{"id":30559,"date":"2026-02-05T09:00:52","date_gmt":"2026-02-05T00:00:52","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/05\/30559\/"},"modified":"2026-02-05T09:00:52","modified_gmt":"2026-02-05T00:00:52","slug":"%ec%9d%b8%ec%b2%9c%eb%8c%80%ed%95%99%ea%b5%90-%eb%82%98%eb%85%b8%eb%b0%94%ec%9d%b4%ec%98%a4%ec%8b%a4%ed%97%981-genomic-dna-prep-gel-electrophoresis","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/05\/30559\/","title":{"rendered":"\uc778\ucc9c\ub300\ud559\uad50 \ub098\ub178\ubc14\uc774\uc624\uc2e4\ud5d8(1) Genomic DNA prep & Gel Electrophoresis"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\ubaa9\uc801
\n2. \uc774\ub860
\n3. \uc2e4\ud5d8\ubc29\ubc95 \ubc0f \uc8fc\uc758\uc0ac\ud56d
\n4. \uc2e4\ud5d8\uacb0\uacfc
\n5. \uace0\ucc30<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc774\ub860
\n– genomic DNA
\n: Genomic DNA\ub294 \uc5fc\uc0c9\uccb4 DNA\ub85c, Plasmid DNA\uc640 \uac19\uc740 Extra-chromosomal DNA\uc640 \ub300\uc870\ub418\ub294 \uac1c\ub150\uc774\ub2e4. \ub300\ubd80\ubd84\uc758 \uc0dd\ubb3c\uc740 \ubaa8\ub4e0 \uc138\ud3ec\uc5d0\uc11c \uac19\uc740 Genomic DNA\ub97c \uac16\uc9c0\ub9cc, \ud2b9\uc815 gene\uc758 \ubc1c\ud604\uc73c\ub85c cell type\uacfc function\uc774 \ub2ec\ub77c\uc9c4\ub2e4. \uc5b4\ub5a4 organism\uc758 genomic DNA\uc778\uc9c0\uc5d0 \ub530\ub77c \ub2e4\uc74c\uacfc \uac19\uc740 \ud2b9\uc131\uc744 \uc9c0\ub2cc\ub2e4.
\na. Genomic DNA of Virus
\nDNA \ubc14\uc774\ub7ec\uc2a4\uc758 \uc720\uc804\ubb3c\uc9c8\ub85c, \uc774\uc911 \uac00\ub2e5 \ub610\ub294 \ub2e8\uc77c \uac00\ub2e5 DNA\ub85c \uc774\ub8e8\uc5b4\uc838 \uc788\ub2e4. Linear \ud639\uc740 Circular\ud55c \uad6c\uc870\ub97c \uac00\uc9c0\uba70 \ub2e8\ubc31\uc9c8\ub85c \uc774\ub8e8\uc5b4\uc9c4 Capsid\ub77c\ub294 \uad6c\uc870\uc5d0 \ub458\ub7ec\uc2f8\uc5ec \uc788\ub2e4.
\nb. Genomic DNA of Prokaryotes<\/p>\n

<\uc911 \ub7b5><\/p>\n

1. \uace0\ucc30
\nMouse tail\uc5d0\uc11c genomic DNA\ub97c \ucd94\ucd9c\ud558\ub294 \uc2e4\ud5d8\uc744 \uc9c4\ud589\ud558\uc600\ub2e4. \uc774 \uc2e4\ud5d8\uc744 \ud1b5\ud574 genomic DNA\uc640 plasmid DNA\uc758 \ucc28\uc774\ub97c \uc54c\uace0, genomic DNA\ub97c \ubd84\ub9ac\ud558\uae30 \uc704\ud55c \ud654\ud559\uc801 \ubc29\ubc95\uacfc \ubb3c\ub9ac\uc801\ubc29\ubc95\uc758 \ud2b9\uc9d5\uc744 \uc54c \uc218 \uc788\ub2e4. genomic DNA\ub97c \ucd94\ucd9c\ud560 \uc218 \uc788\uace0, \uadf8 \uc6d0\ub9ac\ub97c \uc774\ud574\ud560 \uc218 \uc788\uc73c\uba70, nanodrop\ub97c \ud1b5\ud574 DNA \ub18d\ub3c4\ub97c \uce21\uc815\ud560 \uc218 \uc788\uace0, \uc804\uae30\uc601\ub3d9\uc73c\ub85c \ubd84\uc11d\ud560 \uc218 \uc788\ub2e4.
