{"id":3986,"date":"2025-05-15T09:01:09","date_gmt":"2025-05-15T00:01:09","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3986\/"},"modified":"2025-05-15T09:01:09","modified_gmt":"2025-05-15T00:01:09","slug":"adherent-cell-lysis-for-protein-extraction-a%eb%a0%88%ed%8f%ac%ed%8a%b8%ec%9e%85%eb%8b%88%eb%8b%a4","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3986\/","title":{"rendered":"Adherent Cell lysis for Protein extraction A+\ub808\ud3ec\ud2b8\uc785\ub2c8\ub2e4."},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title
\n2. Date
\n3. Name
\n4. Purpose
\n5. Materials
\n6. Methods
\n7. Results
\n8. Discussions
\n9. Project
\n10. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title
\nAdherent Cell lysis for Protein extraction<\/p>\n

2. Date<\/p>\n

3. Name<\/p>\n

4. Purpose
\nCell lysis (or cellular disruption)\uc5d0 \uc0ac\uc6a9\ub418\ub294 \uc5ec\ub7ec \uac00\uc9c0 \ubb3c\ub9ac\uc801, \ud654\ud559\uc801 \ubc29\ubc95\uc744 \uc54c\uc544\ubcf8 \ud6c4, RIPA lysis buffer\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc138\ud3ec\ub97c \ud30c\uad34\ud558\uace0 cellular protein\uc744 \uc5bb\uc744 \uc218 \uc788\ub2e4.<\/p>\n

5. Materials
\nMCF-7 Cells (cultured in 60\u339c dish), Ice cold DPBS, Ice cold RIPA lysis buffer, micropipette, tip, rack, beaker, cell scraper, ep-tube, centrifuge, ice, tissue, 70% ethanol<\/p>\n

6. Methods
\n\u2460 Micropipette\uc73c\ub85c media\ub97c \uc81c\uac70\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title Adherent Cell lysis for Protein extraction 2. Date 3. Name 4. Purpose Cell lysis (or cellular disruption)\uc5d0 \uc0ac\uc6a9\ub418\ub294 \uc5ec\ub7ec \uac00\uc9c0 \ubb3c\ub9ac\uc801, \ud654\ud559\uc801 \ubc29\ubc95\uc744 \uc54c\uc544\ubcf8 \ud6c4, RIPA lysis buffer\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc138\ud3ec\ub97c \ud30c\uad34\ud558\uace0 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7372,7373,7374],"class_list":["post-3986","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cell-lysis","tag-protein-extraction","tag-7374"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3986","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3986"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3986\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3986"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3986"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3986"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}