{"id":26638,"date":"2025-12-28T09:00:19","date_gmt":"2025-12-28T00:00:19","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/28\/26638\/"},"modified":"2025-12-28T09:00:19","modified_gmt":"2025-12-28T00:00:19","slug":"%eb%b6%84%ec%9e%90%ec%83%9d%eb%ac%bc%ec%83%9d%ed%99%94%ed%95%99%ec%8b%a4%ed%97%98_iptg-%eb%8c%80%ec%9e%a5%ea%b7%a0%ec%97%90%ec%84%9c-%ec%9e%ac%ec%a1%b0%ed%95%a9-%eb%8b%a8%eb%b0%b1%ec%a7%88%ec%9d%98","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/28\/26638\/","title":{"rendered":"\ubd84\uc790\uc0dd\ubb3c\uc0dd\ud654\ud559\uc2e4\ud5d8_IPTG, \ub300\uc7a5\uade0\uc5d0\uc11c \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8\uc758 \uc0dd\uc0b0\uacfc Western blot\uc5d0 \uad00\ud55c \uc2e4\ud5d8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\u2160. Introduction
\n\u2161. Materials & Methods
\n\u2162. Result
\n\u2163. Conclusion(Discussion)
\n\u2164. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n


\n IPTG\ub780, Isopropyl-\u03b2-D-1-thiogalactopyranoside\uc758 \uc57d\uae30\ub85c \ub300\uc7a5\uade0 lactose operon\uc758 \ud6a8\uc18c\ud569\uc131\uc744 \uc720\ub3c4\ud558\ub294 \uac15\ub825\ud55c \uc720\ub3c4\ubb3c\uc9c8\uc774\ub2e4. \ubcf4\ud1b5 \uc720\ub3c4\ubb3c\uc9c8\uc740 operon\uc758 \ud65c\ub3d9\uc5d0 \uc758\ud574 \ub9cc\ub4e4\uc5b4\uc9c0\ub294 \u03b2-D-galactosidase\uc5d0 \uc758\ud574 \ub300\uc0ac \uc774\uc6a9\ud558\ub294 \uac83\uc774 \ub9ce\uc9c0\ub9cc, IPTG\ub294 \ubd84\ud574\ub418\uc9c0 \uc54a\uc544 \ube44\ub300\uc0ac\uc131 \uc720\ub3c4\ubb3c\uc9c8\uc774\ub77c\uace0\ub3c4 \ubd88\ub9b0\ub2e4. \ub610\ud55c, \uace0\ub18d\ub3c4\ub85c \uc0ac\uc6a9\uc2dc galactoside permease\uac00 \uc5c6\ub294 \uc138\ud3ec\uc5d0\ub3c4 \ud22c\uacfc\ud560 \uc218 \uc788\uae30 \ub54c\ubb38\uc5d0 permease\uac00 \uc5c6\ub294 \uade0\uc8fc\uc5d0\uc11c\uc758 \uc720\ub3c4\uc2e4\ud5d8\uc5d0\ub3c4 \uc0ac\uc6a9\ub41c\ub2e4[1].
