{"id":28310,"date":"2026-01-13T09:01:52","date_gmt":"2026-01-13T00:01:52","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/13\/28310\/"},"modified":"2026-01-13T09:01:52","modified_gmt":"2026-01-13T00:01:52","slug":"%ec%83%9d%eb%aa%85%ea%b3%bc%ed%95%99%ec%8b%a4%ed%97%981-sds-page-coomassie-blue-staining","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/13\/28310\/","title":{"rendered":"\uc0dd\uba85\uacfc\ud559\uc2e4\ud5d81 SDS-PAGE & Coomassie blue Staining"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

I.\tIntroduction
\n1.\t SDS\uc758 \uc5ed\ud560 \ubc0f \uc6d0\ub9ac
\n2.\t Gel\uc5d0 \ub4e4\uc5b4\uac00\ub294 \uc2dc\ub8cc\uc758 \uc5ed\ud560
\n3.\t Resolving, stacking gel\uc758 \ucc28\uc774
\n4.\t Coomassie blue<\/p>\n

II.\tMaterials<\/p>\n

III.\tMethod<\/p>\n

IV.\tResult
\n1.\t Dokdo marker, gel \uc0ac\uc9c4
\n2.\t \uc815\uc81c\ud558\uace0\uc790 \ud588\ub358 \ub2e8\ubc31\uc9c8 AP\uc758 band \uc704\uce58 \ud45c\uc2dc
\n3.\t \uacb0\uacfc \uad00\ucc30 \ub0b4\uc6a9<\/p>\n

V.\tDiscussion
\n1.\t \uc815\uc81c \uc804\ubcf4\ub2e4 \uc815\uc81c \ud6c4 AP\uc758 \uc21c\ub3c4(\uc804\uccb4 \ub2e8\ubc31\uc9c8 \ubc34\ub4dc \uc911 AP band\uc758 intensity \ube44\uc728)\ub294 \ub192\uc544\uc84c\ub294\uac00? \uadf8\ub807\ub2e4\uba74, \uc5b4\ub5a4 \ub18d\ub3c4\uc758 \uc6a9\uc561\uc5d0\uc11c \uac00\uc7a5 \ub192\uc544\uc84c\ub294\uac00? \ub9cc\uc57d \uc815\uc81c \ud6c4 \uc21c\ub3c4\uac00 \ub192\uc544\uc9c0\uc9c0 \uc54a\uc558\ub2e4\uba74 \ubb34\uc5c7\uc774 \ubb38\uc81c\uc77c \uc218 \uc788\ub294\uac00?
\n2.\t \uc815\uc81c \ud558\uace0\uc790 \ud558\ub294 \ub2e8\ubc31\uc9c8 \uc678\uc758 band\uac00 \uc874\uc7ac\ud558\ub294 \uc774\uc720\ub294?<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. SDS\uc758 \uc5ed\ud560 \ubc0f \uc6d0\ub9ac<\/p>\n

SDS(sodium dodecyl sulfate)\ub294 \uacc4\uba74\ud65c\uc131\uc81c\uc758 \uc77c\uc885\uc73c\ub85c \ub2e8\ubc31\uc9c8\uc774\ub098 \ud575\uc0b0\uc758 \uad6c\uc870\ub97c \uae68\ub728\ub824 \uc120\ud615\uc73c\ub85c \ub9cc\ub4e4\uc5b4\uc8fc\ub294 \uc5ed\ud560\uc744 \ud55c\ub2e4. \uc544\ubbf8\ub178\uc0b0\uc758 \uc18c\uc218\uc131 \ubd80\ubd84\uacfc hydrophobic interaction\uc744 \ud558\uba70 \uacb0\ud569\ud558\ub294\ub370, \uc544\ubbf8\ub178\uc0b0 2\uac1c\ub2f9 1\uac1c \uc815\ub3c4 binding\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 \ubcc0\uc131\ud55c\ub2e4.<\/p>\n

