{"id":33281,"date":"2026-03-04T09:01:15","date_gmt":"2026-03-04T00:01:15","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/03\/04\/33281\/"},"modified":"2026-03-04T09:01:15","modified_gmt":"2026-03-04T00:01:15","slug":"%ec%95%bd%ec%82%b0%ec%9d%98-%ed%95%b4%eb%a6%ac-%ec%83%81%ec%88%98-%ec%b8%a1%ec%a0%95-%ec%8b%a4%ed%97%98-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/03\/04\/33281\/","title":{"rendered":"\uc57d\uc0b0\uc758 \ud574\ub9ac \uc0c1\uc218 \uce21\uc815 \uc2e4\ud5d8 \uacb0\uacfc\ubcf4\uace0\uc11c"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\ubaa9\uc801 \ubc0f \uc11c\ub860
\n2. \ub370\uc774\ud130
\n3. \uace0\ucc30 \ubc0f \uacb0\ub860<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\u25ce \uc2e4\ud5d8\ubaa9\uc801 \ubc0f \uc11c\ub860
\n\ud37c\uc13c\ud2b8 \ub18d\ub3c4\uc640 \ubab0\ub18d\ub3c4\uc5d0 \uc54c\ub9de\uc740 \uc5ec\ub7ec \uc6a9\uc561\ub4e4\uc744 \uc81c\uc870\ud55c\ub2e4. \ub9cc\ub4e4\uc5b4\ub454 \uc6a9\uc561\ub4e4\uc744 \uc870\ud569\ud558\uc5ec pH\ub18d\ub3c4\uac00 \ub2e4\ub978 \uc5ec\ub7ec \uc0b0\uc131\ud615 \uc6a9\uc561\uacfc \uc5fc\uae30\uc131\ud615 \uc6a9\uc561\uc744 \ub9cc\ub4e4\uc5b4 UV-VIS Spectrophotometer\uc5d0 \ub123\uc5b4 \ud53c\ud06c \uac12\uc744 \uc77d\uc5b4 \ub4e4\uc778\ub2e4. \uc77d\uc5b4 \ub4e4\uc778 \ud53c\ud06c \uac12\ub4e4\uc744 \ud1b5\ud574 BTB\uc758 \ud574\ub9ac\uc0c1\uc218 pKa\uac12\uc744 Beer-Lambert \ubc95\uce59\uacfc Henderson-Hasselbalch \ubc29\uc815\uc2dd\uc744 \uc0ac\uc6a9\ud558\uc5ec \uad6c\ud558\ub3c4\ub85d \ud55c\ub2e4.<\/p>\n

\u25ce \ub370\uc774\ud130
\n** \ub4e4\uc5b4\uac00\uae30\uc5d0 \uc55e\uc11c, \uc6b0\ub9ac \uc870\uc758 \uc2e4\ud5d8\uc5d0\uc11c \ud761\uad11\ub3c4 \ub370\uc774\ud130\uac00 \uc81c\ub300\ub85c \uc798 \ub098\uc624\uc9c0 \ubabb\ud558\uc600\ub2e4. \uadf8\ub9ac\ud558\uc5ec \uc81c\uacf5\ub41c \ud761\uad11\ub3c4 \ub370\uc774\ud130\uc640 \ube44\uad50\ud574\ubcf4\uae30\ub85c \ud558\uc790.<\/p>\n

– 0.1M Na2CO3(aq) 6.9mL + BTB 0.1mL \ub85c \uc55e\uc758 \ub178\uc774\uc988\ub97c \ubb34\uc2dc\ud588\uc744 \ub54c \uac00\uc7a5 \ub192\uc740 \ud53c\ud06c\uac12 : 0.623(\uc2e4\ud5d8\uac12) \/ 0.113(\uc81c\uacf5\ub41c \uac12)<\/p>\n

<\uc911 \ub7b5><\/p>\n

\ud22c\uad11\ub3c4\ub294 \ub9e4\uc9c8\uc5d0 \uc758\ud55c \uc785\uc0ac \ubcf5\uc0ac\uc120\uc774 \ud22c\uacfc\ub418\ub294 \ubd84\uc728\ub85c, \ube5b\uc774 \uc5bc\ub9c8\ub098 \uc2dc\ub8cc\ub97c \uc798 \ud1b5\uacfc\ud558\ub294\uac00\uc5d0 \ub300\ud55c \ucc99\ub3c4\uc774\ub2e4. \ud22c\uad11\ub3c4\ub294 \ub2e4\uc74c\uacfc \uac19\uc740 \uc2dd\uc73c\ub85c \ub098\ud0c0\ub0b8\ub2e4.<\/p>\n

