{"id":26844,"date":"2025-12-30T09:00:22","date_gmt":"2025-12-30T00:00:22","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/30\/26844\/"},"modified":"2025-12-30T09:00:22","modified_gmt":"2025-12-30T00:00:22","slug":"acetaminophen%ec%9d%98-uv-calibration-curve-%ec%9e%91%ec%84%b1","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/12\/30\/26844\/","title":{"rendered":"Acetaminophen\uc758 UV calibration curve \uc791\uc131"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Reagents
\n2. Apparatus
\n3. Procedure
\n4. Results
\n5. Discussion
\n6. References<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1.\tReagent
\n Acetaminophen, distilled water
\n1) Acetaminophen (paracetamol)
\n– Molecular Formula: C8H9NO2
\n– Molecular weight: 151.16 g\/mol
\n– Melting point: 168 \u2103
\n– Boiling point > 500 \u2103
\n– Solubility in water: 14mg\/ml at 25 \u2103<\/p>\n

2.\tApparatus
\nUV\/VIS spectrophotomer, beaker, volumetric flask, cuvette, pipette, scale, syringe filter<\/p>\n

3.\tProcedure
\n1)\tAcetaminophene 60 mg\/100 ml \ub18d\ub3c4\uc758 stock solution\uc744 \uc81c\uc870\ud558\uc600\ub2e4.
\n(\uc2e4\uc81c \uce21\uc815\ub41c acetaminophen\uc758 \uc9c8\ub7c9: 60.1 mg)
\n2)\t\uc6a9\uc561\uc5d0 \uc874\uc7ac\ud558\ub294 \uba3c\uc9c0 \ub4f1\uc758 \ubd88\uc21c\ubb3c\uc744 \uc81c\uac70\ud558\uae30 \uc704\ud558\uc5ec syringe filter (pore size: 0.45 um)\ub97c \uc774\uc6a9\ud558\uc5ec \ud55c\ubc88 \uac78\ub7ec\ub0b4\uc5c8\ub2e4.
\n3)\tStandard solution\uc744 \ub2e8\uacc4\ubcc4 \ud76c\uc11d(serial dilution)\ud558\uc5ec 50%, 25%, 12.5%, 6.25% solution\uc744 \uac01\uac01 4 ml\uc529 \uc81c\uc870\ud558\uc600\ub2e4.
\n4)\t\uc81c\uc870\ud55c solution\uc744 couvette\uc5d0 \ub2f4\uace0 UV spectrometer\ub97c \uc774\uc6a9\ud558\uc5ec \uac01\uac01\uc758 \ud761\uad11\ub3c4\ub97c \uce21\uc815(wave length: 254 nm)\ud55c \ub4a4, calibration curve\ub97c \uc791\uc131\ud558\uc600\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Reagents 2. Apparatus 3. Procedure 4. Results 5. Discussion 6. References \ubcf8\ubb38\ub0b4\uc6a9 1. Reagent Acetaminophen, distilled water 1) Acetaminophen (paracetamol) – Molecular Formula: C8H9NO2 – Molecular weight: 151.16 g\/mol – Melting point: 168 \u2103 – Boiling point > 500 \u2103 – Solubility in water: 14mg\/ml at 25 \u2103 2. Apparatus UV\/VIS spectrophotomer, beaker, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[33539,3137,33537,33538],"class_list":["post-26844","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-uv-calibration-curve","tag-3137","tag-33537","tag-33538"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=26844"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/26844\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=26844"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=26844"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=26844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}