{"id":36338,"date":"2026-04-04T09:00:33","date_gmt":"2026-04-04T00:00:33","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/04\/36338\/"},"modified":"2026-04-04T09:00:33","modified_gmt":"2026-04-04T00:00:33","slug":"%ed%99%94%ea%b3%b5%ec%83%9d%eb%ac%bc%ea%b3%b5%ed%95%99-%eb%8b%a8%ec%9c%84%ec%a1%b0%ec%9e%91%ec%8b%a4%ed%97%981-enzyme-kinetic-assay-horseradish-peroxidase","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/04\/36338\/","title":{"rendered":"\ud654\uacf5\uc0dd\ubb3c\uacf5\ud559 \ub2e8\uc704\uc870\uc791\uc2e4\ud5d81 Enzyme kinetic assay : Horseradish Peroxidase"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\uc81c\ubaa9
\n2. \uc2e4\ud5d8\ub0a0\uc9dc
\n3. \uc2e4\ud5d8\ubaa9\uc801
\n4. \uc2e4\ud5d8 \uc774\ub860
\n5. \uc2e4\ud5d8\uae30\uad6c \ubc0f \uc2dc\uc57d
\n6. \uc2e4\ud5d8\ubc29\ubc95
\n7. \uc2e4\ud5d8\uacb0\uacfc
\n8. \uace0\ucc30
\n9. \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1.\uc2e4\ud5d8\uc81c\ubaa9: COD \uce21\uc815<\/p>\n

2.\uc2e4\ud5d8\ub0a0\uc9dc: 2021\/05\/17<\/p>\n

3.\uc2e4\ud5d8\ubaa9\uc801
\n\ubcf8 \uc2e4\ud5d8\uc5d0\uc11c\ub294 spectrophotometer\ub97c \uc774\uc6a9\ud558\uc5ec HRP(horseradish peroxidase)\uc5d0 \uc758\ud55c \uae30\uc9c8\uc758 \ubcc0\ud654\ub97c \uce21\uc815\ud558\uc5ec, \ud6a8\uc18c \ubc18\uc751\uc18d\ub3c4\ub860\uc758 \uae30\ubcf8\uc774 \ub418\ub294 Michaelis\u2013Menten \ubc29\uc815\uc2dd\uc744 \uc774\ud574\ud55c\ub2e4.<\/p>\n

4.\uc2e4\ud5d8 \uc774\ub860
\nMichaelis\u2013Menten \ubc29\uc815\uc2dd
\nMichaelis\u2013Menten kinetics\ub294 \uc0dd\ud654\ud559\ubd84\uc57c\uc5d0\uc11c \uac00\uc7a5 \uc798 \uc54c\ub824\uc9c4 \ud6a8\uc18c\uc640 \ubc18\uc751\uc18d\ub3c4\ub860\uc5d0 \uad00\ud55c \ubaa8\ub378 \uc911 \ud558\ub098\uc774\ub2e4.
\n[S] : \uae30\uc9c8(\ubc18\uc751\ubb3c, Substrate) S\uc758 \ub18d\ub3c4
\n[E] : \ud6a8\uc18c(Enzyme) E \uc758 \ub18d\ub3c4
\n[ES] : \ud6a8\uc18c-\uae30\uc9c8 \ubcf5\ud569\uccb4\uc758 \ub18d\ub3c4
\n[P] : \uc0dd\uc131\ubb3c(Product)\uc758 \ub18d\ub3c4
\nVmax : \ucd5c\ub300 \ubc18\uc751\uc18d\ub3c4 \uc0c1\uc218
\nKm : Michaelis \uc0c1\uc218
\n\uc774 \uc2dd\uc744 Michaelis\u2013Menten \uc2dd\uc774\ub77c\uace0 \ubd80\ub978\ub2e4. <\/p>\n

