{"id":26641,"date":"2025-12-28T09:00:23","date_gmt":"2025-12-28T00:00:23","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/28\/26641\/"},"modified":"2025-12-28T09:00:23","modified_gmt":"2025-12-28T00:00:23","slug":"stenotrophomonas-maltophilia-%ec%9c%a0%eb%9e%98-oleate-hydratase%ec%99%80-alcohol-dehydrogenase%ec%9d%98-enzyme-cascade-reaction","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/28\/26641\/","title":{"rendered":"Stenotrophomonas maltophilia \uc720\ub798 Oleate Hydratase\uc640 Alcohol dehydrogenase\uc758 Enzyme cascade reaction"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\uc11c\ub860
\n2.\uc7ac\ub8cc \ubc0f \ubc29\ubc95
\n3.\uc2e4\ud5d8 \uacb0\uacfc
\n4.\uace0\ucc30 \ubc0f \uacb0\ub860
\n5.\ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uc9c0\ubc29\uc0b0 hydratase\ub294 unsaturated fatty acids\uc758 C-C \uc774\uc911\uacb0\ud569\uc744 \uc0b0\ud654\ud55c\ub2e4. \uadf8 \uc911 oleic acid\uc744 10-hydroxysteric acid\ub85c hydroxylation\uc744 \ucd09\ub9e4\ud558\ub294 oleate hydratase(Ohys)\ub294 \ube44\ub85d \ub0ae\uc740 \uc804\ud658\uc728\uc744 \uac00\uc9c0\uc9c0\ub9cc, \uccb4\uacc4\uc801\uc778 protein engineering \ubc0f substrate scope\uc758 \ubc94\uc704\ub97c \ub113\ud788\uace0 conversion rate\ub97c \ub192\ub2e4\ub294 \uc774\uc810\uc73c\ub85c \ucd5c\uadfc \uc0b0\uc5c5\uc801\uc73c\ub85c \ud2b9\ubcc4\ud55c \uad00\uc2ec\uc744 \ubc1b\uace0 \uc788\ub2e4.[1] \ubcf8 \uc2e4\ud5d8\uc5d0\uc11c\ub294 Ohys \ud6a8\uc18c \uacc4\uc5f4 \uc911 \ud558\ub098\uc778 Stenotrophomonas maltophilia oleate hydratase(SmOhyA)\uc758 enzymatic reaction\uc5d0 \ub300\ud55c kinetic \ubd84\uc11d\uc744 \uc9c4\ud589\ud558\uc600\ub2e4. SmOhyA\ub294 cofactor\ub85c FAD\ub97c \ud544\uc694\ub85c\ud558\ub294 FAD-dependent enzyme\uc774\uba70, unsaturated fatty acids\uc758 9-cis \uc704\uce58 \uc774\uc911\uacb0\ud569\uc5d0 \ub300\ud574\uc11c \uc120\ud0dd\uc801\uc73c\ub85c \uc218\ud654\ubc18\uc751\uc744 \uc77c\uc73c\ud0a4\ub294 regioselective hydration\uc744 \ucd09\ub9e4\ud55c\ub2e4. SmOhy\ub294 \ud2b9\ud788 oleic acid\uc5d0 \uac00\uc7a5 \ub192\uc740 \ud2b9\uc815 \ud65c\uc131\uc744 \uc9c0\ub2c8\uba70, polyunsaturated fatty acids(PUFAs)\uc5d0 \ub300\ud574\uc11c\ub294 \ub0ae\uc740 \ud65c\uc131\uc744 \ubcf4\uc778\ub2e4\ub294 \ud2b9\uc9d5\uc744 \uac00\uc9c0\uace0 \uc788\ub2e4. \ud6a8\uc18c \ub2e8\ubc31\uc9c8\uc758 \ud06c\uae30\ub294 \ub300\ub7b5 68kDa\ub85c \uce21\uc815\ub418\uba70, pH 6\uc778 \ud658\uacbd\uc5d0\uc11c \ucd5c\uc801\uc758 \ubc18\uc751\uc744 \ubcf4\uc778\ub2e4. SmOhy\uc758 \ud6a8\uc18c \ubc18\uc751 \uba54\ucee4\ub2c8\uc998\uc740 \uc544\uc9c1 \uad6c\uccb4\uc801\uc73c\ub85c \ubc1d\ud600\uc9c4 \ubc14\ub294 \uc5c6\uc9c0\ub9cc, \uac19\uc740 \ud6a8\uc18c \uacc4\uc5f4\uc758 Elizabethkingia meningoseptica Oleate Hydratase(EmOhy)\uc640 Staphylococcus aureus Oleate Hatratase(SaOhy)\uc758 \ubc18\uc751 \uba54\ucee4\ub2c8\uc998\uc744 \ud1b5\ud574 \uc720\ucd94\ud560 \uc218 \uc788\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1.\uc11c\ub860 2.\uc7ac\ub8cc \ubc0f \ubc29\ubc95 3.\uc2e4\ud5d8 \uacb0\uacfc 4.\uace0\ucc30 \ubc0f \uacb0\ub860 5.\ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \uc9c0\ubc29\uc0b0 hydratase\ub294 unsaturated fatty acids\uc758 C-C \uc774\uc911\uacb0\ud569\uc744 \uc0b0\ud654\ud55c\ub2e4. \uadf8 \uc911 oleic acid\uc744 10-hydroxysteric acid\ub85c hydroxylation\uc744 \ucd09\ub9e4\ud558\ub294 oleate hydratase(Ohys)\ub294 \ube44\ub85d \ub0ae\uc740 \uc804\ud658\uc728\uc744 \uac00\uc9c0\uc9c0\ub9cc, \uccb4\uacc4\uc801\uc778 protein engineering \ubc0f substrate scope\uc758 \ubc94\uc704\ub97c \ub113\ud788\uace0 conversion rate\ub97c \ub192\ub2e4\ub294 \uc774\uc810\uc73c\ub85c \ucd5c\uadfc \uc0b0\uc5c5\uc801\uc73c\ub85c \ud2b9\ubcc4\ud55c \uad00\uc2ec\uc744 \ubc1b\uace0 \uc788\ub2e4.[1] \ubcf8 \uc2e4\ud5d8\uc5d0\uc11c\ub294 Ohys […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,26690,33293],"class_list":["post-26641","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-26690","tag-33293"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26641","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=26641"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26641\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=26641"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=26641"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=26641"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}