{"id":38967,"date":"2026-04-30T09:01:03","date_gmt":"2026-04-30T00:01:03","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/30\/38967\/"},"modified":"2026-04-30T09:01:03","modified_gmt":"2026-04-30T00:01:03","slug":"%ec%97%ad%ec%83%81-hplc-uv%eb%a5%bc-%ec%9d%b4%ec%9a%a9%ed%95%9c-indomethacin-%eb%b6%84%ec%84%9d-%ed%8a%b9%ec%9d%b4%ec%84%b1%ea%b3%bc-%ec%a7%81%ec%84%a0%ec%84%b1-%eb%b6%84%ec%84%9d-%eb%b0%8f","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/04\/30\/38967\/","title":{"rendered":"\uc5ed\uc0c1 HPLC\/UV\ub97c \uc774\uc6a9\ud55c indomethacin \ubd84\uc11d. \ud2b9\uc774\uc131\uacfc \uc9c1\uc120\uc131 \ubd84\uc11d \ubc0f \ubbf8\uc9c0\ub18d\ub3c4 \uad6c\ud558\uae30"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc81c\ubaa9 & \uc2e4\ud5d8 \ubaa9\uc801
\n2. \ubc29\ubc95
\n3. \uacb0\uacfc
\n4. \uace0\ucc30<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc81c\ubaa9 & \uc2e4\ud5d8 \ubaa9\uc801
\n \uc5ed\uc0c1 HPLC\/UV\ub97c \uc774\uc6a9\ud55c indomethacin \ubd84\uc11d. \ud2b9\uc774\uc131\uacfc \uc9c1\uc120\uc131\uc744 \ubd84\uc11d\ud558\uace0 \ubbf8\uc9c0\ub18d\ub3c4 \uad6c\ud558\uae30
\n\uc57d\ubb3c peak\uc640 retention time\uc744 \ud1b5\ud574 indomethacin\uacfc docetaxel \uc758 \ud2b9\uc774\uc131\uc744 \ud655\uc778\ud558\uace0, \uc0d8\ud50c\uc744 \uacc4\uc5f4 \ud76c\uc11d\uc2dc\ucf1c \uc9c1\uc120\uc131\uc744 \uad6c\ud558\uace0 \ubbf8\uc9c0 \uc57d\ubb3c\uc758 \ub18d\ub3c4\ub97c \uad6c\ud558\ub294 \uac83\uc774 \uc2e4\ud5d8\uc758 \ubaa9\uc801\uc774\ub2e4.<\/p>\n

2. \ubc29\ubc95
\n– Standard samples\uc758 \uc900\ube44
\nIndomethacin \uc6d0\uc561(stock solution, 1 mg\/mL)\uc744 \ud76c\uc11d\uc6a9\ub9e4 100% acetonitrile\ub97c \uc774\uc6a9\ud558\uc5ec \uc801\uc808\ud788 \uacc4\uc5f4 \ud76c\uc11d\ud558\uc5ec 2, 5, 10, 20, 50, 100 \u03bcg\/mL \ub18d\ub3c4\ub97c \uc900\ube44\ud588\ub2e4. <\ud45c1><\/p>\n

<\ud45c1> \uacc4\uc5f4\ud76c\uc11d \ubc29\ubc95 (\uc21c\uc11c\ub294 \uc67c\ucabd\ubd80\ud130 \uc624\ub978\ucabd\uc73c\ub85c)
\n\t100 ug\/ml\t50 ug\/ml\t20 ug\/ml\t10 ug\/ml\t5 ug\/ml\t2 ug\/ml
\nIND or \uc804\ub2e8\uacc4 \uacc4\uc5f4\ud76c\uc11d\ud55c IND(uL)\t10\t50\t40\t50\t50\t40
\nACN(uL)\t90\t50\t60\t50\t50\t60<\/p>\n

\uc774 standard samples\uc740 \ub2e4\uc74c \ub2e8\uacc4\uc5d0\uc11c HPLC \ubd84\uc11d\uc6a9 samples (drug in rat plasma)\uc744 \ub9cc\ub4e4 \ub54c \uc0ac\uc6a9\ub418\uc5c8\ub2e4. Internal standard\ub85c \uc0ac\uc6a9\ud558\ub294 docetaxel(1 mg\/mL)\uc740 \uc0ac\uc6a9 \uc804 1\/10\ub85c \ud76c\uc11d\ud558\uc5ec \ucd5c\uc885\ub18d\ub3c4 100 \u03bcg\/mL\ub85c \uc0ac\uc6a9\ud588\ub2e4.<\/p>\n

-HPLC \ubd84\uc11d\uc744 \uc704\ud55c Sample\uc758 \uc900\ube44(drug in rat plasma)
\nEP tube\uc5d0 200p, 20p pipette\uc744 \uc774\uc6a9\ud558\uc5ec Rat plasma 90 \u03bcL + \uacc4\uc5f4 \ud76c\uc11d\ud55c indomethacin 10\u03bcL + docetaxel (IS, 100\u03bcg\/mL) 10\u03bcL + ACN 90 \u03bcL\ub97c \uc21c\uc11c\ub300\ub85c \ub123\uc5b4\uc92c\ub2e4. (\ub123\ub294 \uc21c\uc11c\ub294 \ud608\uc7a5\u2192\uc57d\ubb3c\u2192ACN\uc21c).\ub530\ub77c\uc11c \ud608\uc7a5\uc5d0 \ub4e4\uc5b4\uac04 indomethacin\uc758 \ucd5c\uc885\ub18d\ub3c4\ub294 \uacc4\uc5f4 \ud76c\uc11d\ud55c \ub18d\ub3c4\uc758 1\/10\uc778 0.2, 0.5, 1 ,2, 5, 10\u03bcg\/mL<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc81c\ubaa9 & \uc2e4\ud5d8 \ubaa9\uc801 2. \ubc29\ubc95 3. \uacb0\uacfc 4. \uace0\ucc30 \ubcf8\ubb38\ub0b4\uc6a9 1. \uc81c\ubaa9 & \uc2e4\ud5d8 \ubaa9\uc801 \uc5ed\uc0c1 HPLC\/UV\ub97c \uc774\uc6a9\ud55c indomethacin \ubd84\uc11d. \ud2b9\uc774\uc131\uacfc \uc9c1\uc120\uc131\uc744 \ubd84\uc11d\ud558\uace0 \ubbf8\uc9c0\ub18d\ub3c4 \uad6c\ud558\uae30 \uc57d\ubb3c peak\uc640 retention time\uc744 \ud1b5\ud574 indomethacin\uacfc docetaxel \uc758 \ud2b9\uc774\uc131\uc744 \ud655\uc778\ud558\uace0, \uc0d8\ud50c\uc744 \uacc4\uc5f4 \ud76c\uc11d\uc2dc\ucf1c \uc9c1\uc120\uc131\uc744 \uad6c\ud558\uace0 \ubbf8\uc9c0 \uc57d\ubb3c\uc758 \ub18d\ub3c4\ub97c \uad6c\ud558\ub294 \uac83\uc774 \uc2e4\ud5d8\uc758 \ubaa9\uc801\uc774\ub2e4. 2. \ubc29\ubc95 – Standard samples\uc758 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[28589,48794,12123,48795,48793],"class_list":["post-38967","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-28589","tag-48794","tag-12123","tag-48795","tag-48793"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/38967","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=38967"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/38967\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=38967"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=38967"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=38967"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}