{"id":26512,"date":"2025-12-26T09:01:48","date_gmt":"2025-12-26T00:01:48","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/26\/26512\/"},"modified":"2025-12-26T09:01:48","modified_gmt":"2025-12-26T00:01:48","slug":"%ec%9c%a0%ec%a0%84-%eb%b0%9c%ec%83%9d-%ec%83%9d%eb%ac%bc%ed%95%99-%ec%8b%a4%ed%97%98-%eb%a0%88%ed%8f%ac%ed%8a%b8-cellular-senescence-immunofluorescence","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/26\/26512\/","title":{"rendered":"\uc720\uc804 \ubc1c\uc0dd \uc0dd\ubb3c\ud559 \uc2e4\ud5d8 \ub808\ud3ec\ud2b8 Cellular Senescence Immunofluorescence"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\u2160. Introduction
\n\u2161. Materials and Methods
\n\u2162. Results
\n\u2163. Discussion
\n\u2164. References<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. DPP4 protein<\/p>\n

DPP4(dipeptidul peptidase 4)\ub294 \ub2e4\uc591\ud55c \uc7a5\uae30\uc640 \uc138\ud3ec\uc5d0\uc11c \ub110\ub9ac \ubc1c\ud604\ub418\ub294 \ub9c9 \uad00\ud1b5\ud615 \ub2f9\ub2e8\ubc31\uc9c8\uc774\ub2e4. DPP4\ub294 \ud608\uc7a5\uacfc \uccb4\uc561\uc5d0\uc11c\ub3c4 \uc6a9\ud574\uc131 \ud615\ud0dc\ub85c \uc874\uc7ac\ud560 \uc218 \uc788\ub2e4[1].\ub610\ud55c DPP4\ub294 \ub178\ud654 \uc138\ud3ec\uc758 \ubc14\uc774\uc624 \ub9c8\ucee4\ub85c\uc11c \uc791\uc6a9\ud560 \uc218 \uc788\ub2e4. DPP4\ub294 \ub178\ud654\ub41c \uc778\uac04 \uc774\ubc30\uccb4 \uc12c\uc720\uc544\uc138\ud3ec(human diploid fibroblasts)\uc778 WI-38 HDF \uc758 \ud45c\uba74\uc5d0\uc11c \uc120\ud0dd\uc801\uc73c\ub85c \ubc1c\ud604\ub418\ub294 \ub2e8\ubc31\uc9c8\uc774\ub2e4[2].<\/p>\n

<\uadf8\ub9bc 1> \uc99d\uc2dd \ubc0f \ub178\ud654 WI-38 HDF \uc138\ud3ec \ud45c\uba74\uc5d0\uc11c\uc758 DPP4 \ubc1c\ud604[2]<\/p>\n

<\uadf8\ub9bc1>\uc5d0\uc11c \ubcf4\ub294\uac83\uacfc \uac19\uc774 \uc80a\uc740 \uc138\ud3ec(P)\uc5d0\uc11c\ub294 DPP4\uac00 \uc5fc\uc0c9\ub418\uc9c0 \uc54a\uc740 \uac83\uc73c\ub85c \ubcf4\uc544 \uac70\uc758 \ubc1c\ud604\ub418\uc9c0 \uc54a\uc558\uace0, \ub178\ud654 \uc138\ud3ec(S)\uc5d0\uc120 DPP4\uac00 \ube68\uac1b\uac8c \uc5fc\uc0c9\ub418\uc5b4 \ubc1c\ud604\ud568\uc744 \ud655\uc778\ud560 \uc218 \uc788\ub2e4.
\n\ub610\ud55c DPP4\ub294 \ub178\ud654 \uc138\ud3ec\ub294 \ud56d\uccb4 \uc758\uc874\uc131 \uc138\ud3ec \ub9e4\uac8c \uc138\ud3ec\ub3c5\uc131(antibody-dependent cell-mediated cytotoxicity, ADCC)\uc5d0 \uc758\ud574 \uc6b0\uc120\uc801\uc73c\ub85c \uc81c\uac70\ub418\ub294\ub370, \uc774\ub294 <\uadf8\ub9bc 3>\uc5d0 \ub098\ud0c0\ub0b4\ub294 \uac83\ucc98\ub7fc \uc138\ud3ec \ud45c\uba74\uc5d0 DPP4\uac00 \uc874\uc7ac\ud558\uae30 \ub54c\ubb38\uc5d0 \ud56d-DPP4 \ud56d\uccb4\ub97c \uc778\uc2dd\ud558\ub294 NK cell\uc5d0 \uc758\ud55c \ud30c\uad34\uc758 \ud45c\uc801\uc774 \ub418\uc5c8\uae30 \ub54c\ubb38\uc774\ub2e4[2]. <\/p>\n