T=P\/P0 \u00b7\u00b7\u00b7\u00b7\u00b7 Eq.1 \ud22c\uad11\ub3c4 \uc2dd<\/p>\n

\ud761\uad11\ub3c4\ub294 \uc2dc\ub8cc\uac00 \uc5bc\ub9c8\ub098 \ube5b\uc744 \uc798 \ud761\uc218\ud558\ub294\uac00\ub97c \ub098\ud0c0\ub0b4\ub294 \ucc99\ub3c4\uc774\uba70, \ube5b\uc758 \uac10\uc18c\uc5d0 \ube44\ub840\ud55c\ub2e4. \ud761\uad11\ub3c4\ub294 \ub2e4\uc74c\uacfc \uac19\uc740 \uc2dd\uc73c\ub85c \ub098\ud0c0\ub0b8\ub2e4.
\nA=-log10T=logP0\/P \u00b7\u00b7\u00b7\u00b7\u00b7 Eq.2 \ud761\uad11\ub3c4 \uc2dd<\/p>\n

\ud22c\uad11\ub3c4\uac00 \ud074\uc218\ub85d \uc2dc\ub8cc\uc758 \ud761\uad11\ub3c4\ub294 \uc791\uc544\uc9c0\uba70, \ube5b\uc744 \uc798 \ud761\uc218\ud558\ub294 \ubb3c\uc9c8\uc77c\uc218\ub85d \ube5b\uc774 \uc798 \ud22c\uacfc\ud558\uc9c0 \ubabb\ud55c\ub2e4.<\/p>\n

\ube44\uc5b4-\ub78c\ubca0\ub974\ud2b8 \ubc95\uce59(Beer-Lambert Law)\uc740 \ub9e4\uc9c8\uc758 \uc131\uc9c8\uacfc \ube5b\uc758 \uac10\uc1e0\ud604\uc0c1\uc5d0 \ub300\ud55c \ubc95\uce59\uc774\ub2e4.
\n\ube44\uc5b4-\ub78c\ubca0\ub974\ud2b8 \ubc95\uce59\uc740 \ub2e4\uc74c\uc758 \uc2dd\uc73c\ub85c \ub098\ud0c0\ub0b8\ub2e4.
\nA = -log T = log (P0\/P) = abc = \u03b5bc \u00b7\u00b7\u00b7\u00b7\u00b7 Eq.3 Beer-Lambert Law [2]
\nA : \ud761\uad11\ub3c4 (absorbance)
\n\u03b5 : \ubab0\ud761\uad11\uacc4\uc218(molar absorptivity)
\nb : \uc140\uc758 \uae38\uc774 (cell distance)
\nC : \ub18d\ub3c4 (concentration)<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\ubaa9\uc801 \ubc0f \uc11c\ub860 2. \ub370\uc774\ud130 3. \uace0\ucc30 \ubc0f \uacb0\ub860 \ubcf8\ubb38\ub0b4\uc6a9 \u25ce \uc2e4\ud5d8\ubaa9\uc801 \ubc0f \uc11c\ub860 \ud37c\uc13c\ud2b8 \ub18d\ub3c4\uc640 \ubab0\ub18d\ub3c4\uc5d0 \uc54c\ub9de\uc740 \uc5ec\ub7ec \uc6a9\uc561\ub4e4\uc744 \uc81c\uc870\ud55c\ub2e4. \ub9cc\ub4e4\uc5b4\ub454 \uc6a9\uc561\ub4e4\uc744 \uc870\ud569\ud558\uc5ec pH\ub18d\ub3c4\uac00 \ub2e4\ub978 \uc5ec\ub7ec \uc0b0\uc131\ud615 \uc6a9\uc561\uacfc \uc5fc\uae30\uc131\ud615 \uc6a9\uc561\uc744 \ub9cc\ub4e4\uc5b4 UV-VIS Spectrophotometer\uc5d0 \ub123\uc5b4 \ud53c\ud06c \uac12\uc744 \uc77d\uc5b4 \ub4e4\uc778\ub2e4. \uc77d\uc5b4 \ub4e4\uc778 \ud53c\ud06c \uac12\ub4e4\uc744 \ud1b5\ud574 BTB\uc758 \ud574\ub9ac\uc0c1\uc218 pKa\uac12\uc744 Beer-Lambert \ubc95\uce59\uacfc Henderson-Hasselbalch \ubc29\uc815\uc2dd\uc744 \uc0ac\uc6a9\ud558\uc5ec \uad6c\ud558\ub3c4\ub85d […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18229,41398,41397,6369],"class_list":["post-33281","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-a--","tag-41398","tag-41397","tag-6369"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/33281","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=33281"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/33281\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=33281"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=33281"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=33281"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}