{"id":33254,"date":"2026-03-04T09:00:48","date_gmt":"2026-03-04T00:00:48","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/03\/04\/33254\/"},"modified":"2026-03-04T09:00:48","modified_gmt":"2026-03-04T00:00:48","slug":"%ed%98%84%eb%8c%80%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%984-wnt-b-catenin-signaling-activation-full-report","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/03\/04\/33254\/","title":{"rendered":"\ud604\ub300\uc0dd\ubb3c\ud559\uc2e4\ud5d84 wnt\/B-catenin signaling activation full report"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Abstract
\n2. Introduction
\n3. Materials & Methods
\n4. Data & Result
\n5. Discussion
\n6. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\ud615\uad11\ud604\ubbf8\uacbd\uc73c\ub85c transfection\uc758 \ud6a8\uc728\uc744 \ud655\uc778\ud558\uace0 B-catenin inhibition \uc778\uc790\ub97c inhibition \ud558\uc5ec western blot, image J\ub97c \uc774\uc6a9\ud574 B-catenin \ubc1c\ud604\ub7c9 \uc99d\uac00\ub97c \ud655\uc778\ud574 \ubcf8\ub2e4.
\n Transfection\uc740 \ub3d9\ubb3c\uc138\ud3ec \ub0b4\ub85c \uc678\ubd80 \uc720\uc804\ubb3c\uc9c8\uc744 \ub3c4\uc785\ud558\ub294 \uac83\uc744 \uc758\ubbf8\ud55c\ub2e4. Polymer, liposome \ub4f1\uc758 \ubb3c\uc9c8\uc744 \uc774\uc6a9\ud558\uc5ec DNA\uc640 membrane \uc0ac\uc774\uc758 \uc804\uae30\uc801 \ubc18\ubc1c\ub825\uc744 \uc5b5\uc81c\ud55c \ud6c4 \uc138\ud3ec \ub0b4\ub85c \ub3c4\uc785 \uc2dc\ud0ac \uc218 \uc788\ub2e4. \uc138\ud3ec\ub9c9\uc744 \ud30c\uad34\ud558\uc5ec \uc138\ud3ec\uc758 \uad6c\uc131\uc694\uc18c\ub97c \ub178\ucd9c\uc2dc\ucf1c Lysate\ub97c \uc5bb\ub294 \uacfc\uc815\uc744 cell lysis\ub77c\uace0 \ud55c\ub2e4. Bradford assay\ub294 \ub18d\ub3c4\ub97c \ubbf8\ub9ac \uc54c\uace0 \uc788\ub294 sample\uacfc \ud761\uad11\ub3c4 \uac04\uc758 \uad00\uacc4\uc2dd\uc744 \uc5bb\uc5b4\ub0b4 \ubbf8\uc9c0 \uc2dc\ub8cc\uc758 \ub18d\ub3c4\ub97c \uacb0\uc815\ud558\ub294 \uae30\ubc95\uc774\ub2e4. Western blot\uc740 \ud56d\uccb4\ub97c \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 \ubd84\uc11d, \uac80\ucd9c\ud574\ub0b4\ub294 \ubc29\ubc95\uc774\ub2e4. Cell\ub85c\ubd80\ud130 \uc5bb\uc740 \ub2e8\ubc31\uc9c8\uc744 size \ubcc4\ub85c \ubd84\ub9ac\ud558\uace0 membrane\uc5d0 \uc62e\uae34 \ud6c4 blocking\ud558\uc5ec \ud56d\uccb4\ub97c \ucc98\ub9ac\ud55c\ub2e4. 2\ucc28 \ud56d\uccb4\uc5d0 alkaline phosphatase(AP)\uac00 conjugation \ub418\uc5b4 \uc788\ub294\ub370 BCIP\/NBT Solution\uc744 \ub123\uc5b4\uc8fc\uba74 AP\uac00 \uc774\uc640 \ubc18\uc751\ud574 \uc0c9\uc18c\ub97c \ud615\uc131\ud55c\ub2e4. \uc774\ub85c \uc778\ud574, detection\uc774 \uac00\ub2a5\ud558\ub2e4.
\n \uba3c\uc800, HEK293T cell\uc744 \ubc30\uc591\ud558\uace0 Human \uf062-catenin(GFP-tagged) plasmid\ub97c HEK293T cell\uc5d0 transfection \ud55c\ub2e4. Cell lysis\ub97c \ud1b5\ud574 lysate\ub97c \uc5bb\uc5b4\ub0b4\uace0 Bradford assay\ub97c \uc774\uc6a9\ud574 \ub18d\ub3c4\ub97c \uacb0\uc815\ud55c\ub2e4. \uc774\ud6c4, SDS-PAGE\ub97c \uc9c4\ud589\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 size\ubcc4\ub85c \ubd84\ub9ac\ud574\ub0b8 \ud6c4 PVDF membrane\uc744 \uc774\uc6a9\ud574 transfer\uacfc\uc815\uc744 \uc9c4\ud589\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Abstract 2. Introduction 3. Materials & Methods 4. Data & Result 5. Discussion 6. Reference \ubcf8\ubb38\ub0b4\uc6a9 \ud615\uad11\ud604\ubbf8\uacbd\uc73c\ub85c transfection\uc758 \ud6a8\uc728\uc744 \ud655\uc778\ud558\uace0 B-catenin inhibition \uc778\uc790\ub97c inhibition \ud558\uc5ec western blot, image J\ub97c \uc774\uc6a9\ud574 B-catenin \ubc1c\ud604\ub7c9 \uc99d\uac00\ub97c \ud655\uc778\ud574 \ubcf8\ub2e4. Transfection\uc740 \ub3d9\ubb3c\uc138\ud3ec \ub0b4\ub85c \uc678\ubd80 \uc720\uc804\ubb3c\uc9c8\uc744 \ub3c4\uc785\ud558\ub294 \uac83\uc744 \uc758\ubbf8\ud55c\ub2e4. Polymer, liposome \ub4f1\uc758 \ubb3c\uc9c8\uc744 \uc774\uc6a9\ud558\uc5ec DNA\uc640 membrane \uc0ac\uc774\uc758 \uc804\uae30\uc801 \ubc18\ubc1c\ub825\uc744 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[41380,41379,40400],"class_list":["post-33254","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-b-catenin","tag-wnt-signaling","tag-40400"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/33254","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=33254"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/33254\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=33254"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=33254"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=33254"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}