{"id":29673,"date":"2026-01-27T09:00:47","date_gmt":"2026-01-27T00:00:47","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/27\/29673\/"},"modified":"2026-01-27T09:00:47","modified_gmt":"2026-01-27T00:00:47","slug":"%ec%b6%a9%eb%82%a8%eb%8c%80-%eb%b6%84%ec%9e%90%ec%83%9d%eb%ac%bc%ec%83%9d%ed%99%94%ed%95%99%ec%8b%a4%ed%97%98-%eb%8c%80%ec%9e%a5%ea%b7%a0%ec%97%90%ec%84%9c-%ec%9e%ac%ec%a1%b0%ed%95%a9-%eb%8b%a8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/01\/27\/29673\/","title":{"rendered":"[\ucda9\ub0a8\ub300] \ubd84\uc790\uc0dd\ubb3c\uc0dd\ud654\ud559\uc2e4\ud5d8 – \ub300\uc7a5\uade0\uc5d0\uc11c \uc7ac\uc870\ud569 \ub2e8\ubc31\uc9c8\uc758 \uc0dd\uc0b0\uacfc Western blot\uc5d0 \uad00\ud55c \uc2e4\ud5d8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

I. \uc11c\ub860
\nII. \uc7ac\ub8cc \ubc0f \ubc29\ubc95
\nIII. \uacb0\uacfc
\nIV. \ub17c\uc758
\nV. \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\ub300\uc7a5\uade0\uc5d0\uc11c \ub3c4\uc785 \uc720\uc804\uc790 \ubc1c\ud604\uc744 \uc704\ud574 \uc4f0\uc774\ub294 \ud504\ub85c\ubaa8\ud130\ub294 \uc8fc\ub85c \uc870\uc808\uc131\uc774\ub2e4. lac \ud504 \ub85c\ubaa8\ud130\ub294 \ub300\uc7a5\uade0\uc758 \uc816\ub2f9 \uc624\ud398\ub860\uc5d0\uc11c \uc4f0\uc774\ub294 \ud504\ub85c\ubaa8\ud130\ub85c\uc11c lac repressor\uac00 \uc788\uc744 \ub54c \ubc1c\ud604\uc774 \uc5c6\ub2e4\uac00 \uc816\ub2f9 \ub610\ub294 \uc816\ub2f9\uc758 \uc720\uc0ac\uccb4( IPTG \ub4f1)\uac00 \uc788\uc744 \ub54c \uc5b5\uc81c\uc790\uac00 \ud504\ub85c\ubaa8\ud130\uc5d0 \uacb0\ud569\ud558\uc9c0 \ubabb\ud558\ubbc0\ub85c \uc720\uc804\uc790 \ubc1c\ud604\uc774 \uc77c\uc5b4\ub09c\ub2e4. \uc774 lac \ud504\ub85c\ubaa8\ud130\ub97c \ubc1c\ud604\ud558\uace0\uc790 \ud558\ub294 \ud45c\uc801\uc720\uc804\uc790\uc640 \ud568\uaed8 \uc0bd\uc785\ud558\uba74 \uc774 \uc720\uc804\uc790\ub294 \uc816\ub2f9 \uc758\uc874\uc801 \ubc1c\ud604\uc744 \ud558\uac8c \ub41c\ub2e4. T7 \ubc1c\ud604 \uc2dc\uc2a4\ud15c\uc740 lac \ud504\ub85c\ubaa8\ud130\uc640 T7 \ud504\ub85c\ubaa8\ud130\ub97c \ubcf5\ud569\uc801\uc73c\ub85c \uc0ac\uc6a9\ud558\ub294 \uac83\uc73c\ub85c \ub2e4\ub978 \ud504\ub85c\ubaa8 \ud130\uc640 \ub2ec\ub9ac \ucd09\uc9c4\uc131 \ubc1c\ud604 \uc870\uc808 \uc591\uc2dd\uc744 \ucc44\ud0dd\ud558\uace0 \uc788\ub2e4. T7 \ubc1c\ud604 \uc2dc\uc2a4\ud15c\uc73c\ub85c \ub2e8\ubc31\uc9c8\uc744 \ubc1c\ud604\ud558\ub824\uba74 T7 \ud504\ub85c\ubaa8\ud130\ub97c \uc9c0\ub2cc \ubc1c\ud604 \ubca1\ud130\uc640 