{"id":36342,"date":"2026-04-04T09:00:39","date_gmt":"2026-04-04T00:00:39","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/04\/36342\/"},"modified":"2026-04-04T09:00:39","modified_gmt":"2026-04-04T00:00:39","slug":"%ed%99%94%ea%b3%b5%ec%83%9d%eb%ac%bc%ea%b3%b5%ed%95%99-%eb%8b%a8%ec%9c%84%ec%a1%b0%ec%9e%91%ec%8b%a4%ed%97%981-%eb%8b%a8%eb%b0%b1%ec%a7%88-%ec%a0%95%eb%9f%89%eb%b2%95-%eb%b9%84%ea%b5%90","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/04\/36342\/","title":{"rendered":"\ud654\uacf5\uc0dd\ubb3c\uacf5\ud559 \ub2e8\uc704\uc870\uc791\uc2e4\ud5d81 \ub2e8\ubc31\uc9c8 \uc815\ub7c9\ubc95 \ube44\uad50"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\uc81c\ubaa9
\n2. \uc2e4\ud5d8\ub0a0\uc9dc
\n3. \uc2e4\ud5d8\ubaa9\uc801
\n4. \uc2e4\ud5d8\uc774\ub860
\n5. \uc2e4\ud5d8\uae30\uad6c \ubc0f \uc2dc\uc57d
\n6. \uc2e4\ud5d8\ubc29\ubc95
\n7. \uc2e4\ud5d8\uacb0\uacfc
\n8. \uace0\ucc30
\n9. \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1.\uc2e4\ud5d8\uc81c\ubaa9: \ub2e8\ubc31\uc9c8 \uc815\ub7c9\ubc95 \ube44\uad50<\/p>\n

2.\uc2e4\ud5d8\ub0a0\uc9dc: 2021\/05\/10<\/p>\n

3.\uc2e4\ud5d8\ubaa9\uc801
\nBicinchoninic Acid(BCA)\uc640 Bradford\ub97c \uc774\uc6a9\ud55c \ub2e8\ubc31\uc9c8 \uc815\ub7c9\ubc95\uc744 \uc774\uc6a9\ud558\uc5ec \ubbf8\uc9c0 \uc2dc\ub8cc\uc758 \ub2e8\ubc31\uc9c8 \ub18d\ub3c4\ub97c \uce21\uc815\ud558\uace0 \ub450 \ubc29\ubc95\uc758 \uacb0\uacfc\ub97c \ube44\uad50 \ubd84\uc11d\ud55c\ub2e4. \ub610\ud55c \ubbf8\uc9c0\uc2dc\ub8cc\ub97c \ubd84\uc11d\ud568\uc5d0 \uc788\uc5b4 \uc5b4\ub5a4 \ubc29\uc2dd\uc774 \uc801\uc808\ud55c\uc9c0 \ud310\ub2e8\ud55c\ub2e4.<\/p>\n

4.\uc2e4\ud5d8 \uc774\ub860
\nBCA Assay
\n1) \uc6d0\ub9ac
\n\ub2e8\ubc31\uc9c8\uc774 \uad6c\ub9ac \uc774\uc628(Cu2+)\uc744 1\uac00 \uc591\uc774\uc628(Cu1+)\uc73c\ub85c \ud658\uc6d0(reduction) \uc2dc\ud0ac \uc218 \uc788\ub294 \uc131\uc9c8\uc744 \uc774\uc6a9\ud55c\ub2e4. \ud658\uc6d0\ub41c \uad6c\ub9ac \uc774\uc628\uc740 Bicinchoninic Acid(BCA) \ubd84\uc790 2\uac1c\uc640 \ubc18\uc751\ud558\uc5ec \ubcf4\ub78f\ube5b\uc758 \ucc29\ubb3c\uc744 \ud615\uc131\ud558\uac8c \ub41c\ub2e4. \uc774 \ud654\ud569\ubb3c\uc740 \ud30c\uc7a5\uc774 562 nm\uc778 \ube5b \ud761\uc218\uc728\uc774 \ubc18\uc751 \uc804 BCA\ub098 \uad6c\ub9ac \uc774\uc628\uc774 \ub2f4\uae34 \uc6a9\uc561\uc5d0 \ube44\ud574\uc11c \ub192\uae30 \ub54c\ubb38\uc5d0 spectrophotometer\ub97c \uc0ac\uc6a9\ud574\uc11c \ud761\uad11\ub3c4 \ucc28\uc774\ub97c \uce21\uc815\ud560 \uc218 \uc788\ub2e4. \ubcf4\ub78f\ube5b \ucc29\ubb3c\uc774 \ub354 \ub9ce\uc774 \uc0dd\uc131\uc774 \ub418\uba74\uc11c \ud761\uad11\ub3c4 \uac12\uc774 \ucee4\uc9c0\uae30 \ub54c\ubb38\uc5d0 \ud761\uad11\ub3c4 \uac12\uacfc \ub2e8\ubc31\uc9c8 \uc591\uc740 \ube44\ub840\ud55c\ub2e4. <\/p>\n

