{"id":3972,"date":"2025-05-15T09:00:57","date_gmt":"2025-05-15T00:00:57","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3972\/"},"modified":"2025-05-15T09:00:57","modified_gmt":"2025-05-15T00:00:57","slug":"colony-formation-assay-a%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3972\/","title":{"rendered":"Colony formation assay A+\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title
\n2. Date
\n3. Name
\n4. Purpose
\n5. Materials
\n6. Methods
\n7. Results
\n8. Discussions
\n9. Project
\n10. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title
\nColony formation assay<\/p>\n

2. Date<\/p>\n

3. Name<\/p>\n

4. Purpose
\nIn vitro\uc5d0\uc11c \uc138\ud3ec \uad70\uc9d1 \ud615\uc131 \uc815\ub3c4\ub97c \ubd84\uc11d\ud558\ub294 \uac83\uc744 \ud1b5\ud574 \uc138\ud3ec \ud65c\uc131\uc744 \ud655\uc778\ud560 \uc218 \uc788\ub2e4.<\/p>\n

5. Materials
\nMCF-7 Cells (cultured in 35\u339c dish), DPBS, fixation solution (Methanol : Acetic acid = 7:1), 0.2% crystal violet solution, D.W, micropipette, tip, beaker, 70% ethanol, tissue<\/p>\n

6. Methods
\n\u2460 \uc2e4\ud5d8\ud14c\uc774\ube14 70% \uc5d0\ud0c4\uc62c \uc18c\ub3c5 \ub4f1 \uc900\ube44 \ud6c4, 37\u2103 CO2 incubator\uc5d0\uc11c dish\ub97c \uaebc\ub0b4\uc628\ub2e4.
\n\u2461 Pipette\uc744 \uc774\uc6a9\ud574 RPMI media\ub97c \uc81c\uac70\ud558\uace0, DPBS\ub85c 1\u3396\uc529 \ub450 \ubc88 rinse (wash)\ud574\uc900\ub2e4.
\n\u2462 \uc900\ube44\ub41c fixation solution 200\u3395\uc744 \ubd84\uc8fc\ud55c \ud6c4, \uace8\uace0\ub8e8 \ud37c\uc9c8 \uc218 \uc788\uac8c \ubd80\ub4dc\ub7fd\uac8c \ud754\ub4e0\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title Colony formation assay 2. Date 3. Name 4. Purpose In vitro\uc5d0\uc11c \uc138\ud3ec \uad70\uc9d1 \ud615\uc131 \uc815\ub3c4\ub97c \ubd84\uc11d\ud558\ub294 \uac83\uc744 \ud1b5\ud574 \uc138\ud3ec \ud65c\uc131\uc744 \ud655\uc778\ud560 \uc218 \uc788\ub2e4. 5. Materials MCF-7 Cells (cultured in 35\u339c dish), […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7362,7361,7363],"class_list":["post-3972","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-colony-formation","tag-7361","tag-7363"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3972","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3972"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3972\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3972"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3972"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3972"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}