2. Gel\uc5d0 \ub4e4\uc5b4\uac00\ub294 \uc2dc\ub8cc\uc758 \uc5ed\ud560<\/p>\n

\u2460 Tris-HCl: Stacking gel\uacfc Resolving gel\uc758 pH\ub97c \ub2e4\ub974\uac8c \ud558\uc5ec \uac01 gel\uc5d0\uc11c Glycine\uc758 charge \ub2e4\ub974\uac8c \uc870\uc815\ud574\uc900\ub2e4. \ub610\ud55c Cl-\ub97c \ucca8\uac00\ud574\uc8fc\uc5b4\uc11c \uc774\ud6c4 \uc804\uae30\uc601\ub3d9\uc5d0\uc11c \uc804\uae30\ub97c \uac78\uc5b4\uc904 \ub54c \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud558\uac8c\ub054 \ud574\uc900\ub2e4.
\n\u2461 SDS: \uc544\ubbf8\ub178\uc0b0 2\uac1c\uc5d0 \ud558\ub098\uc529 \uacb0\ud569\ud574 \ube44\uacf5\uc720 \uacb0\ud569\uc744 \uc800\ud574\uc2dc\ucf1c \ub2e8\ubc31\uc9c8\uc744 \ubcc0\uc131\uc2dc\ud0a4\uba70, \ubaa8\ub4e0 \ub2e8\ubc31\uc9c8\uc5d0 (-) masking\uc744 \uac78\uc5b4 \uc804\uadf9\uc744 \uac78\uc5c8\uc744 \ub54c \ub2e8\ubc31\uc9c8\ub4e4\uc774 (+)\ub97c \ud5a5\ud574 \uc774\ub3d9\ud560 \uc218 \uc788\uac8c \ud55c\ub2e4.
\n\u2462 APS: acrylamide\uc758 \uad50\ucc28\uc5f0\uacb0\uc744 \ucd09\ub9e4\ud558\uba70, \ubc18\uc751\uc758 Initiator\uc774\ub2e4. \ub530\ub77c\uc11c \ub77c\ub514\uce7c\uc758 \uac1c\uc2dc \ubb3c\uc9c8\uc774\uae30 \ub54c\ubb38\uc5d0 \uc2e4\ud5d8 \uc0ac\uc6a9\uc2dc \ub9cc\ub4e0\ub2e4.
\n\u2463 TEMED: APS\uc758 \uc911\uac04\uccb4\ub97c \uc548\uc815\ud654\uc2dc\ud0a4\ub294 Catalyst\uc774\ub2e4. free radical\uc744 \ud615\uc131\ud558\uac8c \ud568\uc73c\ub85c\uc368 gel\uc758 \ud615\uc131\uc744 \ucd09\ub9e4\ud55c\ub2e4.<\/p>\n

3. Resolving, stacking gel\uc758 \ucc28\uc774<\/p>\n

Resolving gel\uacfc Stacking gel\ub294 pH\uac00 \ub2e4\ub974\uace0, \uc774\uc5d0\ub530\ub77c \ub2e4\ub978 \uc5ed\ud560\uc744 \ud55c\ub2e4. \uba3c\uc800 stacking gel\uc740 \uc2dc\ub8cc\uac00 \uac19\uc740 \uc704\uce58\uc5d0\uc11c \uc804\uae30\uc601\ub3d9\uc744 \uc2dc\uc791\ud560 \uc218 \uc788\ub3c4\ub85d \ud55c\ub2e4. glycine \uc774\uc628\uc740 pl\uac12\uc774 6\uc73c\ub85c, pH\uac00 6.8\uc778 stacking gel\uc5d0\uc11c net charge\uac00 0\uc5d0 \uac00\uae5d\ub2e4. mobility\ub294 Cl\uc774\uc628, \ub2e8\ubc31\uc9c8, glycine\uc21c\uc11c\ub85c \ud06c\ub2e4. \ub530\ub77c\uc11c protein\uc774 glycine\uacfc Cl\uc774\uc628 \uc0ac\uc774\uc5d0 \uac07\ud600\uc788\uac8c\ub054 \ub9cc\ub4e0\ub2e4. \uc774 \uacfc\uc815\uc744 \ud1b5\ud574 \uc2dc\ub8cc\uac00 \uac19\uc740 \uc704\uce58\uc5d0\uc11c \uc804\uae30\uc601\ub3d9\uc744 \uc2dc\uc791 \ud560 \uc218 \uc788\ub3c4\ub85d \ud55c\ub2e4.
\n Resolving gel\uc740 pH\uac00 8.8\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 I. Introduction 1. SDS\uc758 \uc5ed\ud560 \ubc0f \uc6d0\ub9ac 2. Gel\uc5d0 \ub4e4\uc5b4\uac00\ub294 \uc2dc\ub8cc\uc758 \uc5ed\ud560 3. Resolving, stacking gel\uc758 \ucc28\uc774 4. Coomassie blue II. Materials III. Method IV. Result 1. Dokdo marker, gel \uc0ac\uc9c4 2. \uc815\uc81c\ud558\uace0\uc790 \ud588\ub358 \ub2e8\ubc31\uc9c8 AP\uc758 band \uc704\uce58 \ud45c\uc2dc 3. \uacb0\uacfc \uad00\ucc30 \ub0b4\uc6a9 V. Discussion 1. \uc815\uc81c \uc804\ubcf4\ub2e4 \uc815\uc81c \ud6c4 AP\uc758 \uc21c\ub3c4(\uc804\uccb4 \ub2e8\ubc31\uc9c8 \ubc34\ub4dc […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[35337,3798,1295],"class_list":["post-28310","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-coomassie-blue","tag-sds-page","tag-1295"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/28310","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=28310"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/28310\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=28310"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=28310"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=28310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}