\ud6a8\uc18c \ubc18\uc751\uc18d\ub3c4\ub860(enzyme reaction kinetics)
\n\ub2e8\uc77c \uae30\uc9c8-\ud6a8\uc18c \ucd09\ub9e4\ubc18\uc751\uc758 \uc18d\ub3c4\uc2dd\uc740 Michaelis-Menten \uc18d\ub3c4\uc2dd\uc744 \uae30\ubcf8\uc73c\ub85c \ud55c\ub2e4. \uc0dd\ubb3c\uacc4\uc5d0\uc11c\ub294 \ub2e4\uae30\uc9c8-\ub2e4\ud6a8\uc18c \ubc18\uc751\uacfc \uac19\uc740 \ubcf4\ub2e4 \ubcf5\uc7a1\ud55c \ud6a8\uc18c-\uae30\uc9c8\uac04 \uc0c1\ud638\uc791\uc6a9\uc774 \uc77c\uc5b4\ub0a0 \uc218 \uc788\ub2e4. \ud6a8\uc18c\ub294 \uae30\uc9c8\uacfc \uacb0\ud569\ud560 \uc218 \uc788\ub294 \ud65c\uc131\ubd80\uc704\uc758 \uc22b\uc790\uac00 \uc77c\uc815\ud558\uae30 \ub54c\ubb38\uc5d0 \ub192\uc740 \uae30\uc9c8\ub18d\ub3c4\uc5d0\uc11c\ub294 \ud65c\uc131\ubd80\uc704 \ubaa8\ub450\uac00 \uae30\uc9c8\uacfc \uacb0\ud569\ud558\uc5ec \ud6a8\uc18c\uac00 \ud3ec\ud654\ub418\ub294 \uc0c1\ud0dc\uac00 \ub41c\ub2e4.
\n\ud6a8\uc18c \ubc18\uc751\uc18d\ub3c4\ub860\uc740 \ub2e4\uc74c\uacfc \uac19\uc774 \ud6a8\uc18c(E)-\uae30\uc9c8(S)\uc758 \ubcf5\ud569\uccb4[ES] \ud615\uc131\ub2e8\uacc4\uc758 \uac00\uc5ed\ubc18\uc751\uacfc ES \ubcf5\ud569\uccb4\uc758 \ube44\uac00\uc5ed \ud574\ub9ac\ub2e8\uacc4\uc758 2\ub2e8\uacc4\uc758 \ubc18\uc751\uccb4\uacc4\ub85c\ubd80\ud130 \uad6c\ud560 \uc218 \uc788\ub2e4.
\n\uc704 \ubc18\uc751\uccb4\uacc4\ub294 ES \ubcf5\ud569\uccb4\uac00 \ub2e4\uc18c \ube60\ub978 \uc18d\ub3c4\ub85c \ud615\uc131\ub418\uace0 \ub450\ubc88\uc9f8 \ub2e8\uacc4\uc758 \uc5ed\ubc18\uc751\uc18d\ub3c4\ub97c \ubb34\uc2dc\ud55c\ub2e4\ub294 \uac00\uc815\uc744 \uc804\uc81c\ub85c \ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\uc81c\ubaa9 2. \uc2e4\ud5d8\ub0a0\uc9dc 3. \uc2e4\ud5d8\ubaa9\uc801 4. \uc2e4\ud5d8 \uc774\ub860 5. \uc2e4\ud5d8\uae30\uad6c \ubc0f \uc2dc\uc57d 6. \uc2e4\ud5d8\ubc29\ubc95 7. \uc2e4\ud5d8\uacb0\uacfc 8. \uace0\ucc30 9. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 1.\uc2e4\ud5d8\uc81c\ubaa9: COD \uce21\uc815 2.\uc2e4\ud5d8\ub0a0\uc9dc: 2021\/05\/17 3.\uc2e4\ud5d8\ubaa9\uc801 \ubcf8 \uc2e4\ud5d8\uc5d0\uc11c\ub294 spectrophotometer\ub97c \uc774\uc6a9\ud558\uc5ec HRP(horseradish peroxidase)\uc5d0 \uc758\ud55c \uae30\uc9c8\uc758 \ubcc0\ud654\ub97c \uce21\uc815\ud558\uc5ec, \ud6a8\uc18c \ubc18\uc751\uc18d\ub3c4\ub860\uc758 \uae30\ubcf8\uc774 \ub418\ub294 Michaelis\u2013Menten \ubc29\uc815\uc2dd\uc744 \uc774\ud574\ud55c\ub2e4. 4.\uc2e4\ud5d8 \uc774\ub860 Michaelis\u2013Menten \ubc29\uc815\uc2dd Michaelis\u2013Menten kinetics\ub294 \uc0dd\ud654\ud559\ubd84\uc57c\uc5d0\uc11c \uac00\uc7a5 \uc798 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,19140,37440,12867,34413],"class_list":["post-36338","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-lg","tag-37440","tag-12867","tag-34413"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/36338","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=36338"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/36338\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=36338"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=36338"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=36338"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}