{"id":26648,"date":"2025-12-28T09:00:31","date_gmt":"2025-12-28T00:00:31","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/28\/26648\/"},"modified":"2025-12-28T09:00:31","modified_gmt":"2025-12-28T00:00:31","slug":"%ec%84%b8%ed%8f%ac%ec%83%9d%eb%a6%ac%ed%95%99%ec%8b%a4%ed%97%98_%eb%8b%a8%eb%b0%b1%ec%a7%88-%ea%b2%80%ec%b6%9c_bca-assay-%eb%b7%b0%eb%a0%9b%eb%b0%98%ec%9d%91-%eb%8b%8c%ed%9e%88%eb%93%9c%eb%a6%b0","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/28\/26648\/","title":{"rendered":"\uc138\ud3ec\uc0dd\ub9ac\ud559\uc2e4\ud5d8_\ub2e8\ubc31\uc9c8 \uac80\ucd9c_BCA assay, \ubdf0\ub81b\ubc18\uc751, \ub2cc\ud788\ub4dc\ub9b0 \ubc18\uc751"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\u2160. \uae30\ubcf8\uc815\ubcf4
\n\u2161. \uc11c\ub860
\n\u2162. \uc2e4\ud5d8\uc7ac\ub8cc \ubc0f \ubc29\ubc95
\n\u2163. \uc2e4\ud5d8 \uacb0\uacfc \ubc0f \uacb0\ub860
\n\u2164. \uace0\ucc30
\n\u2165. \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\u2160. \uae30\ubcf8\uc815\ubcf4
\n\uc2e4\ud5d8 \uc81c\ubaa9: \ub2e8\ubc31\uc9c8 \uac80\ucd9c
\n\uc2e4\ud5d8 \ubaa9\uc801: \ub2e8\ubc31\uc9c8\uc5d0 \ub300\ud574\uc11c \uc54c\uace0, BCA assay, \ubdf0\ub81b\ubc18\uc751, \ub2cc\ud788\ub4dc\ub9b0 \ubc18\uc751\uc744 \ud1b5\ud574 \ub2e8\ubc31\uc9c8\uc744 \uac80\ucd9c\ud55c\ub2e4.<\/p>\n

\u2161. \uc11c\ub860
\n1. \ub2e8\ubc31\uc9c8(protein)
\n \ub2e8\ubc31\uc9c8\uc740 \uc544\ubbf8\ub178\uc0b0\uc774 \ud3a9\ud2f0\ub4dc \uacb0\ud569\uc73c\ub85c \uc5f0\uacb0\ub41c \ubcf5\uc7a1\ud55c \uc0ac\uc2ac\uad6c\uc870\uc758 \uace0\ubd84\uc790 \ubb3c\uc9c8\uc774\ub2e4. \uc544\ubbf8\ub178\uc0b0\uc740 \ud0c4\uc18c\uc5d0 \uc544\ubbf8\ub178\uae30(-NH2)\uc640 \uce74\ub974\ubcf5\uc2e4\uae30(-COOH)\uac00 \ubd99\uc5b4\uc788\ub294 \uac83\uc774 \ud2b9\uc9d5\uc774\ub2e4. \uc790\uc5f0\uacc4\uc758 20\uac00\uc9c0\uc758 \uc544\ubbf8\ub178\uc0b0\uc740 \uc544\ubbf8\ub178\uc0b0\uc774 \uac00\uc9c0\ub294 R\uae30\uc758 \uc885\ub958\uc5d0 \ub530\ub77c \uad6c\ubd84\ub41c\ub2e4. \ub2e8\ubc31\uc9c8\uc758 \uc874\uc7ac\ub97c \uc99d\uba85\ud558\uae30 \uc704\ud574\uc11c\ub294 \ubc1c\uc0c9\ubc18\uc751\uc744 \uc0ac\uc6a9\ud560 \uc218 \uc788\ub294\ub370, \ub2e8\ubc31\uc9c8 \ubd84\uc790\uc5d0 \ud3ec\ud568\ub41c \ubc18\uc751\uae30\uac00 \ud2b9\uc218\ud55c \uc2dc\uc57d\uacfc \uc5b4\uc6b8\ub824\uc11c \ub098\ud0c0\ub0b4\ub294 \ubc18\uc751\uc774\ub2e4(\uae40\uae30\ud0dc \uc678 4\uba85. 2009).<\/p>\n

2. BCA assay
\n \uc54c\uce7c\ub9ac\uc758 \uc870\uac74\uc5d0\uc11c \ub2e8\ubc31\uc9c8\uc758 \ud3a9\ud0c0\uc774\ub4dc \uacb0\ud569\uacfc \ubc18\uc751\ud558\uc5ec Cu2+\uac00 Cu+\ub85c \ud658\uc6d0\ub41c\ub2e4. \uc774\ud6c4 2\ubd84\uc790\uc758 Bicinchoninic acid(BCA)\uac00 \ud658\uc6d0\ub41c Cu+\uc640 \ubc30\uc704 \uacb0\ud569\ud574 562nm\uc5d0\uc11c \ucd5c\ub300 \ud761\uad11\ub3c4\ub97c \uac00\uc9c0\ub294 \uac15\ud55c \ubcf4\ub77c\uc0c9\uc744 \ub764\ub2e4. Cu+\uc758 \uc0dd\uc131\uc740 \uace7 \ub2e8\ubc31\uc9c8\uc758 \ub18d\ub3c4\uc640 \ube44\ub840\ud558\uae30 \ub54c\ubb38\uc5d0 \uc54c\ub824\uc9c0\uc9c0 \uc54a\uc740 \uc0d8\ud50c\uc758 \ub2e8\ubc31\uc9c8 \ud568\ub7c9\uc740 \uc54c\ub824\uc9c4 \ub2e8\ubc31\uc9c8 \ud45c\uc900\uacfc \ube44\uad50\ud558\uc5ec \ubd84\uad11\uad11\ub3c4\ubc95\uc73c\ub85c \uc54c\uc544\ub0bc \uc218 \uc788\ub2e4(Walker, J. M.. 2009).<\/p>\n

