{"id":3956,"date":"2025-05-15T09:00:43","date_gmt":"2025-05-15T00:00:43","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3956\/"},"modified":"2025-05-15T09:00:43","modified_gmt":"2025-05-15T00:00:43","slug":"cell-counting-and-seeding-on-plate-for-downstream-experiment-a%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3956\/","title":{"rendered":"Cell counting and seeding on plate for downstream experiment A+\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title
\n2. Date
\n3. Name
\n4. Purpose
\n5. Materials
\n6. Methods
\n7. Results
\n8. Discussions
\n9. Project
\n10. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title
\nCell counting and seeding on plate for downstream experiment<\/p>\n

2. Date<\/p>\n

3. Name<\/p>\n

4. Purpose
\nHemocytometer\uc758 \uc6d0\ub9ac\ub97c \uc774\ud574\ud558\uace0, \uc774\ub97c \ud1b5\ud574 \uc2e4\ud5d8\uc5d0 \ud544\uc694\ub85c \ud558\ub294 \uc801\uc815\ub7c9\uc758 \uc138\ud3ec \uc218\ub97c \uacc4\uc0b0\ud560 \uc218 \uc788\ub2e4. <\/p>\n

5. Materials
\nMCF-7 Cells (cultured in T25 flask), DPBS, 0.05% Trypsin-EDTA solution, Media (RPMI1640, FBS, P\/S), alcohol lamp, pipette aid, serological pipette, incubator, conical tube, centrifuge, micropipette, tip, rack, beaker, 1.5\u3396 ep-tube, trypan blue solution, hemocytometer, 35\u339c dish, 70% ethanol, tissue, clean bench, \uad11\ud559\ud604\ubbf8\uacbd, \uacc4\uc218\uae30<\/p>\n

6. Methods
\n\u2460 4\u2103\uc5d0\uc11c \ub0c9\uc7a5\ubcf4\uad00 \uc911\uc774\ub358 media\ub97c 37\u2103 water bath\uc5d0\uc11c \ucda9\ubd84\ud788 pre-warmed.
\n\u2461 Clean bench\uc5d0 \ub123\uae30 \uc804, pre-warmed media \ubc0f \ud544\uc694\ud55c \uc2e4\ud5d8\uae30\uad6c\ub4e4\uc744 70% \uc5d0\ud0c4\uc62c\ub85c \uc18c\ub3c5\ud55c\ub2e4. (Cell\uc774 \ub4e4\uc5b4\uc788\ub294 flask\ub294 \uc5d0\ud0c4\uc62c\ub85c \uc18c\ub3c5\ud558\uc9c0 \uc54a\uace0 clean bench\ub85c \uc62e\uaca8\uc900\ub2e4.)
\n\u2462 \uc138\ud3ec\uc8fc\ub97c \ubc30\uc591 \uc911\uc778 flask \ub0b4\ubd80\uc758 media\ub97c pipette aid\ub97c \uc774\uc6a9\ud574 aspiration & discard.
\n\u2463 DPBS washing: DPBS 3\u3396\uc744 flask\uc5d0 \uc870\uc2ec\ud788 \ubd84\uc8fc\ud558\uace0 \uc88c\uc6b0\ub85c gently washing \ud574\uc900 \ud6c4, DPBS\ub97c \ub2e4\uc2dc aspiration & discard.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title Cell counting and seeding on plate for downstream experiment 2. Date 3. Name 4. Purpose Hemocytometer\uc758 \uc6d0\ub9ac\ub97c \uc774\ud574\ud558\uace0, \uc774\ub97c \ud1b5\ud574 \uc2e4\ud5d8\uc5d0 \ud544\uc694\ub85c \ud558\ub294 \uc801\uc815\ub7c9\uc758 \uc138\ud3ec \uc218\ub97c \uacc4\uc0b0\ud560 \uc218 \uc788\ub2e4. 5. Materials […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7354,7355,3256],"class_list":["post-3956","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cell-counting","tag-cell-seeding","tag-hemocytometer"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3956","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3956"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3956\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3956"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3956"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3956"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}