{"id":39211,"date":"2026-05-02T09:02:44","date_gmt":"2026-05-02T00:02:44","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/05\/02\/39211\/"},"modified":"2026-05-02T09:02:44","modified_gmt":"2026-05-02T00:02:44","slug":"protein-electro-transfer-and-western-blot-%ec%8b%a4%ed%97%98%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/05\/02\/39211\/","title":{"rendered":"Protein electro-transfer and Western blot \uc2e4\ud5d8\ubcf4\uace0\uc11c"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Introduction
\n2. Method
\n3. Result
\n4. Discussion<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

I. Introduction
\nWestern blot \uc740 \ucc3e\uace0\uc790 \ud558\ub294 protein \uc758 antigen epitope \uc640 \ubc18\uc751\ud558\ub294 antibody \ub97c \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8 \ud63c\ud569\ubb3c \uc911\uc5d0\uc11c \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8(antigen)\uc744 \ucc3e\uc544\ub0b4\ub294 \ubc29\ubc95\uc774\ub2e4. \uc804\uae30 \uc601\ub3d9\uc744 \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 gel \uc0c1\uc5d0\uc11c \ud06c\uae30\uc5d0 \ub530\ub77c \ubd84\ub9ac\ud55c \ud6c4, membrane \uc5d0 transfer \ud55c\ub2e4. Membrane \uc5d0 \ubc1c\uad11\ud558\ub294 probe \uac00 \ubd99\uc5b4 \uc788\ub294 antibody \ub97c \ubd99\uc5ec\uc8fc\ub294 \ud56d\uc6d0-\ud56d\uccb4 \ubc18\uc751\uc744 \uc774\uc6a9\ud558\uc5ec \ucc3e\uace0\uc790 \ud558\ub294 \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc744 \uac80\ucd9c\ud55c\ub2e4. \uc5ec\ub7ec \uac00\uc9c0 \ub2e8\ubc31\uc9c8 \uc911\uc5d0\uc11c \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8\ub9cc\uc744 \ubcf4\ub294 \ubc29\ubc95\uc73c\ub85c\ub294 colorimetric, fluorescence, luminescence \ub4f1\uc774 \uc788\ub2e4.<\/p>\n

II. Material
\n8x Acrylamide gel, 5x Sample Buffer, 1x SDS running Buffer, 1x Transfer Buffer, Nitro cellulous membrane, 3M Paper, Transfer cassette, Gel Releaser, Protein marker, Blocking solution (5% skim milk), AP (Alkaline Phosphatase) Primary Antibody, HRP-conjugated Secondary Antibody, ECL Solution, 0.05% TBST, Tray, Vortex Mixer, tube, pipette, Disposable glove, Gel cassette stand, tank, DW, ep-tube, centrifuge, rocker<\/p>\n

III. Method
\n1. \ub2e8\ubc31\uc9c8 sample \uc744 \uc900\ube44\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Introduction 2. Method 3. Result 4. Discussion \ubcf8\ubb38\ub0b4\uc6a9 I. Introduction Western blot \uc740 \ucc3e\uace0\uc790 \ud558\ub294 protein \uc758 antigen epitope \uc640 \ubc18\uc751\ud558\ub294 antibody \ub97c \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8 \ud63c\ud569\ubb3c \uc911\uc5d0\uc11c \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8(antigen)\uc744 \ucc3e\uc544\ub0b4\ub294 \ubc29\ubc95\uc774\ub2e4. \uc804\uae30 \uc601\ub3d9\uc744 \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 gel \uc0c1\uc5d0\uc11c \ud06c\uae30\uc5d0 \ub530\ub77c \ubd84\ub9ac\ud55c \ud6c4, membrane \uc5d0 transfer \ud55c\ub2e4. Membrane \uc5d0 \ubc1c\uad11\ud558\ub294 probe \uac00 \ubd99\uc5b4 \uc788\ub294 antibody […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[34014,9619,425,40874,7373,33288,23352,49085,1293],"class_list":["post-39211","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-34014","tag-10-","tag-12-","tag-kwbooks","tag-protein-extraction","tag-western-blot","tag-23352","tag-49085","tag-1293"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/39211","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=39211"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/39211\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=39211"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=39211"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=39211"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}