<\uadf8\ub9bc 2> ADCC \uc791\uc6a9 \uae30\uc791[3]<\/p>\n

<\uadf8\ub9bc 3> \ub178\ud654 \uc138\ud3ec\uc758 \uc120\ud0dd\uc801 \uc81c\uac70[2]
\n\uc989 \uc138\ud3ec\uac00 \ub178\ud654\ud560\uc218\ub85d \uc138\ud3ec \ud45c\uba74\uc758 DPP4\uac00 \uc99d\uac00\ud558\uac8c \ub418\ub294\ub370 \uc774\ub294 ADCC\uc5d0 \uc758\ud574 \ub178\ud654 \uc138\ud3ec\ub97c \uc120\ud0dd\uc801\uc73c\ub85c \uc81c\uac70\ud558\uac8c \ub41c\ub2e4.
\nADCC\ub294 \uc554\uc774\ub098 \ubc14\uc774\ub7ec\uc2a4\uac00 \uac10\uc5fc\ub41c \ud0c0\uac9f \uc138\ud3ec\ub97c \uc6a9\ud574\ud558\uae30 \uc704\ud55c \uba74\uc5ed\uc138\ud3ec\uc758 \ud65c\uc131\ud654\ub97c \uc720\ub3c4\ud558\ub294 \uc138\ud3ec \ub9e4\uac8c \uba74\uc5ed \ubc29\uc5b4 \ub9e4\ucee4\ub2c8\uc998\uc774\ub2e4[3]. \uc774 \uacbd\uc6b0\uc5d0\uc11c\ub294 \ud0c0\uac9f\uc138\ud3ec\uac00 \ub178\ud654 \uc138\ud3ec\ub85c, NK cell\uc774 \uc138\ud3ec \ud45c\uba74\uc758 \ud56d-DPP4 \ud56d\uccb4\ub97c \uc778\uc2dd\ud558\uc5ec \uc138\ud3ec\ub97c \uc81c\uac70\ud558\ub294 \uac83\uc774\ub2e4. \uc774\ub7ec\ud55c \uba54\ucee4\ub2c8\uc998\uc744 \uc774\uc6a9\ud574 \ub178\ud654 \uc138\ud3ec\ub97c \uc81c\uac70\ud558\uac8c \ub41c\ub2e4.<\/p>\n

\ubcf8 \uc2e4\ud5d8\uc5d0\uc11c\ub294 \uba74\uc5ed \ud615\uad11 \uc5fc\uc0c9(Immunofluorescence) \ubc29\ubc95\uc744 \uc774\uc6a9\ud558\uc5ec \ub178\ud654 \uc138\ud3ec\uc640 \uc99d\uc2dd \uc138\ud3ec\uc5d0\uc11c\uc758 DPP4 \ubc1c\ud604\uc758 \ucc28\uc774\ub97c \ub208\uc73c\ub85c \uc9c1\uc811 \ud655\uc778\ud560 \uac83\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \u2160. Introduction \u2161. Materials and Methods \u2162. Results \u2163. Discussion \u2164. References \ubcf8\ubb38\ub0b4\uc6a9 1. DPP4 protein DPP4(dipeptidul peptidase 4)\ub294 \ub2e4\uc591\ud55c \uc7a5\uae30\uc640 \uc138\ud3ec\uc5d0\uc11c \ub110\ub9ac \ubc1c\ud604\ub418\ub294 \ub9c9 \uad00\ud1b5\ud615 \ub2f9\ub2e8\ubc31\uc9c8\uc774\ub2e4. DPP4\ub294 \ud608\uc7a5\uacfc \uccb4\uc561\uc5d0\uc11c\ub3c4 \uc6a9\ud574\uc131 \ud615\ud0dc\ub85c \uc874\uc7ac\ud560 \uc218 \uc788\ub2e4[1].\ub610\ud55c DPP4\ub294 \ub178\ud654 \uc138\ud3ec\uc758 \ubc14\uc774\uc624 \ub9c8\ucee4\ub85c\uc11c \uc791\uc6a9\ud560 \uc218 \uc788\ub2e4. DPP4\ub294 \ub178\ud654\ub41c \uc778\uac04 \uc774\ubc30\uccb4 \uc12c\uc720\uc544\uc138\ud3ec(human diploid fibroblasts)\uc778 WI-38 HDF \uc758 \ud45c\uba74\uc5d0\uc11c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[33153,2619,33152,33154],"class_list":["post-26512","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-immunofluorescence","tag-2619","tag-33152","tag-33154"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26512","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=26512"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26512\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=26512"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=26512"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=26512"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}