{"id":18355,"date":"2025-10-06T09:00:33","date_gmt":"2025-10-06T00:00:33","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/10\/06\/18355\/"},"modified":"2025-10-06T09:00:33","modified_gmt":"2025-10-06T00:00:33","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%982-a-%ec%9e%90%eb%a3%8c-9-polyethylenimine-pei-%eb%82%98%eb%85%b8-%eb%b3%b5%ed%95%a9","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/10\/06\/18355\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(2) A+ \uc790\ub8cc) 9. Polyethylenimine (PEI) \ub098\ub178 \ubcf5\ud569\uccb4\ub97c \uc774\uc6a9\ud55c \uc138\ud3ec \ub0b4 siRNA \uc804\ub2ec \ubc0f RNA \uac04\uc12d \ud3c9\uac00"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/26\/data29494025-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/02\/26\/data29494025-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>1. Abstract<\/p>\n<p>2. Introduction<\/p>\n<p>3. Materials &amp; Methods<\/p>\n<p>4. Results<\/p>\n<p>5. Discussions<\/p>\n<p>6. References<\/p>\n<p>+\uc5d1\uc140 \uacc4\uc0b0 \uacfc\uc815<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p>\u2160. Abstract<br \/>\n\uc774\ubc88 \uc2e4\ud5d8\uc5d0\uc11c\ub294 \uc591\uc774\uc628\uc131 branched polyethylenimine (B-PEI)\ub97c \uc774\uc6a9\ud574 siRNA\/PEI \ub098\ub178\uc785\uc790\ub97c \ub9cc\ub4e4\uace0, GFP-silencing siRNA\ub97c \uc554\uc138\ud3ec \ub0b4\ub85c \uc804\ub2ec\ud55c\ub2e4. \uc774\ub97c \ud1b5\ud574 RNA interference (RNAi) \uac04\uc12d \ud6a8\uc728\uc744 \uc138\ud3ec \ub0b4 GFP \ubc1c\ud604\ub7c9\uc758 \uac10\uc18c\ub85c \ud655\uc778\ud55c\ub2e4. \uc774 \ub54c \ub098\ub178\ubcf5\ud569\uccb4\uc758 N\/P ratio\uc5d0 \ub530\ub978 RNA \uac04\uc12d \ud6a8\uc728\uc5d0 \ub300\ud574\uc11c\ub3c4 \uc0b4\ud3b4\ubcf8\ub2e4. \uc2e4\ud5d8 \uacb0\uacfc\ub294 \ub450 \uac00\uc9c0 \uce21\uc815\uc744 \uc774\uc6a9\ud558\uc5ec \ubd84\uc11d\ud558\uc600\ub2e4. \uba3c\uc800 \ud615\uad11\ud604\ubbf8\uacbd\uc73c\ub85c \uc5bb\uc740 \ud615\uad11\uc774\ubbf8\uc9c0\ub97c \ubd84\uc11d\ud588\uc744 \ub54c, Control\uacfc Free siRNA sample\uc5d0\uc11c\ub294 \ud615\uad11\uc774 \ube44\uc2b7\ud55c \uc815\ub3c4\ub85c \uad00\ucc30\ub418\uc5c8\uace0 N\/P ratio=5\uc5d0 \ube44\ud574 N\/P ratio=20\uc758 \ud615\uad11\uc774 \uc801\uac8c \ubcf4\uc600\ub2e4. \uc774\ub97c \ud1b5\ud574 RNA \uac04\uc12d \ud6a8\uc728\uc740 N\/P ratio\uac00 \ub192\uc544\uc9d0\uc5d0 \ub530\ub77c \uc99d\uac00\ud588\uc73c\uba70, GFP \ubc1c\ud604\uc774 \uac10\uc18c\ud588\ub2e4\ub294 \uac83 \uc54c \uc218 \uc788\uc5c8\ub2e4. \ub2e4\uc74c\uc73c\ub85c flow cytometer\ub85c \uce21\uc815\ud558\uc5ec \ub3c4\ucd9c\ud55c N\/P ratio\uc5d0 \ub530\ub978 GFP \ubc1c\ud604\ub7c9(%)\uc740 