2) \uc7a5\uc810
\n– \uc77c\ubc18\uc801\uc778 \ubc84\ud37c\ubb3c\uc9c8\uc5d0 \uac04\uc12d\uc744 \ub35c \ubc1b\ub294\ub2e4.
\n– \ub192\uc740 \uc628\ub3c4\uc5d0\uc11c \ud55c\ub2e4\uba74 \uc544\uc8fc \ubbfc\uac10\ud558\uace0 \ube60\ub978 \ubc18\uc751\uc744 \ud560 \uc218 \uc788\ub2e4.
\n– \uacc4\uba74\ud65c\uc131\uc81c\uc640 \uac19\uc774 \uc0ac\uc6a9\ud560 \uc218 \uc788\ub2e4.
\n– \ubc18\uc751 \uc2dc\uc57d\uc774 \uc548\uc815\uc801\uc774\ub2e4.
\n– \uc11c\ub85c \ub2e4\ub978 \ub2e8\ubc31\uc9c8\uac04\uc758 \ubc18\uc751 \ucc28\uc774\uac00 \uac70\uc758 \uc5c6\ub2e4.<\/p>\n

3) \ub2e8\uc810
\n– \uc2e4\uc628\uc5d0\uc11c \ubc18\uc751\uc774 \uc644\uc804\ud788 \uc218\ud589\ub418\uc9c0 \uc54a\ub294\ub2e4. (\ub2e4\ub7c9\uc758 \ub2e8\ubc31\uc9c8\uc744 \uc815\ub7c9\ud560 \ub54c \ubb38\uc81c)
\n– \ub18d\ucd95\ub41c \ub2e8\ubc31\uc9c8 \uc0d8\ud50c\uc740 \ud76c\uc11d\uc774 \uc885\uc885 \ud544\uc694\ud558\ub2e4.
\n– \ub2e8\ubc31\uc9c8\uc758 \uc131\uc9c8\uc774 \ubc14\ub010\ub2e4.<\/p>\n

Bradford Assay
\n1) \uc6d0\ub9ac
\n\uc5fc\uc0c9\uc57d\uc778 Coomassie brilliant blue dye \uac00 \ub2e8\ubc31\uc9c8\uacfc \uacb0\ud569\ud558\uba74 \ucd5c\ub300 \ud761\uad11 \ud30c\uc7a5\uc774 465nm\uc5d0\uc11c 595nm \ub85c \ubc14\ub00c\uac8c \ub418\ub294 \uc131\uc9c8\uc744 \uc774\uc6a9\ud558\uc5ec 595nm \uc5d0\uc11c\uc758 \ud761\uad11\ub7c9\uc774 \ub2e8\ubc31\uc9c8\uc758 \ub18d\ub3c4\ub97c \ub098\ud0c0\ub0b4\uac8c \ub418\ub294 \ubc29\ubc95\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\uc81c\ubaa9 2. \uc2e4\ud5d8\ub0a0\uc9dc 3. \uc2e4\ud5d8\ubaa9\uc801 4. \uc2e4\ud5d8\uc774\ub860 5. \uc2e4\ud5d8\uae30\uad6c \ubc0f \uc2dc\uc57d 6. \uc2e4\ud5d8\ubc29\ubc95 7. \uc2e4\ud5d8\uacb0\uacfc 8. \uace0\ucc30 9. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 1.\uc2e4\ud5d8\uc81c\ubaa9: \ub2e8\ubc31\uc9c8 \uc815\ub7c9\ubc95 \ube44\uad50 2.\uc2e4\ud5d8\ub0a0\uc9dc: 2021\/05\/10 3.\uc2e4\ud5d8\ubaa9\uc801 Bicinchoninic Acid(BCA)\uc640 Bradford\ub97c \uc774\uc6a9\ud55c \ub2e8\ubc31\uc9c8 \uc815\ub7c9\ubc95\uc744 \uc774\uc6a9\ud558\uc5ec \ubbf8\uc9c0 \uc2dc\ub8cc\uc758 \ub2e8\ubc31\uc9c8 \ub18d\ub3c4\ub97c \uce21\uc815\ud558\uace0 \ub450 \ubc29\ubc95\uc758 \uacb0\uacfc\ub97c \ube44\uad50 \ubd84\uc11d\ud55c\ub2e4. \ub610\ud55c \ubbf8\uc9c0\uc2dc\ub8cc\ub97c \ubd84\uc11d\ud568\uc5d0 \uc788\uc5b4 \uc5b4\ub5a4 \ubc29\uc2dd\uc774 \uc801\uc808\ud55c\uc9c0 \ud310\ub2e8\ud55c\ub2e4. 4.\uc2e4\ud5d8 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,19140,37440,12867,34413],"class_list":["post-36342","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-lg","tag-37440","tag-12867","tag-34413"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/36342","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=36342"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/36342\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=36342"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=36342"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=36342"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}