{"id":23122,"date":"2025-11-22T09:01:52","date_gmt":"2025-11-22T00:01:52","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/11\/22\/23122\/"},"modified":"2025-11-22T09:01:52","modified_gmt":"2025-11-22T00:01:52","slug":"%ea%b8%b0%ea%b8%b0%eb%b6%84%ec%84%9d%ec%8b%a4%ed%97%98-hplc","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/11\/22\/23122\/","title":{"rendered":"\uae30\uae30\ubd84\uc11d\uc2e4\ud5d8 HPLC"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\ubaa9\uc801
\n2. \uc2e4\ud5d8\uc7ac\ub8cc \ubc0f \uc2e4\ud5d8\ubc29\ubc95
\n3. \uc774\ub860
\n4. \uacb0\uacfc \ubc0f \uace0\ucc30<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc2e4\ud5d8\ubaa9\uc801 :
\nHPLC\ub97c \uc774\uc6a9\ud590\uc5ec \uc57d\ubb3c\uc744 \uc815\ub7c9\ud560 \uc218 \uc788\ub2e4.
\n HPLC \uc0ac\uc6a9\ubc95\uc744 \uc775\ud790 \uc218 \uc788\ub2e4.<\/p>\n

2.\uc2e4\ud5d8\uc7ac\ub8cc \ubc0f \uc2e4\ud5d8\ubc29\ubc95
\n \uc2e4\ud5d8\uc7ac\ub8cc : HPLC, metformin \uc2a4\ud0e0\ub2e4\ub4dc \uc6a9\uc561, \uc774\ub3d9\uc0c1(D.W : MeOH = 70 : 30), C18(\uace0\uc815\uc0c1)
\n \uc2e4\ud5d8\ubc29\ubc95
\n 1) \uc774\ub3d9\uc0c1 D.W\uc640 MeOH\ub97c 70 : 30\uc73c\ub85c \uc11e\uc5b4 \uc81c\uc870\ud55c\ub2e4.
\n 2) \uc774\ub3d9\uc0c1\uacfc \uace0\uc815\uc0c1\uc744 \ud655\uc778\ud55c\ub2e4.
\n 3) HPLC \uc804\uc6d0\uc744 \ucf20\ub2e4.
\n [Mobile phase] D.W. : MeOH= 70 : 30
\n [Column] C18 Column (5um, 4.6 mm * 150 mm)
\n [Stop time] 6 min
\n [Flow rate] 1mL\/min
\n [Injection volume] 20uL
\n [Wavelength] 232 nm \ub85c \ud504\ub85c\uadf8\ub7a8\uc73c\ub85c \uc124\uc815\ud574 \uc900\ub2e4.
\n 4) \uc774\ub3d9\uc0c1\uc758 \uae30\ud3ec\ub97c \uc81c\uac70\ud574 \uc900\ub2e4.
\n 5) \uc2dc\ub8cc\ub97c \uc8fc\uc785\ud55c\ub2e4.
\n 6) \uae30\uae30 \uc548\uc815\ud654 \uc2dc\ud0a8\ub2e4.
\n 7) Blank \uce21\uc815\ud55c \ub4a4 standard\uc640 Sample\uc744 \ub123\uc5b4\uc11c \uce21\uc815\ud55c \ub4a4 \ubd84\uc11d\ud55c\ub2e4.
\n 8) \uc131\ubd84 \ubd84\uc11d\uacfc, \uc591\uc744 \ubd84\uc11d\ud55c\ub2e4.<\/p>\n

3.\uc774\ub860
\n 1) HPLC \uad6c\uc131
\n (1) \ud0c8\uae30\uc7a5\uce58 : \uc720\ub7c9\uacfc \uc555\ub825\uc5d0 \uc601\ud5a5\uc744 \uc904 \uc218 \uc788\ub294 \uc774\ub3d9\uc0c1 \uc911\uc758, \uc6a9\uc874\uc0b0\uc18c, \uc9c8\uc18c, \uae30\ud3ec
\n \ub4f1\uc744 \uc5c6\uc560\uae30 \uc704\ud574 \uc0ac\uc6a9\ud558\ub294 \uc7a5\uce58
\n > \uc7a5\ube44\uc5d0 \uc5f0\uacb0\ub41c \ud29c\ube59\uc774 \uc587\uae30 \ub54c\ubb38\uc5d0 \uc815\ud655\ud55c \uacb0\uacfc \uac12\uc744 \uc5bb\ub294\ub370 \uc601\ud5a5\uc744 \ub07c\uccd0 \uae30\ud3ec\ub97c
\n \uc5c6\uc560\ub294 \uac83\uc774 \ub9e4\uc6b0 \uc911\uc694\ud568.
\n (2) \ud38c\ud504 : \uc774\ub3d9\uc0c1\uc744 \uc8fc\uc785\ubd80, \uceec\ub7fc, \uac80\ucd9c\uae30\uae4c\uc9c0 \uc6b4\uc1a1\ud558\uae30 \uc704\ud55c \uace0\uc555 \ud38c\ud504
\n > \uc77c\uc815\ud55c \uc720\uc18d\uc774\ub098 \uc555\ub825\uc744 \uc720\uc9c0\ud558\ub294 \uc694\uc18c\uc774\uba70 \uc815\ud655\ud55c \uacb0\uacfc\uac12, \ub192\uc740 \uc7ac\ud604\uc131 \ub3c4\ucd9c\uc5d0
\n \uac00\uc7a5 \ud544\uc694\ud55c \ubd80\ubd84\uc774\ub2e4.
\n (3) \uc2dc\ub8cc\uc8fc\uc785\uae30 : \uc804\uac1c\uc7ac(\uc774\ub3d9\uc0c1)\uc5d0 \uc2dc\ub8cc\ub97c \ub354\ud574 \uceec\ub7fc\uc73c\ub85c \ubcf4\ub0b4\uc8fc\ub294 \uc5ed\ud560\uc744 \ud55c\ub2e4.
\n (4) \uce7c\ub7fc : \uc2dc\ub8cc\uc758 \ubd84\ub9ac\uac00 \uc77c\uc5b4\ub098\ub294 \ubd80\ubd84\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc2e4\ud5d8\uc7ac\ub8cc \ubc0f \uc2e4\ud5d8\ubc29\ubc95 3. \uc774\ub860 4. \uacb0\uacfc \ubc0f \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 1. \uc2e4\ud5d8\ubaa9\uc801 : HPLC\ub97c \uc774\uc6a9\ud590\uc5ec \uc57d\ubb3c\uc744 \uc815\ub7c9\ud560 \uc218 \uc788\ub2e4. HPLC \uc0ac\uc6a9\ubc95\uc744 \uc775\ud790 \uc218 \uc788\ub2e4. 2.\uc2e4\ud5d8\uc7ac\ub8cc \ubc0f \uc2e4\ud5d8\ubc29\ubc95 \uc2e4\ud5d8\uc7ac\ub8cc : HPLC, metformin \uc2a4\ud0e0\ub2e4\ub4dc \uc6a9\uc561, \uc774\ub3d9\uc0c1(D.W : MeOH = 70 : 30), C18(\uace0\uc815\uc0c1) \uc2e4\ud5d8\ubc29\ubc95 1) \uc774\ub3d9\uc0c1 D.W\uc640 MeOH\ub97c 70 : 30\uc73c\ub85c \uc11e\uc5b4 \uc81c\uc870\ud55c\ub2e4. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[4587,12179,28597],"class_list":["post-23122","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-hplc","tag-12179","tag--hplc"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/23122","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=23122"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/23122\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=23122"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=23122"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=23122"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}