{"id":31427,"date":"2026-02-14T09:00:12","date_gmt":"2026-02-14T00:00:12","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/14\/31427\/"},"modified":"2026-02-14T09:00:12","modified_gmt":"2026-02-14T00:00:12","slug":"%ec%9d%bc%eb%b0%98%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%98%eb%8f%99%eb%ac%bc%ec%84%b8%ed%8f%ac%ec%9d%98-%ec%9c%a0%ec%82%ac%eb%b6%84%ec%97%b4-%ec%97%bc%ec%83%89%ec%b2%b4-%ea%b4%80%ec%b0%b0","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/14\/31427\/","title":{"rendered":"[\uc77c\ubc18\uc0dd\ubb3c\ud559\uc2e4\ud5d8]\ub3d9\ubb3c\uc138\ud3ec\uc758 \uc720\uc0ac\ubd84\uc5f4 \uc5fc\uc0c9\uccb4 \uad00\ucc30"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \ubaa9\uc801
\n2. \uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d
\n3. \uc2e4\ud5d8 \ubc29\ubc95
\n4. \uc2e4\ud5d8 \uacb0\uacfc
\n5. \ud1a0\uc758 \uc0ac\ud56d<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc2e4\ud5d8 \ubaa9\uc801
\n\uac00. \ub450 \uac00\uc9c0 \ubc29\ubc95\uc744 \ud1b5\ud574 \ub3d9\ubb3c\uc138\ud3ec\uc758 \uc720\uc0ac\ubd84\uc5f4 \uc5fc\uc0c9\uccb4\ub97c \uad00\ucc30\ud560 \uc218 \uc788\ub2e4.<\/p>\n

2. \uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d<\/p>\n

\uac00. \uc2e4\ud5d8 \uc7ac\ub8cc
\n1) Mouse cell(B16F10 cell, log phase of growth)
\n2) DMEM Medium containing 10% FBS and 1% penicilin
\n3) Cell culture dish, 50\u3396 conical tube, 1x PBS
\n4) Colchicine (10mg\/\u3396), 0.5% trypsin-EDTA, hypotonic solution (0.075M KCl)
\n5) Fixation solution (acetic acid 1 : methanol 3), Giemsa staining solution
\n6) Slide glass, cover glass, \ub77c\ud14d\uc2a4 \uc7a5\uac11, \ud734\uc9c0
\n7) 1\u3396 tip, 200\u3396 tip, 1\u3396 pipet, 200\u3395 pipet, TDW 100\u3396
\n8) \uc9d1\uac8c, \uad11\ud559\ud604\ubbf8\uacbd, DAPI chemical 100\u3395<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \ubaa9\uc801 2. \uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d 3. \uc2e4\ud5d8 \ubc29\ubc95 4. \uc2e4\ud5d8 \uacb0\uacfc 5. \ud1a0\uc758 \uc0ac\ud56d \ubcf8\ubb38\ub0b4\uc6a9 1. \uc2e4\ud5d8 \ubaa9\uc801 \uac00. \ub450 \uac00\uc9c0 \ubc29\ubc95\uc744 \ud1b5\ud574 \ub3d9\ubb3c\uc138\ud3ec\uc758 \uc720\uc0ac\ubd84\uc5f4 \uc5fc\uc0c9\uccb4\ub97c \uad00\ucc30\ud560 \uc218 \uc788\ub2e4. 2. \uc2e4\ud5d8 \uae30\uad6c \ubc0f \uc2dc\uc57d \uac00. \uc2e4\ud5d8 \uc7ac\ub8cc 1) Mouse cell(B16F10 cell, log phase of growth) 2) DMEM Medium containing 10% FBS […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7337,39299,39298,3799],"class_list":["post-31427","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-7337","tag-39299","tag-39298","tag-3799"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/31427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=31427"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/31427\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=31427"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=31427"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=31427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}