{"id":3985,"date":"2025-05-15T09:01:08","date_gmt":"2025-05-15T00:01:08","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3985\/"},"modified":"2025-05-15T09:01:08","modified_gmt":"2025-05-15T00:01:08","slug":"cryopreservation-of-mammalian-cells-a%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3985\/","title":{"rendered":"Cryopreservation of mammalian cells A+\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\tTitle<\/p>\n

2.\tPurpose<\/p>\n

3.\tMaterials<\/p>\n

4.\tMethods<\/p>\n

5.\tResults<\/p>\n

6.\tDiscussions<\/p>\n

7.\tProject
\n(1) \ub610 \ub2e4\ub978 cryoprotective agent (cryoprotectant)\uc5d0\ub294 \uc5b4\ub5a4 \uac83\ub4e4\uc774 \uc788\uc744\uae4c?<\/p>\n

8.\tReference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

4. Purpose
\n\uc138\ud3ec\ub97c \uc7a5\uae30\uac04 \uacc4\ub300\ubc30\uc591\uc744 \ud558\uac8c \ub418\uba74 \uc624\uc5fc\uc73c\ub85c \uc778\ud574 \uc138\ud3ec\uac00 \uc18c\uc2e4\ub418\uac70\ub098 \ub3cc\uc5f0\ubcc0\uc774\uac00 \uc0dd\uaca8\ub0a0 \uc218 \uc788\ub2e4. \ub530\ub77c\uc11c \uc774\ub97c \ub300\ube44\ud558\uae30 \uc704\ud574 \ubbf8\ub9ac \ucda9\ubd84\ud55c \uc591\uc758 \uc138\ud3ec\ub97c \ud655\ubcf4\ud558\uace0, \uc7a5\uae30\uac04 \uc548\uc815\ud558\uac8c \uc720\uc9c0\u00b7\ubcf4\uad00\ud560 \uc218 \uc788\ub3c4\ub85d cell stock\uc744 \ub9cc\ub4e4\uc5b4 \ub3d9\uacb0 \ubcf4\uc874\uc2dc\ud0a8\ub2e4.<\/p>\n

5. Materials
\nMCF-7 Cells (cultured in T25 flask), DPBS, 0.05% Trypsin-EDTA solution, Media (RPMI1640, FBS, P\/S), DMSO, alcohol lamp, micropipette, tip, rack, beaker, 1.5\u3396 ep-tube, cryogenic vial, 70% ethanol, tissue, incubator, centrifuge, clean bench, (2-propanol, cryo freezing container, liquid nitrogen container) <\/p>\n

6. Methods
\n\u2460 4\u2103\uc5d0\uc11c \ub0c9\uc7a5\ubcf4\uad00 \uc911\uc774\ub358 media\ub97c 37\u2103 water bath\uc5d0\uc11c \ucda9\ubd84\ud788 pre-warmed.
\n\u2461 Clean bench\uc5d0 \ub123\uae30 \uc804, pre-warmed media \ubc0f \ud544\uc694\ud55c \uc2e4\ud5d8\uae30\uad6c\ub4e4\uc744 70% \uc5d0\ud0c4\uc62c\ub85c \uc18c\ub3c5\ud55c\ub2e4. (Cell\uc774 \ub4e4\uc5b4\uc788\ub294 flask\ub294 \uc5d0\ud0c4\uc62c\ub85c \uc18c\ub3c5\ud558\uc9c0 \uc54a\uace0 clean bench\ub85c \uc62e\uaca8\uc900\ub2e4.)<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Purpose 3. Materials 4. Methods 5. Results 6. Discussions 7. Project (1) \ub610 \ub2e4\ub978 cryoprotective agent (cryoprotectant)\uc5d0\ub294 \uc5b4\ub5a4 \uac83\ub4e4\uc774 \uc788\uc744\uae4c? 8. Reference \ubcf8\ubb38\ub0b4\uc6a9 4. Purpose \uc138\ud3ec\ub97c \uc7a5\uae30\uac04 \uacc4\ub300\ubc30\uc591\uc744 \ud558\uac8c \ub418\uba74 \uc624\uc5fc\uc73c\ub85c \uc778\ud574 \uc138\ud3ec\uac00 \uc18c\uc2e4\ub418\uac70\ub098 \ub3cc\uc5f0\ubcc0\uc774\uac00 \uc0dd\uaca8\ub0a0 \uc218 \uc788\ub2e4. \ub530\ub77c\uc11c \uc774\ub97c \ub300\ube44\ud558\uae30 \uc704\ud574 \ubbf8\ub9ac \ucda9\ubd84\ud55c \uc591\uc758 \uc138\ud3ec\ub97c \ud655\ubcf4\ud558\uace0, \uc7a5\uae30\uac04 \uc548\uc815\ud558\uac8c \uc720\uc9c0\u00b7\ubcf4\uad00\ud560 \uc218 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7370,7351,7343,7371],"class_list":["post-3985","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cell-stock","tag-7351","tag-7343","tag-7371"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3985","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3985"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3985\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3985"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3985"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3985"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}