\n
\nTank buffer
\n \uc804\uae30\uc601\ub3d9\uc7a5\uce58\uc5d0 \ucc44\uc6b0\ub294 Tank buffer\uc5d0\ub294 glycine\uacfc Cl-(Tris-base)\uc774\uc628\uc774 \ub4e4\uc5b4\uac04\ub2e4. Glycine\uc740 \uc544\ubbf8\ub178\uc0b0\uc758 \uc131\uc9c8\ub85c \uc778\ud574 \uc74c\uc804\ud558\ub97c \ub760\uac70\ub098 net charge\uac00 0\uc778 \uc30d\uadf9\uc131 \uc774\uc628 \ud615\ud0dc\ub85c \uc874\uc7ac\ud55c\ub2e4. gel\uc5d0 \ud615\uc131\ub41c well\uc5d0 \ub2e8\ubc31\uc9c8 \uc2dc\ub8cc\ub97c \uac00\ud558\uace0 \uc804\uae30\uc601\ub3d9\uc744 \uc2dc\uc791\ud558\uba74 Cl-\uc774\uc628\uacfc \ub2e8\ubc31\uc9c8, Glycine \uc774\uc628\uc774 \ubaa8\ub450 (+)\uadf9\uc744 \ud5a5\ud574 \ucd9c\ubc1c\ud55c\ub2e4. \uadf8\ub7f0\ub370 glycine\uc740 stacking gel\uc758 \ub0ae\uc740 pH\uc5d0\uc11c \ub2e4\uc2dc \ud3c9\ud615\uc744 \uc774\ub8e8\uc5b4 \uc77c\ubd80\uac00 \uc30d\uadf9\uc131 \uc774\uc628\uc774 \ub418\uc5b4\ubc84\ub9ac\uae30 \ub54c\ubb38\uc5d0 \uc774 \ubd80\ubd84\uc5d0\uc11c \uc6c0\uc9c1\uc774\ub294 \uc774\uc628\uc774 \uc5c6\uc5b4\uc9c0\uace0, \uacb0\uacfc\uc801\uc73c\ub85c \uc804\ub958\uac00 \uac10\uc18c\ud55c\ub2e4. \uadf8\ub7ec\ub098, \uc804\uccb4 gel\uc758 \uc804\ub958\ub294 \uc720\uc9c0\ub418\uc5b4\uc57c \ud558\ubbc0\ub85c \uc774\ub97c \uadf9\ubcf5\ud558\uae30 \uc704\ud574\uc11c Cl-\uc774\uc628\uacfc glycine \uc0ac\uc774\uc5d0 \ub9e4\uc6b0 \ub192\uc740 \uc804\uc555\ucc28\uac00 \ud615\uc131\ub41c\ub2e4. \ub530\ub77c\uc11c, \uc0c1\ub300\uc801\uc778 \uc774\ub3d9\uc18d\ub3c4\ub294 glycine<\ub2e8\ubc31\uc9c8\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \u2160. Introduction \u2161. Materials & Methods \u2162. Result \u2163. Conclusion(Discussion) \u2164. Reference \ubcf8\ubb38\ub0b4\uc6a9 IPTG\ub780, Isopropyl-\u03b2-D-1-thiogalactopyranoside\uc758 \uc57d\uae30\ub85c \ub300\uc7a5\uade0 lactose operon\uc758 \ud6a8\uc18c\ud569\uc131\uc744 \uc720\ub3c4\ud558\ub294 \uac15\ub825\ud55c \uc720\ub3c4\ubb3c\uc9c8\uc774\ub2e4. \ubcf4\ud1b5 \uc720\ub3c4\ubb3c\uc9c8\uc740 operon\uc758 \ud65c\ub3d9\uc5d0 \uc758\ud574 \ub9cc\ub4e4\uc5b4\uc9c0\ub294 \u03b2-D-galactosidase\uc5d0 \uc758\ud574 \ub300\uc0ac \uc774\uc6a9\ud558\ub294 \uac83\uc774 \ub9ce\uc9c0\ub9cc, IPTG\ub294 \ubd84\ud574\ub418\uc9c0 \uc54a\uc544 \ube44\ub300\uc0ac\uc131 \uc720\ub3c4\ubb3c\uc9c8\uc774\ub77c\uace0\ub3c4 \ubd88\ub9b0\ub2e4. \ub610\ud55c, \uace0\ub18d\ub3c4\ub85c \uc0ac\uc6a9\uc2dc galactoside permease\uac00 \uc5c6\ub294 \uc138\ud3ec\uc5d0\ub3c4 \ud22c\uacfc\ud560 \uc218 \uc788\uae30 \ub54c\ubb38\uc5d0 permease\uac00 \uc5c6\ub294 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[33287,33288,33286],"class_list":["post-26638","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-iptg","tag-western-blot","tag-33286"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26638","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=26638"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26638\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=26638"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=26638"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=26638"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}