{"id":3953,"date":"2025-05-15T09:00:35","date_gmt":"2025-05-15T00:00:35","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3953\/"},"modified":"2025-05-15T09:00:35","modified_gmt":"2025-05-15T00:00:35","slug":"changing-cell-culture-media-refreshing-the-media-a%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3953\/","title":{"rendered":"Changing cell culture media (Refreshing the media) A+\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title
\n2. Date
\n3. Name
\n4. Purpose
\n5. Materials
\n6. Methods
\n7. Results
\n8. Discussions
\n9. Project
\n10. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title
\nChanging cell culture media (Refreshing the media)<\/p>\n

2. Date<\/p>\n

3. Name<\/p>\n

4. Purpose
\n\uacc4\uc18d\ud574\uc11c \ubc30\uc591\uc774 \ud544\uc694\ud55c \uc138\ud3ec\ub294 \uc801\uc808\ud55c \uc601\uc591\ubd84\uc774 \uc9c0\uc18d\uc801\uc73c\ub85c \uacf5\uae09\ub418\uc5b4\uc57c \ud558\uba70, \uc801\uc808\ud55c pH\ub97c \uc720\uc9c0\uc2dc\ucf1c \uc8fc\uc5b4\uc57c \ud568\uc744 \uc774\ud574\ud55c\ub2e4. \ub610\ud55c \ubc30\uc9c0\ub97c \uad50\uccb4\ud558\ub294 \ubc29\ubc95\uc744 \uc775\ud78c\ub2e4.<\/p>\n

5. Materials
\nTamoxifen resistant MCF-7 cell (cultured in T25 flask), DPBS, Media (RPMI1640, FBS, P\/S), alcohol lamp, micropipette, tip, rack, beaker, 70% ethanol, tissue, clean bench<\/p>\n

6. Methods
\n\u2460 4\u2103\uc5d0\uc11c \ub0c9\uc7a5\ubcf4\uad00 \uc911\uc774\ub358 media\ub97c 37\u2103 water bath\uc5d0\uc11c \ucda9\ubd84\ud788 pre-warmed.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title Changing cell culture media (Refreshing the media) 2. Date 3. Name 4. Purpose \uacc4\uc18d\ud574\uc11c \ubc30\uc591\uc774 \ud544\uc694\ud55c \uc138\ud3ec\ub294 \uc801\uc808\ud55c \uc601\uc591\ubd84\uc774 \uc9c0\uc18d\uc801\uc73c\ub85c \uacf5\uae09\ub418\uc5b4\uc57c \ud558\uba70, \uc801\uc808\ud55c pH\ub97c \uc720\uc9c0\uc2dc\ucf1c \uc8fc\uc5b4\uc57c \ud568\uc744 \uc774\ud574\ud55c\ub2e4. \ub610\ud55c \ubc30\uc9c0\ub97c \uad50\uccb4\ud558\ub294 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7348,7347,7346],"class_list":["post-3953","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cell-culture","tag-media-change","tag-7346"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3953","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3953"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3953\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3953"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3953"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3953"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}