{"id":36359,"date":"2026-04-04T09:01:02","date_gmt":"2026-04-04T00:01:02","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/04\/36359\/"},"modified":"2026-04-04T09:01:02","modified_gmt":"2026-04-04T00:01:02","slug":"pcr-%ea%b8%b0%eb%b0%98-specific-human-dna-%ec%a6%9d%ed%8f%ad-%eb%b0%8f-%ea%b2%80%ec%b6%9c-%ea%b2%b0%ea%b3%bc-%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/04\/36359\/","title":{"rendered":"PCR \uae30\ubc18 Specific Human DNA \uc99d\ud3ed \ubc0f \uac80\ucd9c \uacb0\uacfc \ubcf4\uace0\uc11c"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc81c\ubaa9
\n2. \uc2e4\ud5d8 \ubc29\ubc95
\n3. \uc2e4\ud5d8 \uacb0\uacfc
\n4. \uace0\ucc30
\n5. \uacb0\ub860
\n6. \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc2e4\ud5d8 \uc81c\ubaa9
\nPCR \uae30\ubc18 Specific Human DNA \uc99d\ud3ed \ubc0f \uac80\ucd9c<\/p>\n

2. \uc2e4\ud5d8 \ubc29\ubc95
\n\u2460 1 mL\uc758 protease solution (1\/1000\ub85c \ud76c\uc11d\ub41c)\uc744 E.P tube\uc5d0 \ub123\ub294\ub2e4.
\n\u2461 \uba74\ubd09\uc744 \uc0ac\uc6a9\ud574 \uad6c\uac15 \ucc9c\uc7a5\uc744 \uae01\uc5b4 \uc138\ud3ec\ub97c \ubaa8\uc740\ub2e4.
\n\u2462 \uba74\ubd09 \ub05d\uc5d0 \ubaa8\uc544\uc9c4 \uc138\ud3ec\ub97c protease solution\uc5d0 \ub123\ub294\ub2e4.
\n(\uba74\ubd09\uc744 \uc6b0\uce21 \uadf8\ub9bc\uacfc \uac19\uc774 \uc9dc\uc11c \uc6a9\uc561\uc744 \ucd5c\ub300\ud55c \ubaa8\uc544\uc900\ub2e4.)
\n\u2463 Solution\uc744 37\u2103\uc5d0\uc11c 30\ubd84\uac04 incubation \ud55c\ub2e4.
\n\u2464 4\uac1c\uc758 EP tube\uc5d0 200\u03bcL\uc529 \ub098\ub220 \ub2f4\ub294\ub2e4.
\n\u2465 20 \u03bcL\uc758 sodium acetate\uc640 500 \u03bcL\uc758 100% Ethanol\uc744
\n\u2464\uc5d0 \uc900\ube44\ub41c \uc6a9\uc561\uc5d0 \ucca8\uac00\ud55c\ub2e4.
\n\u2466 Vortexing \ud6c4 -80\u2103\uc5d0\uc11c 15\ubd84\uac04 \ub0c9\uac01\uc2dc\ud0a8\ub2e4.
\n\u2467 15,000 rpm, 4\u2103\uc5d0\uc11c 15\ubd84\uac04 centrifuge\ud55c\ub2e4.
\n\u2468 \uc0c1\uce35\uc561\uc744 \uc81c\uac70\ud55c \ub4a4 1mL\uc758 70% ethanol\uc744 \ub123\ub294\ub2e4.
\n\u2469 15,000 rpm, 4\u2103\uc5d0\uc11c 2\ubd84\uac04 centrifuge\ud55c\ub2e4.
\n\u246a \uc0c1\uce35\uc561\uc744 \uc81c\uac70\ud55c \ub4a4 \uce68\ucd9c\ub41c DNA\ub97c 15\ubd84\uac04 \uc0c1\uc628\uc5d0\uc11c \uac74\uc870\uc2dc\ud0a8\ub2e4.
\n\u246b 100\u03bcL\uc758 water\ub85c tube(1)\uc5d0\ub9cc \ub123\uc5b4 DNA\ub97c \ud480\uc5b4\uc900 \ub4a4, tube(1)\uc758 \uc6a9\uc561\uc744 \ub2e4\uc74c \ud29c \ube0c\ub85c \uc62e\uaca8\uac00\uba70 (tube(1)\u2192tube(2)\u2192tube(3)\u2192tube(4)) \ub098\uba38\uc9c0 tube\uc758 DNA\ub97c \ud480\uc5b4 \ud558 \ub098\ub85c \ub9cc\ub4e0 \ub4a4 vortexing \ud574\uc900\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \uc81c\ubaa9 2. \uc2e4\ud5d8 \ubc29\ubc95 3. \uc2e4\ud5d8 \uacb0\uacfc 4. \uace0\ucc30 5. \uacb0\ub860 6. \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 1. \uc2e4\ud5d8 \uc81c\ubaa9 PCR \uae30\ubc18 Specific Human DNA \uc99d\ud3ed \ubc0f \uac80\ucd9c 2. \uc2e4\ud5d8 \ubc29\ubc95 \u2460 1 mL\uc758 protease solution (1\/1000\ub85c \ud76c\uc11d\ub41c)\uc744 E.P tube\uc5d0 \ub123\ub294\ub2e4. \u2461 \uba74\ubd09\uc744 \uc0ac\uc6a9\ud574 \uad6c\uac15 \ucc9c\uc7a5\uc744 \uae01\uc5b4 \uc138\ud3ec\ub97c \ubaa8\uc740\ub2e4. \u2462 \uba74\ubd09 \ub05d\uc5d0 \ubaa8\uc544\uc9c4 \uc138\ud3ec\ub97c protease […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,42517,37440,12867,34413],"class_list":["post-36359","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-cpcr","tag-37440","tag-12867","tag-34413"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/36359","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=36359"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/36359\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=36359"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=36359"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=36359"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}