3. \ubdf0\ub81b\ubc18\uc751
\n \ubdf0\ub81b \uc2dc\uc57d\uc740 \ub2e8\ubc31\uc9c8\uacfc \ud3f4\ub9ac\ud3a9\ud2f0\ub4dc\ub97c \uac80\ucd9c\ud558\uae30 \uc704\ud574 \uc0ac\uc6a9\ud558\ub294 \uc2dc\uc57d\uc774\ub2e4. \ubdf0\ub81b\uc2dc\uc57d\uc740 \uc6d0\ub798 \ud478\ub978\uc0c9\uc744 \ub098\ud0c0\ub0b4\ub098, \ub2e8\ubc31\uc9c8\uacfc \ubc18\uc751\ud558\uba74 \uc790\uc8fc\uc0c9\uc744 \ub098\ud0c0\ub0b4\uace0 \uc9e7\uc740 \ud3f4\ub9ac\ud3a9\ud2f0\ub4dc\uc640 \ubc18\uc751\ud558\uba74 \ubd84\ud64d\uc0c9\uc73c\ub85c \ubcc0\ud55c\ub2e4(\uae40\uae30\ud0dc \uc678 4\uba85. 2009). \ubdf0\ub81b\uc740 \uc54c\uce7c\ub9ac\uc131 CuSO4(2\uac00\uc758 \uad6c\ub9ac\uc774\uc628)\uc640 \ubc18\uc751\ud558\uc5ec \ubcf4\ub77c\uc0c9\uc758 \ucc29\ud654\ud569\ubb3c\uc744 \ub9cc\ub4e0\ub2e4. \ub450 \uac1c \uc774\uc0c1\uc758 \ud3a9\ud2f0\ub4dc \uacb0\ud569\uc744 \uac00\uc9c4 \ud654\ud569\ubb3c\ub3c4 \ub9c8\ucc2c\uac00\uc9c0\ub85c \uc720\uc0ac\ud55c \ucc29\ud654\ud569\ubb3c\uc744 \ub9cc\ub4e4\uba70, \ub2e8\ubc31\uc9c8\uc758 \uacbd\uc6b0\uc5d0\ub294 \uccad\uc790\uc0c9 \ub610\ub294 \uc801\uc790\uc0c9\uc744 \ub098\ud0c0\ub0b8\ub2e4. \ucc29\ud654\ud569\ubb3c\uc758 \uc0c9\uc740 1-2\uc2dc\uac04 \uc815\ub3c4\ub294 \uc548\uc815\ud558\uac8c \uc720\uc9c0\ub418\ub098, \uadf8 \uc774\uc0c1\uc5d0\uc11c\ub294 \uc810\uc810 \uc0c9\ub3c4\uac00 \uc99d\uac00\ud55c\ub2e4(\uae40\uae30\ud0dc \uc678 4\uba85. 2009).<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \u2160. \uae30\ubcf8\uc815\ubcf4 \u2161. \uc11c\ub860 \u2162. \uc2e4\ud5d8\uc7ac\ub8cc \ubc0f \ubc29\ubc95 \u2163. \uc2e4\ud5d8 \uacb0\uacfc \ubc0f \uacb0\ub860 \u2164. \uace0\ucc30 \u2165. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \u2160. \uae30\ubcf8\uc815\ubcf4 \uc2e4\ud5d8 \uc81c\ubaa9: \ub2e8\ubc31\uc9c8 \uac80\ucd9c \uc2e4\ud5d8 \ubaa9\uc801: \ub2e8\ubc31\uc9c8\uc5d0 \ub300\ud574\uc11c \uc54c\uace0, BCA assay, \ubdf0\ub81b\ubc18\uc751, \ub2cc\ud788\ub4dc\ub9b0 \ubc18\uc751\uc744 \ud1b5\ud574 \ub2e8\ubc31\uc9c8\uc744 \uac80\ucd9c\ud55c\ub2e4. \u2161. \uc11c\ub860 1. \ub2e8\ubc31\uc9c8(protein) \ub2e8\ubc31\uc9c8\uc740 \uc544\ubbf8\ub178\uc0b0\uc774 \ud3a9\ud2f0\ub4dc \uacb0\ud569\uc73c\ub85c \uc5f0\uacb0\ub41c \ubcf5\uc7a1\ud55c \uc0ac\uc2ac\uad6c\uc870\uc758 \uace0\ubd84\uc790 \ubb3c\uc9c8\uc774\ub2e4. \uc544\ubbf8\ub178\uc0b0\uc740 \ud0c4\uc18c\uc5d0 \uc544\ubbf8\ub178\uae30(-NH2)\uc640 \uce74\ub974\ubcf5\uc2e4\uae30(-COOH)\uac00 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7376,19478,33303,33304],"class_list":["post-26648","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-bca-assay","tag-19478","tag-33303","tag-33304"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26648","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=26648"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26648\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=26648"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=26648"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=26648"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}