{"id":28307,"date":"2026-01-13T09:01:48","date_gmt":"2026-01-13T00:01:48","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/13\/28307\/"},"modified":"2026-01-13T09:01:48","modified_gmt":"2026-01-13T00:01:48","slug":"enzyme-activity-colorimetric-of-catalase","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/13\/28307\/","title":{"rendered":"Enzyme Activity- Colorimetric of Catalase"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

I.\tIntroduction
\n1. Enzyme
\n2. Catalase
\n3. \ud761\uad11, \ud615\uad11, \ubc1c\uad11\uc758 \uc6d0\ub9ac
\n4. \ud45c\uc900 \uac80\uc815 \uace1\uc120\ubc95 (standard calibration curve methods)
\n5. \uc2e4\ud5d8 \uc6d0\ub9ac : \ub0a8\uc740 h2o2\uc591\uc744 \uac10\ubcc4\ud558\uc5ec catalase\ub18d\ub3c4 \uc778\uc9c0 \/ \uc90c\ud06c\ub85c\uc0b0\uc5fc+\uc544\uc138\ud2b8\uc0b0 -> cromatic acid
\n6. Dichromate\/Acetic acid (D\/A)<\/p>\n

II.\tMaterials<\/p>\n

III.\tMethod<\/p>\n

IV.\tResult<\/p>\n

V.\tDiscussion
\n1. 0.6unit\uacfc 0.8unit\uc758 \uacb0\uacfc\uac00 \ubc14\ub010 \ub4ef\ud55c \uc591\uc0c1\uc744 \ubcf4\uc784
\n2. sample 1, 2 \ub07c\ub9ac \uc720\uc758\ubbf8\ud55c \ucc28\uc774\uac00 \uc5c6\uc74c
\n3. \uc2dc\uc57d \uacc4\uc0b0 \uc6d0\ub9ac
\n4. \uc2e4\ud5d8 \uc911 \uc8fc\uc758 \uc0ac\ud56d<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Enzyme<\/p>\n

\ud6a8\uc18c(enzyme)\ub780 \uc0dd\uccb4 \ub0b4\uc758 \ud654\ud559\ubc18\uc751\uc744 \ub9e4\uac1c\ud558\ub294 \ub2e8\ubc31\uc9c8 \ucd09\ub9e4\uc774\ub2e4. \ud6a8\uc18c\uc640 \uacb0\ud569\ud558\ub294 \ud2b9\uc774\uc801 \ubc18\uc751\ubb3c\uc744 \uae30\uc9c8(substrate)\uc774\ub77c\uace0 \ud558\uace0 \uae30\uc9c8\uacfc \ubc18\uc751\ud558\uc5ec \ud65c\uc131\ud654 \uc5d0\ub108\uc9c0\ub97c \ub0ae\ucdb0 \ubc18\uc751\uc744 \ucd09\uc9c4 \uc2dc\ud0a8\ub2e4. \uae30\uc9c8\uc758 \uc785\uccb4\uad6c\uc870\uc640 \ud6a8\uc18c\uc758 \ud65c\uc131\ubd80\uc704\uac00 \ub9de\uc544\uc57c \uacb0\ud569\ud558\uc5ec \ubc18\uc751\uc774 \uc77c\uc5b4\ub098\uace0 \uc774\ub97c \uae30\uc9c8\ud2b9\uc774\uc131\uc744 \uac00\uc84c\ub2e4\uace0 \ud55c\ub2e4. \ud6a8\uc18c\ub294 \uacb0\uad6d \ub2e8\ubc31\uc9c8\uc774\ubbc0\ub85c 3\ucc28\uad6c\uc870\uc5d0 \uc911\uc694\ud55c \uc628\ub3c4, pH \ub4f1 \ud658\uacbd \uc870\uc120\uc774 \ud65c\uc131 \uc815\ub3c4\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce5c\ub2e4. <\/p>\n

\ucd09\ub9e4 \uc791\uc6a9\uc5d0\ub294 \ucd1d \ub124\uac00\uc9c0 \uc815\ub3c4\uc758 \ubc29\ubc95\uc774 \uc788\ub294\ub370 \ub2e4\uc74c\uacfc \uac19\ub2e4.
\n1) \ud2b9\uc774\uc801 \uc0b0-\uc5fc\uae30 \ucd09\ub9e4 \uc791\uc6a9 : \ubb3c\uc18d\uc5d0 \uc874\uc7ac\ud558\ub294 H+\ub610\ub294 OH-\uc774\uc628\ub9cc\uc744 \uc0ac\uc6a9\ud558\ub294 \ucd09\ub9e4
\n2) \uc77c\ubc18\uc801 \uc0b0-\uc5fc\uae30 \ucd09\ub9e4 \uc791\uc6a9 : \ub2e4\ub978 \ubd84\uc790\uac00 \uc591\uc131\uc790 \uc804\uc774\uc5d0 \uad00\uc5ec\ud558\uc5ec \uc791\uc6a9
\n3) \uacf5\uc720\uacb0\ud569\uc131 \ucd09\ub9e4 \uc791\uc6a9 : \ud6a8\uc18c\uc640 \uae30\uc9c8 \uc0ac\uc774 \uc77c\uc2dc\uc801\uc778 \uacf5\uc720 \uacb0\ud569\uc774 \ud615\uc131
\n4) \uae08\uc18d\uc774\uc628 \ucd09\ub9e4 \uc791\uc6a9 : \ud6a8\uc18c\uc5d0 \uacb0\ud569\ub41c \uae08\uc18d\uacfc \uae30\uc9c8 \uac04 \uc774\uc628\uc758 \uc0c1\ud638\uc791\uc6a9\uc744 \uc77c\uc73c\ud0b4 <\/p>\n