\ngenomic DNA\ub97c \ucd94\ucd9c\ud558\uae30 \uc704\ud574 Lysis, Precipation, Purification \uc138\ub2e8\uacc4\ub97c \uac70\uccd0 \uc9c4\ud589\ud55c\ub2e4. Lysis\uc5d0\uc11c\ub294 \uc138\ud3ec\uac00 \ubd84\ud574\ub418\uc5b4 DNA\ub97c \ubc29\ucd9c\ud558\uace0, \uc774 \uacfc\uc815\uc740 \uae30\uacc4\uc801\uc778 \ubd84\ud574 \ubc29\ubc95\uacfc \ud654\ud559\uc801 \ubd84\ud574 \ubc29\ubc95\uc774 \uc788\ub294\ub370 \ud654\ud559\uc801\uc73c\ub85c \ubd84\ud574\ud560 \ub54c\uc5d0\ub294 Proteinase K\uc640 \uac19\uc740 \ud6a8\uc18c\ub098 \uacc4\uba74\ud65c\uc131\uc81c\ub97c \uc0ac\uc6a9\ud574 \uc138\ud3ec \ub2e8\ubc31\uc9c8\uc744 \ubd84\ud574\uc2dc\ud0a8\ub2e4. Precipitation\uc5d0\uc11c\ub294 Cellular debris\ub85c\ubd80\ud130 free DNA\ub97c \ubd84\ub9ac\ud55c\ub2e4. \uc774\ub54c DNA \ubd84\uc790\uc758 \uc74c\uc804\ud558\ub97c \uc911\ud654\ud558\uae30 \uc704\ud574 \ub098\ud2b8\ub968 \uc774\uc628\uc744 \uc0ac\uc6a9\ud558\uace0, \uadf8 \ub2e4\uc74c Isopropanol\uacfc \uac19\uc740 Alcohol\uc774 \ucca8\uac00\ud558\uc5ec DNA\ub97c \uce68\uc804\uc2dc\ud0a8\ub2e4. \uc218\uc6a9\uc561\uc73c\ub85c\ubd80\ud130 DNA\ub97c \ubd84\ub9ac\ud55c \ud6c4…<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 2. \uc774\ub860 3. \uc2e4\ud5d8\ubc29\ubc95 \ubc0f \uc8fc\uc758\uc0ac\ud56d 4. \uc2e4\ud5d8\uacb0\uacfc 5. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 1. \uc774\ub860 – genomic DNA : Genomic DNA\ub294 \uc5fc\uc0c9\uccb4 DNA\ub85c, Plasmid DNA\uc640 \uac19\uc740 Extra-chromosomal DNA\uc640 \ub300\uc870\ub418\ub294 \uac1c\ub150\uc774\ub2e4. \ub300\ubd80\ubd84\uc758 \uc0dd\ubb3c\uc740 \ubaa8\ub4e0 \uc138\ud3ec\uc5d0\uc11c \uac19\uc740 Genomic DNA\ub97c \uac16\uc9c0\ub9cc, \ud2b9\uc815 gene\uc758 \ubc1c\ud604\uc73c\ub85c cell type\uacfc function\uc774 \ub2ec\ub77c\uc9c4\ub2e4. \uc5b4\ub5a4 organism\uc758 genomic DNA\uc778\uc9c0\uc5d0 \ub530\ub77c \ub2e4\uc74c\uacfc \uac19\uc740 \ud2b9\uc131\uc744 \uc9c0\ub2cc\ub2e4. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[16630,20278,20020,20021,8319,32928],"class_list":["post-30559","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-gel-electrophoresis","tag-genomic-dna-prep","tag-20020","tag-20021","tag-8319","tag-32928"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/30559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=30559"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/30559\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=30559"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=30559"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=30559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}