DE3 \uc720\uc804\uc790\uac00 \uc0bd\uc785\ub41c \ub300\uc7a5\uade0 \uade0\uc8fc\ub97c \uc0ac\uc6a9\ud574\uc57c \ud55c\ub2e4. DE3\ub780 lac \ud504\ub85c\ubaa8\ud130\uc5d0 \uc758\ud574 \uc870\uc808\ub418\ub294 T7 RNA \uc911\ud569\ud6a8\uc18c \uc720\uc804\uc790\uc774 \ub2e4. T7 \ud504\ub85c\ubaa8\ud130\uac00 \ub4e0 \ubc1c\ud604 \ubca1\ud130\uc5d0 \ud45c\uc801\uc720\uc804\uc790\ub97c \uc7ac\uc870\ud569\ud558\uc5ec \uc801\uc808\ud55c \ub300\uc7a5\uade0 \uade0\uc8fc \uc5d0 \ub3c4\uc785\ud558\uc5ec \uc120\ubcc4\ud55c\ub2e4. \uc774\ud6c4 \uc774 \ub300\uc7a5\uade0\uc744 IPTG\ub85c \ucc98\ub9ac\ud558\uba74 T7 \uc911\ud569\ud6a8\uc18c\uc758 \ubc1c\ud604\uc774 \uc77c\uc5b4\ub09c\ub2e4. \uc774\ud6c4 \uc774 \uc911\ud569\ud6a8\uc18c\ub294 T7 \ud504\ub85c\ubaa8\ud130\uc5d0 \uacb0\ud569\ud558\uc5ec \ud45c\uc801\uc720\uc804\uc790 \ubc1c\ud604\uc744 \uc2dc\uc791\ud55c \ub2e4[1].<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 I. \uc11c\ub860 II. \uc7ac\ub8cc \ubc0f \ubc29\ubc95 III. \uacb0\uacfc IV. \ub17c\uc758 V. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \ub300\uc7a5\uade0\uc5d0\uc11c \ub3c4\uc785 \uc720\uc804\uc790 \ubc1c\ud604\uc744 \uc704\ud574 \uc4f0\uc774\ub294 \ud504\ub85c\ubaa8\ud130\ub294 \uc8fc\ub85c \uc870\uc808\uc131\uc774\ub2e4. lac \ud504 \ub85c\ubaa8\ud130\ub294 \ub300\uc7a5\uade0\uc758 \uc816\ub2f9 \uc624\ud398\ub860\uc5d0\uc11c \uc4f0\uc774\ub294 \ud504\ub85c\ubaa8\ud130\ub85c\uc11c lac repressor\uac00 \uc788\uc744 \ub54c \ubc1c\ud604\uc774 \uc5c6\ub2e4\uac00 \uc816\ub2f9 \ub610\ub294 \uc816\ub2f9\uc758 \uc720\uc0ac\uccb4( IPTG \ub4f1)\uac00 \uc788\uc744 \ub54c \uc5b5\uc81c\uc790\uac00 \ud504\ub85c\ubaa8\ud130\uc5d0 \uacb0\ud569\ud558\uc9c0 \ubabb\ud558\ubbc0\ub85c \uc720\uc804\uc790 \ubc1c\ud604\uc774 \uc77c\uc5b4\ub09c\ub2e4. \uc774 lac \ud504\ub85c\ubaa8\ud130\ub97c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[33286,36990,36988,36989],"class_list":["post-29673","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-33286","tag-36990","tag-36988","tag-36989"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/29673","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=29673"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/29673\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=29673"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=29673"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=29673"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}