2. Catalase<\/p>\n

\uce74\ud0c8\ub808\uc774\uc2a4\ub294 O2 \ub300\uc0ac \ub3d9\uc548 \uc0dd\uc131\ub41c \uacfc\uc0b0\ud654\uc218\uc18c\uc5d0 \uc758\ud55c \uc0b0\ud654\uc801 \uc190\uc0c1\uc73c\ub85c\ubd80\ud130 \uc138\ud3ec\ub97c \ubcf4\ud638\ud558\ub294 \uae08\uc18d \ud6a8\uc18c\uc774\ub2e4. \uacfc\uc0b0\ud654\uc218\uc18c\ub97c \ubb3c\uacfc \uc0b0\uc18c\ub85c \ubd84\ud574\ud568\uc73c\ub85c\uc368 \uc138\ud3ec\ub97c \ubcf4\ud638\ud55c\ub2e4. \ubab8 \uc18d\uc5d0 \uc788\ub294 H2O2\ub294 \uccb4\ub0b4\uc5d0 \uc788\ub294 \ub2e4\ub978 \uae08\uc18d \uc774\uc628\uacfc \ubc18\uc751\ud558\uc5ec hydroxyl radical\uc744 \ub9cc\ub4e4 \uc218 \uc788\ub2e4. hydroxyl radical\uc740 \ubc18\uc751\uc131\uc774 \ub9e4\uc6b0 \ucee4\uc11c \uccb4\ub0b4\uc5d0\uc11c \uc21c\uac04\uc801\uc73c\ub85c \ubc18\uc751\ud560 \uc218 \uc788\ub2e4. \uc774\ub294 \uc548\uc815\ub41c \uc138\ud3ec\ub97c \uc190\uc0c1\uc2dc\ud0ac \uc218 \uc788\uae30\uc5d0 \uacfc\uc0b0\ud654\uc218\uc18c\ub97c \uccb4\ub0b4\uc5d0\uc11c \uc81c\uac70\ud558\ub294 \uac83\uc740 \ub9e4\uc6b0 \uc911\uc694\ud558\ub2e4\uace0 \ubcfc \uc218 \uc788\ub2e4. <\/p>\n

3. \ud761\uad11, \ud615\uad11, \ubc1c\uad11\uc758 \uc6d0\ub9ac
\n 1) \ud761\uad11(absorbance)<\/p>\n

\ud761\uad11\uc740 \uc0d8\ud50c\uc5d0 \uc758\ud574 \ud761\uc218\ub41c \ube5b\uc758 \uc138\uae30\ub97c \uce21\uc815\ud558\ub294 \ubc29\ubc95\uc774\ub2e4. \ud654\ud559\ubb3c\uc9c8\uc740 \uac01\uac01 \uace0\uc720\uc758 \uac12\uc73c\ub85c \uc798 \ud761\uc218\ud558\ub294 \ud30c\uc7a5\uc774 \uc788\ub2e4. \ub530\ub77c\uc11c \ud761\uc218\ub41c \ube5b\uc758 \uc138\uae30\ub97c \uce21\uc815\ud55c \ub2e4\uc74c \uc774\ub97c \ud1b5\ud574 \uc5b4\ub5a4 \ud654\ud569\ubb3c\uc774 \ub9ce\uc740\uc9c0 \uce21\uc815\ud560 \uc218 \uc788\ub2e4. <\/p>\n

2) \ud615\uad11(fluorescence)
\n \ubb3c\uc9c8\uc774 \ube5b\uc758 \uc790\uadf9\uc5d0 \uc758\ud558\uc5ec \uc5d0\ub108\uc9c0 \uc900\uc704\uac00 \uc62c\ub77c\uac14\ub2e4\uac00 \ub2e4\uc2dc \uace0\uc720\uc758 \uc5d0\ub108\uc9c0 \uc0c1\ud0dc\ub85c \ub3cc\uc544\uac00\uae30 \uc704\ud574 \ube5b\uc744 \ubc1c\uc0b0\ud558\ub294 \ud604\uc0c1\uc744 \ub9d0\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 I. Introduction 1. Enzyme 2. Catalase 3. \ud761\uad11, \ud615\uad11, \ubc1c\uad11\uc758 \uc6d0\ub9ac 4. \ud45c\uc900 \uac80\uc815 \uace1\uc120\ubc95 (standard calibration curve methods) 5. \uc2e4\ud5d8 \uc6d0\ub9ac : \ub0a8\uc740 h2o2\uc591\uc744 \uac10\ubcc4\ud558\uc5ec catalase\ub18d\ub3c4 \uc778\uc9c0 \/ \uc90c\ud06c\ub85c\uc0b0\uc5fc+\uc544\uc138\ud2b8\uc0b0 -> cromatic acid 6. Dichromate\/Acetic acid (D\/A) II. Materials III. Method IV. Result V. Discussion 1. 0.6unit\uacfc 0.8unit\uc758 \uacb0\uacfc\uac00 \ubc14\ub010 \ub4ef\ud55c \uc591\uc0c1\uc744 \ubcf4\uc784 2. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[35333,12093,19477,8836,1295],"class_list":["post-28307","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-catalase","tag-elisa","tag-19477","tag-8836","tag-1295"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/28307","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=28307"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/28307\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=28307"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=28307"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=28307"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}