{"id":32477,"date":"2026-02-24T09:01:14","date_gmt":"2026-02-24T00:01:14","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/24\/32477\/"},"modified":"2026-02-24T09:01:14","modified_gmt":"2026-02-24T00:01:14","slug":"%ed%98%84%eb%8c%80%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%984-cycloheximide-chase-assay-full-report","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/02\/24\/32477\/","title":{"rendered":"\ud604\ub300\uc0dd\ubb3c\ud559\uc2e4\ud5d84 cycloheximide chase assay full report"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.Abstract
\n2.Introduction
\n3.Materials & Methods
\n4.Data & Results
\n5.Discussion
\n6.Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

Cycloheximide\ub294 \uc9c4\ud575\uc0c8\ud3ec \ub0b4\uc758 \ub2e8\ubc31\uc9c8 \ubc88\uc5ed\uacfc\uc815\uc5d0\uc11c \ub2e8\ubc31\uc9c8\uc744 \ud569\uc131\uc744 \uc5b5\uc81c\ud558\ub294 \ubb3c\uc9c8\uc774\ub2e4. Cycloheximide\ub97c \uc774\uc6a9\ud574 \ub2e8\ubc31\uc9c8 \ud569\uc131\uc744 \uc5b5\uc81c\ud55c \ud6c4 \uae30\uc874\uc5d0 \uc874\uc7ac\ud588\ub358 \ub2e8\ubc31\uc9c8\uc774 \ubd84\ud574\ub418\ub294 \uc2dc\uac04\uc744 \uce21\uc815\ud568\uc73c\ub85c\uc368 \ub2e8\ubc31\uc9c8 half-life\ub97c \uacb0\uc815\ud560 \uc218 \uc788\ub2e4. \ub2e8\ubc31\uc9c8\uc774 \ubd84\ud574\ub418\ub294 \uacfc\uc815\uc740 lysosome-autophagy pathway, ubiquitin-proteosome pathway \ub450\uac00\uc9c0\ub85c \ub098\ub20c \uc218 \uc788\ub2e4. Protein half-life\ub294 \ub2e8\ubc31\uc9c8\uc758 \uc138\ud3ec\uacfc\uc815\uc5d0\uc11c \ub2e8\ubc31\uc9c8 \ud48d\ubd80\ub3c4\ub97c \uacb0\uc815\ud558\uac70\ub098 \uc9c8\ubcd1\uc5d0 \ud2b9\uc774\uc801 \ub2e8\ubc31\uc9c8 degradation\uc744 \uc5f0\uad6c\ud558\ub294\ub370 \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud55c\ub2e4. \uc138\ud3ec\ub97c \ubc30\uc591\ud55c \ud6c4, \ub2e8\ubc31\uc9c8 \ub18d\ub3c4\ub97c \uacb0\uc815\ud558\uace0 \ub611\uac19\uc740 \uc591\uc758 \ub2e8\ubc31\uc9c8\uc744 \ucde8\ud55c\ub2e4. \uc774\ud6c4, SDS-page \u2013 transfer \u2013 western blotting \uacfc\uc815\uc744 \uac70\uccd0 \uacb0\uacfc\ub97c \ud655\uc778\ud55c\ub2e4. Western blotting \uacb0\uacfc\ub97c \ubc14\ud0d5\uc73c\ub85c image J \ud504\ub85c\uadf8\ub7a8\uc744 \uc774\uc6a9\ud574 \ub2e8\ubc31\uc9c8 \uc591\uc744 \uce21\uc815\ud558\uace0 \uc2dc\uac04\uc5d0 \ub530\ub77c \uac10\uc18c\ub41c \ub2e8\ubc31\uc9c8 \uc591\uc744 \ud655\uc778\ud574\ubcf8\ub2e4. cyclinD1\uacfc \uf061\uf062-tubulin \ub2e8\ubc31\uc9c8\ub7c9\uc758 \ubc31\ubd84\uc728\uc744 \uad6c\ud558\uc5ec \uc2dc\uac04 \uacbd\uacfc\uc5d0 \ub530\ub77c cyclin D1\uc758 \uc591\uc774 \uac10\uc18c\ud55c \uac83\uc744 \ud655\uc778\ud558\uc600\ub2e4. Cycloheximide\uc758 \uae30\uc804\uc744 \uc774\uc6a9\ud558\uc5ec protein-half life\ub97c \uacb0\uc815\ud560 \uc218 \uc788\ub294 \uc774\uc720\uc5d0 \ub300\ud574 \uc0dd\uac01\ud574\ubcf4\uc558\uace0 \ube44\uc2b7\ud55c \uc2e4\ud5d8\uc744 \uc9c4\ud589\ud55c \ub17c\ubb38 \uacb0\uacfc\uc640 \uc774\ubc88 \uc2e4\ud5d8\uc758 \uacb0\uacfc\ub97c \ube44\uad50\ud574\ubcf4\uace0 \ucc28\uc774\uac00 \ub098\ub294 \uc774\uc720\uc5d0 \ub300\ud574\uc11c\ub3c4 \uc0dd\uac01\ud574\ubcf4\uc558\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1.Abstract 2.Introduction 3.Materials & Methods 4.Data & Results 5.Discussion 6.Reference \ubcf8\ubb38\ub0b4\uc6a9 Cycloheximide\ub294 \uc9c4\ud575\uc0c8\ud3ec \ub0b4\uc758 \ub2e8\ubc31\uc9c8 \ubc88\uc5ed\uacfc\uc815\uc5d0\uc11c \ub2e8\ubc31\uc9c8\uc744 \ud569\uc131\uc744 \uc5b5\uc81c\ud558\ub294 \ubb3c\uc9c8\uc774\ub2e4. Cycloheximide\ub97c \uc774\uc6a9\ud574 \ub2e8\ubc31\uc9c8 \ud569\uc131\uc744 \uc5b5\uc81c\ud55c \ud6c4 \uae30\uc874\uc5d0 \uc874\uc7ac\ud588\ub358 \ub2e8\ubc31\uc9c8\uc774 \ubd84\ud574\ub418\ub294 \uc2dc\uac04\uc744 \uce21\uc815\ud568\uc73c\ub85c\uc368 \ub2e8\ubc31\uc9c8 half-life\ub97c \uacb0\uc815\ud560 \uc218 \uc788\ub2e4. \ub2e8\ubc31\uc9c8\uc774 \ubd84\ud574\ub418\ub294 \uacfc\uc815\uc740 lysosome-autophagy pathway, ubiquitin-proteosome pathway \ub450\uac00\uc9c0\ub85c \ub098\ub20c \uc218 \uc788\ub2e4. Protein half-life\ub294 \ub2e8\ubc31\uc9c8\uc758 \uc138\ud3ec\uacfc\uc815\uc5d0\uc11c \ub2e8\ubc31\uc9c8 \ud48d\ubd80\ub3c4\ub97c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[40399,7373,40400],"class_list":["post-32477","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cycloheximide","tag-protein-extraction","tag-40400"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/32477","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=32477"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/32477\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=32477"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=32477"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=32477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}