{"id":3987,"date":"2025-05-15T09:01:10","date_gmt":"2025-05-15T00:01:10","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3987\/"},"modified":"2025-05-15T09:01:10","modified_gmt":"2025-05-15T00:01:10","slug":"bca-assay-a%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/05\/15\/3987\/","title":{"rendered":"BCA assay A+\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Title
\n2. Date
\n3. Name
\n4. Purpose
\n5. Materials
\n6. Methods
\n7. Results
\n8. Discussions
\n9. Project
\n10. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. Title
\nProtein quantification : BCA assay<\/p>\n

2. Date<\/p>\n

3. Name<\/p>\n

4. Purpose
\n\ub2e8\ubc31\uc9c8 \uc815\ub7c9 \ubd84\uc11d \ubc29\ubc95 \uc911 colorimetric method\uc758 \uc77c\uc885\uc778 BCA assay\ub97c \uc218\ud589\ud558\uc5ec \ud45c\uc900 \ub2e8\ubc31\uc9c8\uc758 \ud761\uad11\ub3c4\ub97c \uc774\uc6a9\ud55c \ud45c\uc900\uace1\uc120\uc744 \uadf8\ub9b0 \ud6c4, \ubbf8\uc9c0\uc758 \ub2e8\ubc31\uc9c8 \uc2dc\ub8cc \ub18d\ub3c4\ub97c \uad6c\ud574\ubcf8\ub2e4.<\/p>\n

5. Materials
\nExtracted proteins (from MCF-7 cells), Bovine serum albumin (BSA) protein, nuclease free water, BCA reagent A & B, micropipette, tip, rack, 96well plate, 1.5\u3396 ep-tube, tissue, 70% EtOH, incubator, plate reader<\/p>\n

6. Methods
\n[1] Preparation of standard protein (BSA)
\n2\u338e\/\u3396 BSA stock (\uc870\ubcc4\ub85c 80\u3395\uc529 \uc81c\uacf5\ub428)\uc73c\ub85c serial dilution\uc744 \uc9c4\ud589\ud558\uc5ec 7\uac1c\uc758 ep-tube\uc5d0 \uac01\uac01 0, 0.025, 0.125, 0.25, 0.5, 1, 2\u338e\/\u3396 \ub18d\ub3c4\uc758 BSA\uc744 \ucd5c\uc18c 30\u3395\uc529 \uc900\ube44\ud55c\ub2e4.<\/p>\n

2\u338e\/\u3396
\n80\u3395(2\u338e\/\u3396 BSA)
\n30\u3395
\n1\u338e\/\u3396
\n50\u3395(2\u338e\/\u3396 BSA) + 50\u3395(D.W)
\n60\u3395
\n0.5\u338e\/\u3396
\n40\u3395(1\u338e\/\u3396 BSA) + 40\u3395(D.W)
\n40\u3395
\n0.25\u338e\/\u3396
\n40\u3395(0.5\u338e\/\u3396 BSA) + 40\u3395(D.W)
\n40\u3395
\n0.125\u338e\/\u3396
\n40\u3395(0.25\u338e\/\u3396 BSA) + 40\u3395(D.W)
\n60\u3395
\n0.025\u338e\/\u3396
\n20\u3395(0.125\u338e\/\u3396 BSA) + 80\u3395(D.W)
\n100\u3395
\n0\u338e\/\u3396
\n40\u3395(D.W)
\n40\u3395<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Title 2. Date 3. Name 4. Purpose 5. Materials 6. Methods 7. Results 8. Discussions 9. Project 10. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Title Protein quantification : BCA assay 2. Date 3. Name 4. Purpose \ub2e8\ubc31\uc9c8 \uc815\ub7c9 \ubd84\uc11d \ubc29\ubc95 \uc911 colorimetric method\uc758 \uc77c\uc885\uc778 BCA assay\ub97c \uc218\ud589\ud558\uc5ec \ud45c\uc900 \ub2e8\ubc31\uc9c8\uc758 \ud761\uad11\ub3c4\ub97c \uc774\uc6a9\ud55c \ud45c\uc900\uace1\uc120\uc744 \uadf8\ub9b0 \ud6c4, \ubbf8\uc9c0\uc758 \ub2e8\ubc31\uc9c8 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7376,7375,315],"class_list":["post-3987","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-bca-assay","tag-7375","tag-315"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3987","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=3987"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/3987\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=3987"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=3987"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=3987"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}