{"id":29672,"date":"2026-01-27T09:00:47","date_gmt":"2026-01-27T00:00:47","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/27\/29672\/"},"modified":"2026-01-27T09:00:47","modified_gmt":"2026-01-27T00:00:47","slug":"%ec%b6%a9%eb%82%a8%eb%8c%80-%eb%b6%84%ec%9e%90%ec%83%9d%eb%ac%bc%ec%83%9d%ed%99%94%ed%95%99%ec%8b%a4%ed%97%98-%eb%8c%80%ec%9e%a5%ea%b7%a0-iptg-%ec%b2%98%eb%a6%ac-%ed%81%ac%eb%a1%9c%eb%a7%88","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/27\/29672\/","title":{"rendered":"[\ucda9\ub0a8\ub300] \ubd84\uc790\uc0dd\ubb3c\uc0dd\ud654\ud559\uc2e4\ud5d8 &#8211; \ub300\uc7a5\uade0 IPTG \ucc98\ub9ac, \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub798\ud53c, \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8 \uc0dd\uc0b0"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/04\/17\/data29732137-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumb212\/2024\/04\/17\/data29732137-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>I. \uc11c\ub860<\/p>\n<p>II. \uc7ac\ub8cc \ubc0f \ubc29\ubc95<br \/>\n  1. Recombinant protein production<br \/>\n  2. Total protein extraction<br \/>\n  3. Affinity chromatography (Ni-NTA)<br \/>\n  4. Size exclusion chromatography (FPLC)<br \/>\n  5. SDS-PAGE<br \/>\n  6. Bradford assay<br \/>\n  7. Enzyme activity assay<\/p>\n<p>III. \uacb0\uacfc<\/p>\n<p>IV. \ub17c\uc758<\/p>\n<p>V. \ucc38\uace0\ubb38\ud5cc<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p>Escherichia coli BL21 \uadf8\ub9ac\uace0 BL21(DE3)\uc740 \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8 \uc0dd\uc0b0\uc744 \uc704\ud55c \uc77c\ubc18\uc801\uc778 \uc2e4\ud5d8\uc2e4 \uade0\uc8fc\uc774\ub2e4. BL21\uc5d0\uc11c \ud30c\uc0dd\ub41c E.coli BL21(DE3)\uc740 \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8\uc758 \uace0\uc218\uc900 \ubc1c\ud604\uc5d0 \uac00\uc7a5 \ub110\ub9ac \uc0ac\uc6a9\ub418\uba70 T7 RNA polymerase \uc720\uc804\uc790\ub97c \uc81c\uc5b4\ud558\ub294 \ubc15\ud14c\ub9ac\uc624\ud30c\uc9c0\u03bb\uc5d0\uc11c \uc720\ub798\ub41c prophage DE3\ub97c \uac16\uace0 \uc788\ub2e4[1]. \ub300\uc7a5\uade0\uc5d0\uc11c \ub3c4\uc785 \uc720\uc804\uc790 \ubc1c\ud604\uc744 \uc704\ud574 \uc4f0\uc774\ub294 \ud504\ub85c\ubaa8\ud130\ub294 \uc8fc\ub85c \uc870\uc808\uc131\uc774\ub2e4. lac \ud504\ub85c\ubaa8\ud130\ub294 \ub300\uc7a5\uade0\uc758 \uc816\ub2f9 \uc624\ud398\ub860\uc5d0\uc11c \uc4f0\uc774\ub294 \ud504\ub85c\ubaa8\ud130\ub85c\uc11c lac repressor\uac00 \uc788\uc744 \ub54c \ubc1c\ud604\uc774 \uc5c6\ub2e4\uac00 \uc816\ub2f9 \ub610\ub294 \uc816\ub2f9\uc758 \uc720\uc0ac\uccb4(IPTG \ub4f1)\uac00 \uc788\uc744 \ub54c \uc5b5\uc81c\uc790\uac00 \ud504\ub85c\ubaa8\ud130\uc5d0 \uacb0\ud569\ud558\uc9c0 \ubabb\ud558\ubbc0\ub85c \uc720\uc804\uc790 \ubc1c\ud604\uc774 \uc77c\uc5b4\ub09c\ub2e4. \uc774 lac \ud504\ub85c\ubaa8\ud130\ub97c \ubc1c\ud604\ud558\uace0\uc790 \ud558\ub294 \ud45c\uc801\uc720\uc804\uc790\uc640 \ud568\uaed8 \uc0bd\uc785\ud558\uba74 \uc774 \uc720\uc804\uc790\ub294 \uc816\ub2f9 \uc758\uc874\uc801 \ubc1c\ud604\uc744 \ud558\uac8c \ub41c\ub2e4. T7 \ubc1c\ud604 \uc2dc\uc2a4\ud15c\uc740 lac \ud504\ub85c\ubaa8\ud130\uc640 T7 \ud504\ub85c\ubaa8\ud130\ub97c \ubcf5\ud569\uc801\uc73c\ub85c \uc0ac\uc6a9\ud558\ub294 \uac83\uc73c\ub85c \ub2e4\ub978 \ud504\ub85c\ubaa8\ud130\uc640 \ub2ec\ub9ac \ucd09\uc9c4\uc131 \ubc1c\ud604 \uc870\uc808 \uc591\uc2dd\uc744 \ucc44\ud0dd\ud558\uace0 \uc788\ub2e4. T7 \ubc1c\ud604 \uc2dc\uc2a4\ud15c\uc73c\ub85c \ub2e8\ubc31\uc9c8\uc744 \ubc1c\ud604\ud558\ub824\uba74 T7 \ud504\ub85c\ubaa8\ud130\ub97c \uc9c0\ub2cc \ubc1c\ud604 \ubca1\ud130\uc640 DE3 \uc720\uc804\uc790\uac00 \uc0bd\uc785\ub41c \ub300\uc7a5\uade0 \uade0\uc8fc\ub97c \uc0ac\uc6a9\ud574\uc57c \ud55c\ub2e4. DE3\ub780 lac \ud504\ub85c\ubaa8\ud130\uc5d0 \uc758\ud574 \uc870\uc808\ub418\ub294 T7 RNA \uc911\ud569\ud6a8\uc18c \uc720\uc804\uc790\uc774\ub2e4. T7 \ud504\ub85c\ubaa8\ud130\uac00 \ub4e0 \ubc1c\ud604 \ubca1\ud130\uc5d0 \ud45c\uc801\uc720\uc804\uc790\ub97c \uc7ac\uc870\ud569\ud558\uc5ec \uc801\uc808\ud55c \ub300\uc7a5\uade0 \uade0\uc8fc\uc5d0 \ub3c4\uc785\ud558\uc5ec \uc120\ubcc4\ud55c\ub2e4. \uc774\ud6c4 \uc774 \ub300\uc7a5\uade0\uc744 IPTG\ub85c \ucc98\ub9ac\ud558\uba74 T7 \uc911\ud569\ud6a8\uc18c\uc758 \ubc1c\ud604\uc774 \uc77c\uc5b4\ub09c\ub2e4. \uc774\ud6c4 \uc774 \uc911\ud569\ud6a8\uc18c\ub294 T7 \ud504\ub85c\ubaa8\ud130\uc5d0 \uacb0\ud569\ud558\uc5ec \ud45c\uc801\uc720\uc804\uc790 \ubc1c\ud604\uc744 \uc2dc\uc791\ud55c\ub2e4[2]. \uc2e4\ud5d8\uc5d0 \uc0ac\uc6a9\ud55c vector\ub294 pET28a\uc774\ub2e4. \uc720\ub3c4\ub418\uc9c0 \uc54a\uc740 \ubc1c\ud604\uc744 \uc5b5\uc81c\ud558\uae30 \uc704\ud574 T7 promoter \ubc0f \uc778\uc811\ud55c lac operator \uc11c\uc5f4\uc744 \ud3ec\ud568\ud55c\ub2e4. \ubc1c\ud604\ub420 coding \uc11c\uc5f4\uc740 poly-histidine purification \ubc0f thrombin protease recognition site\uc5d0 \ub300\ud55c \ucf54\ub529 \uc11c\uc5f4\uc758 frame\uacfc \ud568\uaed8 \ud074\ub85c\ub2dd\ub418\uc5b4 \uc0dd\uc0b0\ub41c \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8\uc744 \ub9cc\ub4e4 \uc218 \uc788\ub2e4[3].<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/29732137\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 I. \uc11c\ub860 II. \uc7ac\ub8cc \ubc0f \ubc29\ubc95 1. Recombinant protein production 2. Total protein extraction 3. Affinity chromatography (Ni-NTA) 4. Size exclusion chromatography (FPLC) 5. SDS-PAGE 6. Bradford assay 7. Enzyme activity assay III. \uacb0\uacfc IV. \ub17c\uc758 V. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 Escherichia coli BL21 \uadf8\ub9ac\uace0 BL21(DE3)\uc740 \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8 \uc0dd\uc0b0\uc744 \uc704\ud55c \uc77c\ubc18\uc801\uc778 \uc2e4\ud5d8\uc2e4 \uade0\uc8fc\uc774\ub2e4. BL21\uc5d0\uc11c \ud30c\uc0dd\ub41c E.coli [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[33286,36990,36988,36989],"class_list":["post-29672","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-33286","tag-36990","tag-36988","tag-36989"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/29672","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=29672"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/29672\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=29672"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=29672"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=29672"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}