.nm, 677.4 d.nm, 452.8 d.nm\uc600\ub2e4. \ub450 \uacb0\uacfc \ubaa8\ub450 \ud2b9\uc815\ud55c \uacbd\ud5a5\uc131\uc744 \ucc3e\uc744 \uc218 \uc5c6\uc5c8\ub2e4. \ud558\uc9c0\ub9cc \uc774\ub860\uc801\uc778 \uacb0\uacfc\ub294 N\/P ratio\uac00 \uc99d\uac00\ud568\uc5d0 \ub530\ub77c \ub098\ub178\uc785\uc790\uc758 \ud06c\uae30\ub294 \uac10\uc18c\ud574\uc57c \ud55c\ub2e4. \ub2e4\uc74c\uc73c\ub85c N\/P ratio\uac00 5, 10, 20\uc73c\ub85c \uc99d\uac00\ud568\uc5d0 \ub530\ub77c, 0.8 kDa\uc758 siRNA\/PEI \ub098\ub178 \ubcf5\ud569\uccb4\uc758 zeta-potential\uc740 \uac01\uac01 -0.676 mV, 0.079 mV, 6.001 mV\uc600\ub2e4. 25 kDa\uc758 \uacbd\uc6b0, siRNA\/PEI \ub098\ub178 \ubcf5\ud569\uccb4\uc758 zeta-potential\uc740 \uac01\uac01 9.33 mV, 11.8 mV, 4.74 mV\uc600\ub2e4. \ub450 \uacb0\uacfc \ubaa8\ub450 Free-siRNA\uc758 zeta-potential\uc774 -8.03 mV\uc778 \uac83\uc5d0 \ube44\ud574 \ub300\uccb4\uc801\uc73c\ub85c \uc591\uc804\ud558\uac00 \uc99d\uac00\ud558\ub294 \uacbd\ud5a5\uc131\uc744 \ubcf4\uc600\ub2e4. \ub9c8\uc9c0\ub9c9\uc73c\ub85c \ubaa8\ub4e0 N\/P ratio\uc5d0\uc11c \uc800\ubd84\uc790\ub7c9\uacfc \uace0\ubd84\uc790\ub7c9 PEI\ub97c \uc0ac\uc6a9\ud55c \ub098\ub178 \ubcf5\ud569\uccb4\uc758 \ud06c\uae30\ub97c \ube44\uad50\ud558\uc600\uc744 \ub54c, \ub300\uccb4\uc801\uc73c\ub85c \uc800\ubd84\uc790\ub7c9 PEI\ub97c \uc0ac\uc6a9\ud588\uc744 \ub54c\uac00 \uace0\ubd84\uc790\ub7c9 PEI\ub97c \uc0ac\uc6a9\ud588\uc744 \ub54c\ubcf4\ub2e4 \ub098\ub178 \uc785\uc790\uc758 \ud06c\uae30\uac00 \ub354 \ucef8\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Abstract 2. Introduction 3. Materials & Methods 4. Results 1) \uac01 N\/P ratio\uc5d0 \ub530\ub978 \ud06c\uae30 \ubc0f zeta-potential \uac12\uc744 \ube44\uad50\ud558\uace0 \uc5b4\ub5a4 \uacbd\ud5a5\uc131\uc774 \uc788\ub294\uc9c0 \ucc3e\uc544\ubcf4\uc2dc\uc624. 2) \uc800\ubd84\uc790\ub7c9 PEI\uc640 \uace0\ubd84\uc790\ub7c9 PEI\ub97c \uc0ac\uc6a9\ud558\uc600\uc744 \ub54c \ub098\ub178\uc785\uc790\uc758 \ud06c\uae30\ub97c \ube44\uad50\ud558\uc2dc\uc624. 5. Discussions 1) siRNA\uc758 \uc791\uc6a9\uc6d0\ub9ac\uc5d0 \ub300\ud574 \uc870\uc0ac\ud558\uc5ec introduction\uc5d0 \uc4f0\uc2dc\uc624. 2) N\/P ratio, PEI \ubd84\uc790\ub7c9\uc774 siRNA\/PEI \ub098\ub178\ubcf5\ud569\uccb4\uc5d0 \uc5b4\ub5a4 \uc601\ud5a5\uc744 \uc8fc\ub294\uc9c0 \ucc3e\uc544\ubcf4\uc2dc\uc624. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[13050,23376,23375,23374,23373,16034,22608,20020,20021,8319],"class_list":["post-18354","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-dls","tag-n-p-ratio","tag-pei","tag-polyethylenimine","tag-sirna","tag-yellow-journailsm","tag-zeta-potential","tag-20020","tag-20021","tag-8319"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/18354","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=18354"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/18354\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=18354"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=18354"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=18354"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}