{"id":30886,"date":"2026-02-08T09:01:23","date_gmt":"2026-02-08T00:01:23","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/08\/30886\/"},"modified":"2026-02-08T09:01:23","modified_gmt":"2026-02-08T00:01:23","slug":"%ec%9c%a0%ea%b8%b0%ed%99%94%ed%95%99%ec%8b%a4%ed%97%98-a-%eb%a0%88%ed%8f%ac%ed%8a%b8_column-chromatography","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/08\/30886\/","title":{"rendered":"\uc720\uae30\ud654\ud559\uc2e4\ud5d8 A+ \ub808\ud3ec\ud2b8_Column chromatography"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title: Column chromatography
\n4. Principle & Object
\n5. Material
\n6. Procedure & Observation
\n7. Result
\n8. Discussion
\n9. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title: Column chromatography
\n2, 3\ubc88 \ud56d\ubaa9\uc758 \uacbd\uc6b0 \uc800\uc791\uc790 \uc2e4\uba85 \ubc0f \uc2e4\ud5d8 \ub0a0\uc9dc \uae30\uc7ac\ub85c \uc0ad\uc81c\ud558\uc600\uc2b5\ub2c8\ub2e4. 4. Principle & Object
\n4-1. Principle
\n1) Column Chromatography
\nColumn chromatography\ub294 \ud63c\ud569\ubb3c\ub85c\ubd80\ud130 \uc6d0\ud558\ub294 \ubb3c\uc9c8\uc744 mobile phase (eluent)\uc5d0\uc11c
\nstationary phase (column material)\uc5d0 \ub300\ud55c adsorption ability\uc758 \ucc28\uc774\ub97c \uc774\uc6a9\ud558\uc5ec
\n\ubd84\ub9ac\ud558\ub294 \uac83\uc774\ub2e4. Column chromatography\ub294 \uc561\uccb4\uc640 \uace0\uccb4\ub97c \ubd84\ub9ac\ud558\uac70\ub098 \uc815\uc81c\ud560 \ub54c \uac00\uc7a5 \uc720\uc6a9\ud55c \ubc29\ubc95 \uc911 \ud558\ub098\uc774\ub2e4. Eluent\ub97c column\uc5d0 \ud1b5\uacfc\uc2dc\ud0a4\uba74 \ud63c\ud569\ubb3c\uc5d0 \uc874\uc7ac\ud558\ub294 ingredient\ub4e4\uc774 stationary phase\ub97c \ub530\ub77c \uc6c0\uc9c1\uc774\ub294\ub370 \uac01 ingredient\uc758 stationary phase\uc5d0 \ub300\ud55c adsorption \uc815\ub3c4\uac00 \ub2e4\ub974\ub2e4. \ub530\ub77c\uc11c column\uc744 \ub098\uc624\ub294\ub370 \uac78\ub9ac\ub294 retention time\uc774 \uac01\uac01 \ub2e4\ub974\uace0, \uc774\ub7ec\ud55c \ucc28\uc774\ub97c \uc774\uc6a9\ud558\uc5ec \uc6d0\ud558\ub294 \uc131\ubd84 \ubb3c\uc9c8\uc744 \ubd84\ub9ac\ud560 \uc218 \uc788\ub2e4.
\n(1) Theories of Chromatography
\n\u2460 Retention\uc740 chromatographic system\ubb3c\uc9c8\uc774 \uc774\ub3d9\ud558\ub294 \uc18d\ub3c4\ub97c \uce21\uc815\ud55c \uac83\uc774\ub2e4. \ud654\ud569\ubb3c\uc774 eluent\uc73c\ub85c \uc6a9\ub9ac \ub418\ub294 HPLC \ub610\ub294 GC\uc640 \uac19\uc740 \uc5f0\uc18d \ud604\uc0c1 \uc2dc\uc2a4\ud15c\uc5d0\uc11c, Retention\uc740 \uc77c\ubc18\uc801\uc73c\ub85c Retention time Rt \ub610\ub294 tR, \uc8fc\uc785\uacfc \uac80\ucd9c \uc0ac\uc774\uc758 \uc2dc\uac04\uc73c\ub85c \uce21\uc815\ub41c\ub2e4. TLC\uc640 \uac19\uc740 interrupted development system\uc5d0\uc11c retention\uc740 \uc9c0\uc5f0 \uacc4\uc218 Rf\ub85c \uce21\uc815\ub418\uba70, \ud654\ud569\ubb3c\uc758 \uc774\ub3d9\uac70\ub9ac\ub97c eluent\uc758 \uc774\ub3d9\uac70\ub9ac\ub85c \ub098\ub208 \uac12\uc73c\ub85c \ub098\ud0c0\ub0b8\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title: Column chromatography 4. Principle & Object 5. Material 6. Procedure & Observation 7. Result 8. Discussion 9. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title: Column chromatography 2, 3\ubc88 \ud56d\ubaa9\uc758 \uacbd\uc6b0 \uc800\uc791\uc790 \uc2e4\uba85 \ubc0f \uc2e4\ud5d8 \ub0a0\uc9dc \uae30\uc7ac\ub85c \uc0ad\uc81c\ud558\uc600\uc2b5\ub2c8\ub2e4. 4. Principle & Object 4-1. Principle 1) Column Chromatography Column chromatography\ub294 \ud63c\ud569\ubb3c\ub85c\ubd80\ud130 \uc6d0\ud558\ub294 \ubb3c\uc9c8\uc744 mobile phase (eluent)\uc5d0\uc11c stationary […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9301,5276,11943,20400,11936],"class_list":["post-30886","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-__aa","tag-2022-","tag-chromatography","tag---tlc","tag-11936"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/30886","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=30886"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/30886\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=30886"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=30886"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=30886"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}