control, free siRNA, N\/P=5, N\/P=20\uc5d0\uc11c \uac01\uac01 100%, 102.5%, 114.3%, 57.8%\uc600\ub2e4. GFP \ubc1c\ud604\ub7c9\uc740 N\/P=5, free siRNA, control, N\/P=20 \uc21c\uc73c\ub85c \uac10\uc18c\ud588\ub2e4. \uc774\ub97c \ud1b5\ud574, GFP \ubc1c\ud604\ub7c9\uc740 N\/P ratio=5\uc77c \ub54c\ubcf4\ub2e4 N\/P ratio=20\uc77c \ub54c \ud655\uc5f0\ud558\uac8c \uac10\uc18c\ud558\ub294 \uac83\uc744 \uc54c \uc218 \uc788\uc5c8\ub2e4.<\/p>\n<p>\u2161. Introduction<br \/>\n&#8211; \uc2e4\ud5d8 \ubaa9\ud45c : \uc591\uc774\uc628\uc131 branched polyethylenimine (B-PEI)\ub97c \uc774\uc6a9\ud574 GFP-silencing siRNA\ub97c \uc554\uc138\ud3ec \ub0b4\ub85c \uc804\ub2ec\ud558\uace0 RNA interference (RNAi) \uac04\uc12d \ud6a8\uc728\uc744 \uc138\ud3ec \ub0b4 GFP \ubc1c\ud604\ub7c9\uc758 \uac10\uc18c\ub97c \ud1b5\ud574 \ud655\uc778\ud55c\ub2e4. \uc774 \ub54c \ub098\ub178\ubcf5\ud569\uccb4\uc758 N\/P ratio\uc5d0 \ub530\ub978 RNA \uac04\uc12d \ud6a8\uc728\uc5d0 \ub300\ud574\uc11c\ub3c4 \uc0b4\ud3b4\ubcf8\ub2e4.<br \/>\n1. N\/P ratio\uc5d0 \ub530\ub978 Branched PEI\uc758 RNA \uac04\uc12d \ud6a8\uc728 \ubcc0\ud654<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/29494025\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 1. Abstract 2. Introduction 3. Materials &amp; Methods 4. Results 5. Discussions 6. References +\uc5d1\uc140 \uacc4\uc0b0 \uacfc\uc815 \ubcf8\ubb38\ub0b4\uc6a9 \u2160. Abstract \uc774\ubc88 \uc2e4\ud5d8\uc5d0\uc11c\ub294 \uc591\uc774\uc628\uc131 branched polyethylenimine (B-PEI)\ub97c \uc774\uc6a9\ud574 siRNA\/PEI \ub098\ub178\uc785\uc790\ub97c \ub9cc\ub4e4\uace0, GFP-silencing siRNA\ub97c \uc554\uc138\ud3ec \ub0b4\ub85c \uc804\ub2ec\ud55c\ub2e4. \uc774\ub97c \ud1b5\ud574 RNA interference (RNAi) \uac04\uc12d \ud6a8\uc728\uc744 \uc138\ud3ec \ub0b4 GFP \ubc1c\ud604\ub7c9\uc758 \uac10\uc18c\ub85c \ud655\uc778\ud55c\ub2e4. \uc774 \ub54c \ub098\ub178\ubcf5\ud569\uccb4\uc758 N\/P ratio\uc5d0 \ub530\ub978 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[23378,23381,23377,23379,23376,23380,20020,20021,8319],"class_list":["post-18355","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-b-pei","tag-flow-cytometer","tag-gfp","tag-gfp-silencing-sirna","tag-n-p-ratio","tag-rna-","tag-20020","tag-20021","tag-8319"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/18355","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=18355"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/18355\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=18355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=18355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=18355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}