{"id":3915,"date":"2025-05-14T09:01:37","date_gmt":"2025-05-14T00:01:37","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/14\/3915\/"},"modified":"2025-05-14T09:01:37","modified_gmt":"2025-05-14T00:01:37","slug":"a-%ec%84%9c%ea%b0%95%eb%8c%80%ed%95%99%ea%b5%90-%ec%9d%bc%eb%b0%98%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%981-%ec%8b%a4%ed%97%98-11-plasmid-dna%ec%9d%98-%ec%a0%9c%ed%95%9c%ed%9a%a8%ec%86%8c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/14\/3915\/","title":{"rendered":"[A+] \uc11c\uac15\ub300\ud559\uad50 \uc77c\ubc18\uc0dd\ubb3c\ud559\uc2e4\ud5d81 \uc2e4\ud5d8 11. Plasmid DNA\uc758 \uc81c\ud55c\ud6a8\uc18c \uc808\ub2e8 \ubc0f \uc804\uae30\uc601\ub3d9 \ud480\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\tAbstract
\n2.\tIntroduction
\n3.\tMaterials & Methods
\n4.\tResult
\n5.\tDiscussion<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

Abstract
\nDNA \uc6b4\ubc18\uccb4\uc778 vector\ub294 \uc885\ub958\uac00 \ub2e4\uc591\ud558\uba70, DNA\uc758 \uc720\uc9c0\uc640 \ubcf5\uc81c\uac00 \uac00\ub2a5\ud55c DNA\ubd84\uc790\uc774\ub2e4. \uadf8 \uc911 plasmid\ub294 \uac00\uc7a5 \ub110\ub9ac \uc4f0\uc774\ub294 vector\uc774\uba70, circular\uad6c\uc870\uc758 \uc774\uc911\uac00\ub2e5 \ud615\ud0dc\ub97c \uac16\ub294\ub2e4. \uc774\ubc88 \uc2e4\ud5d8\uc5d0\uc11c\ub294 \uc81c\ud55c\ud6a8\uc18c(BamH I)\ub97c \uc774\uc6a9\ud558\uc5ec plasmid DNA(pUC4K)\ub97c \ubc18\uc751\uc2dc\ucf1c BamH I \uc778\uc2dd\ubd80\uc704\uc778 405bp\uc640 1669bp\uc5d0\uc11c plasmid DNA\ub97c \uc808\ub2e8\ud55c \ub2e4\uc74c \uadf8 \uacb0\uacfc\ubb3c\uc744 1% agarose gel\uc744 \uc774\uc6a9\ud558\uc5ec 100V\uc5d0\uc11c 30\ubd84\uac04 electrophoresis\ub97c \uc2e4\uc2dc\ud558\uace0 ladder\ub97c \uae30\uc900\uc73c\ub85c \uc798\ub9b0 band\uc640 \uc798\ub9ac\uc9c0 \uc54a\uc740 band\uc758 DNA \ud06c\uae30\uc5d0 \ub530\ub77c \uc5b4\ub5a4 \ucc28\uc774\uac00 \uc788\ub294\uc9c0 \ube44\uad50, \ubd84\uc11d\ud55c\ub2e4. \uc798\ub9ac\uc9c0 \uc54a\uc740 plasmid DNA(pUC4K)\uc758 \ucd1d \uae38\uc774\ub294 3966bp\uc774\uba70 405bp\uc640 1669bp\uc5d0\uc11c \uc798\ub9b0 Plasmid DNA\ub294 \uae38\uc774\uac00 1264bp, 2702bp\uc758 \ub450 DNA\ub85c \ub098\ub258\uba70, 1264bp\uae38\uc774\uc758 band\uac00 \ud06c\uae30\uac00 \uc81c\uc77c \uc791\uae30 \ub54c\ubb38\uc5d0 \uc804\uae30\uc601\ub3d9 \uacb0\uacfc \uc774\ub3d9\uc18d\ub3c4\uac00 \uc81c\uc77c \ube60\ub984\uc744 \uc54c \uc218 \uc788\uc5c8\ub2e4. \ub610\ud55c, \uc804\uae30\uc601\ub3d9 \uc2dc Supercoiled\ud615\ud0dc\ub294 linear\ud615\ud0dc\ubcf4\ub2e4 \uc18d\ub3c4\uac00 \ube68\ub77c \uc798\ub9ac\uc9c0 \uc54a\uc740 plasmid DNA\ub294 \ud06c\uae30\uac00 \uc81c\uc77c \ud06c\ub098 2702bp \ud06c\uae30\uc758 DNA\uc640 \ube44\uc2b7\ud55c \uc774\ub3d9\uc18d\ub3c4\ub97c \uac00\uc9d0\uc744 \uc54c \uc218 \uc788\uc5c8\ub2e4. \ubcf8 \uc2e4\ud5d8\uc5d0\uc11c \uc774\uc6a9\ud55c \uc81c\ud55c\ud6a8\uc18c(BamH I) \uc774\uc678\uc5d0 \ub2e4\ub978 \uc81c\ud55c\ud6a8\uc18c\uc778 EcoR I\ub97c plasmid DNA\uc640 \ubc18\uc751\uc2dc\ud0a4\uace0 \uadf8\uc5d0 \ub530\ub978 \uacb0\uacfc\ub97c \uc608\uc0c1\ud558\uc5ec \ube44\uad50\ud574\ubcf4\uc558\uc73c\uba70, agarose gel\uc758 \ub18d\ub3c4\ub97c \ub354 \ub192\uc600\uc744 \ub54c, \uc2e4\ud5d8 \uacfc\uc815 \ubc0f \uacb0\uacfc\uc758 \ubcc0\ud654\uc5d0 \ub300\ud574\uc11c\ub3c4 \uc608\uce21\ud574\ubcf4\uc558\ub2e4.
\nIntroduction
\n Vector\ub780, DNA \uc6b4\ubc18\uccb4\ub85c \uc720\uc804\ubb3c\uc9c8\uc758 \ucc98\ub9ac\uc640 \uc870\uc791 \uadf8\ub9ac\uace0 \uc6b4\ubc18\uc5d0 \ud544\uc694\ud558\ub2e4. Vector\ub294 \uc219\uc8fc \uc548\uc5d0\uc11c \uc548\uc815\uc801\uc778 \uc720\uc9c0\uc640 \ubcf5\uc81c\uac00 \uac00\ub2a5\ud558\uace0, \uc678\ubd80 DNA\ub97c \ud2b9\uc815\ubd80\uc704\uc5d0 \uc9d1\uc5b4\ub123\uac70\ub098 \uc81c\uac70\ud560 \uc218 \uc788\ub2e4. \ud2b9\uc815\ubd80\uc704\uc5d0 \uc0bd\uc785\ub41c \ud45c\uc801 \uc720\uc804\uc790\ub97c \uc99d\ud3ed\ud558\uae30 \uc704\ud558\uc5ec vector\ub294 DNA \ubcf5\uc81c\uac00 \uac00\ub2a5\ud558\ub3c4\ub85d origin of replication\uc744 \uac16\ub294\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Abstract 2. Introduction 3. Materials & Methods 4. Result 5. Discussion \ubcf8\ubb38\ub0b4\uc6a9 Abstract DNA \uc6b4\ubc18\uccb4\uc778 vector\ub294 \uc885\ub958\uac00 \ub2e4\uc591\ud558\uba70, DNA\uc758 \uc720\uc9c0\uc640 \ubcf5\uc81c\uac00 \uac00\ub2a5\ud55c DNA\ubd84\uc790\uc774\ub2e4. \uadf8 \uc911 plasmid\ub294 \uac00\uc7a5 \ub110\ub9ac \uc4f0\uc774\ub294 vector\uc774\uba70, circular\uad6c\uc870\uc758 \uc774\uc911\uac00\ub2e5 \ud615\ud0dc\ub97c \uac16\ub294\ub2e4. \uc774\ubc88 \uc2e4\ud5d8\uc5d0\uc11c\ub294 \uc81c\ud55c\ud6a8\uc18c(BamH I)\ub97c \uc774\uc6a9\ud558\uc5ec plasmid DNA(pUC4K)\ub97c \ubc18\uc751\uc2dc\ucf1c BamH I \uc778\uc2dd\ubd80\uc704\uc778 405bp\uc640 1669bp\uc5d0\uc11c plasmid DNA\ub97c \uc808\ub2e8\ud55c \ub2e4\uc74c \uadf8 \uacb0\uacfc\ubb3c\uc744 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[5955,7237,7236,7239],"class_list":["post-3915","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-5955","tag-7237","tag-7236","tag-7239"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3915","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3915"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3915\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3915"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3915"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3915"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}