{"id":3955,"date":"2025-05-15T09:00:40","date_gmt":"2025-05-15T00:00:40","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3955\/"},"modified":"2025-05-15T09:00:40","modified_gmt":"2025-05-15T00:00:40","slug":"subculture-a%eb%a0%88%ed%8f%ac%ed%8a%b8%ec%9e%85%eb%8b%88%eb%8b%a4","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3955\/","title":{"rendered":"Subculture A+\ub808\ud3ec\ud2b8\uc785\ub2c8\ub2e4."},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title
\n2. Date
\n3. Name
\n4. Purpose
\n5. Materials
\n6. Methods
\n7. Results
\n8. Discussions
\n9. Project
\n10. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title
\nSubculture(\uacc4\ub300\ubc30\uc591)<\/p>\n

2. Date<\/p>\n

3. Name<\/p>\n

4. Purpose
\n\uc138\ud3ec\ub294 \ubd84\uc5f4\uc744 \uc704\ud574 \uc0c8\ub85c\uc6b4 \uacf5\uac04\uc744 \ud544\uc694\ub85c \ud558\uc9c0\ub9cc, \ubc30\uc591\uc811\uc2dc(flask)\uc758 \ud55c\uc815\ub41c \uacf5\uac04\uc5d0\uc11c\ub294 \uc138\ud3ec\uc99d\uc2dd\uc774 \uc81c\ud55c\ub420 \uc218 \uc788\ub2e4. \uacc4\ub300\ubc30\uc591(subculture)\uc744 \ud1b5\ud574 \uc138\ud3ec\uc99d\uc2dd\uc5d0 \ud544\uc694\ud55c \uc801\uc808\ud55c \uacf5\uac04\uc744 \uc81c\uacf5\ud574\uc57c \ud568\uc744 \uc774\ud574\ud55c\ub2e4. <\/p>\n

5. Materials
\nCells (cultured in T25 flask, 60\u339c dish), DPBS, Media (RPMI1640, FBS, P\/S), 0.05% Trypsin-EDTA solution, cell scraper, alcohol lamp, micropipette, tip, rack, beaker, T25 flask, 60\u339c dish, 1.5\u3396 ep-tube, 70% ethanol, tissue, incubator, centrifuge, clean bench<\/p>\n

6. Methods
\n\u2460 4\u2103\uc5d0\uc11c \ub0c9\uc7a5\ubcf4\uad00 \uc911\uc774\ub358 media\ub97c 37\u2103 water bath\uc5d0\uc11c \ucda9\ubd84\ud788 pre-warmed.
\n\u2461 Clean bench\uc5d0 \ub123\uae30 \uc804, pre-warmed media \ubc0f \ud544\uc694\ud55c \uc2e4\ud5d8\uae30\uad6c\ub4e4\uc744 70% \uc5d0\ud0c4\uc62c\ub85c \uc18c\ub3c5\ud55c\ub2e4. (Cell\uc774 \ub4e4\uc5b4\uc788\ub294 flask, dish\ub294 \uc5d0\ud0c4\uc62c\ub85c \uc18c\ub3c5\ud558\uc9c0 \uc54a\uace0 clean bench\ub85c \uc62e\uaca8\uc900\ub2e4.)
\n\u2462 \uc138\ud3ec\uc8fc\ub97c \ubc30\uc591 \uc911\uc778 \ubc30\uc591\uc811\uc2dc \ub0b4\ubd80\uc758 media\ub97c micropipette\uc73c\ub85c aspiration & discard.
\n* Tube\uc5d0 \uc18c\ubd84\ub41c DPBS, media\ub294 \uc0ac\uc6a9 \uc804\ud6c4\ub85c \ub69c\uaed1 \uc8fc\ubcc0, \ub69c\uaed1 \ub0b4\ubd80,tube \uc785\uad6c\ub97c \ud654\uc5fc\uba78\uade0\ud558\uace0 \uc0ac\uc6a9\ud558\uba70, ep-tube\uc5d0 \uc18c\ubd84\ub41c Trypsin-EDTA\uc758 \uacbd\uc6b0 \ud654\uc5fc\uba78\uade0\uc744 \uc0dd\ub7b5\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title Subculture(\uacc4\ub300\ubc30\uc591) 2. Date 3. Name 4. Purpose \uc138\ud3ec\ub294 \ubd84\uc5f4\uc744 \uc704\ud574 \uc0c8\ub85c\uc6b4 \uacf5\uac04\uc744 \ud544\uc694\ub85c \ud558\uc9c0\ub9cc, \ubc30\uc591\uc811\uc2dc(flask)\uc758 \ud55c\uc815\ub41c \uacf5\uac04\uc5d0\uc11c\ub294 \uc138\ud3ec\uc99d\uc2dd\uc774 \uc81c\ud55c\ub420 \uc218 \uc788\ub2e4. \uacc4\ub300\ubc30\uc591(subculture)\uc744 \ud1b5\ud574 \uc138\ud3ec\uc99d\uc2dd\uc5d0 \ud544\uc694\ud55c \uc801\uc808\ud55c \uacf5\uac04\uc744 \uc81c\uacf5\ud574\uc57c \ud568\uc744 \uc774\ud574\ud55c\ub2e4. 5. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7353,7352,7351],"class_list":["post-3955","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cell-maintaining","tag-subculture","tag-7351"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3955","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3955"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3955\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3955"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3955"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3955"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}