{"id":41217,"date":"2026-05-22T09:02:23","date_gmt":"2026-05-22T00:02:23","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/05\/22\/41217\/"},"modified":"2026-05-22T09:02:23","modified_gmt":"2026-05-22T00:02:23","slug":"%ec%83%9d%eb%aa%85%ea%b3%bc%ed%95%99%ec%8b%a4%ed%97%98-elisa-%ec%8b%a4%ed%97%98-%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/05\/22\/41217\/","title":{"rendered":"\uc0dd\uba85\uacfc\ud559\uc2e4\ud5d8 ELISA \uc2e4\ud5d8 \ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Introduction
\n\u25fc \uc2e4\ud5d8 \ubaa9\uc801
\n\ud56d\uc6d0\uc5d0 \ub300\ud55c \ud56d\uccb4\uc758 \ud2b9\uc774\uc801 \uacb0\ud569\uc744 \uc774\uc6a9\ud558\ub294 ELISA\ubc29\ubc95 \ubc0f \uc6d0\ub9ac\uc5d0 \ub300\ud574 \uc774\ud574\ud558\uace0,
\nantibody\ub18d\ub3c4\uc5d0 \ub530\ub978 \ud761\uad11\ub3c4\ub97c \ub098\ud0c0\ub0b4\ub294 4PL curve\ub97c \uc774\uc6a9\ud574 antibody\uac00 antigen
\n\uc5d0 \uc5bc\ub9c8\ub098 \uac15\ud558\uac8c \ubd99\uc5c8\ub294\uc9c0 \uc54c \uc218 \uc788\ub2e4.
\n\u25fc \uc774\ub860
\n\u2460 \ud56d\uc6d0-\ud56d\uccb4\ubc18\uc751
\n\u2461 ELISA
\n1) Direct ELISA (\uc9c1\uc811 ELISA)
\n2) Indirect ELISA
\n3) Sandwich ELISA
\n4) Competitive ELISA
\n\u2462 4PL Regression<\/p>\n

2. Methods<\/p>\n

3. Result<\/p>\n

4. Discussion<\/p>\n

5. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\u25fc \uc2e4\ud5d8 \ubaa9\uc801
\n\ud56d\uc6d0\uc5d0 \ub300\ud55c \ud56d\uccb4\uc758 \ud2b9\uc774\uc801 \uacb0\ud569\uc744 \uc774\uc6a9\ud558\ub294 ELISA\ubc29\ubc95 \ubc0f \uc6d0\ub9ac\uc5d0 \ub300\ud574 \uc774\ud574\ud558\uace0,
\nantibody\ub18d\ub3c4\uc5d0 \ub530\ub978 \ud761\uad11\ub3c4\ub97c \ub098\ud0c0\ub0b4\ub294 4PL curve\ub97c \uc774\uc6a9\ud574 antibody\uac00 antigen
\n\uc5d0 \uc5bc\ub9c8\ub098 \uac15\ud558\uac8c \ubd99\uc5c8\ub294\uc9c0 \uc54c \uc218 \uc788\ub2e4.
\n\u25fc \uc774\ub860
\n\u2460 \ud56d\uc6d0-\ud56d\uccb4\ubc18\uc751
\n\ud56d\uccb4(antibody) \ub610\ub294 \uba74\uc5ed\uae00\ub85c\ubd88\ub9b0(immunoglobulin,Ig)\uc740 \ud56d\uc6d0(antigen)\uacfc \ud2b9\uc774\uc801 \uacb0\ud569\uc744 \ud558\uc5ec \ud56d\uc6d0-\ud56d\uccb4 \ubc18\uc751\uc744 \uc77c\uc73c\ud0a4\ub294 \ubb3c\uc9c8\uc774\ub2e4. \uac00\uc7a5 \ub300\ud45c\uc801\uc778 \ud56d\uccb4\uc5d0\ub294 \uba74\uc5ed\uae00\ub85c\ubd88\ub9b0G(Immunoglobulin G)\uac00 \uc788\ub2e4. \uc774\ub7f0 \ud56d\uccb4\uc758 \uae30\ubcf8 \uad6c\uc870\ub294 Y\uc790 \ubaa8\uc591\uc774\uba70, heavy chain 2\uac1c\uc640 light chain 2\uac1c\uac00 \ube44\uacf5\uc720\uacb0\ud569\uacfc \uc774\ud669\uacb0\ud569\uc73c\ub85c \uc5f0\uacb0\ub41c 4\uac1c\uc758 \ud3f4\ub9ac\ud3a9\ud0c0\uc774\ub4dc\ub97c \uac00\uc9c0\uace0 \uc788\ub2e4. IgG\uc758 Y\uc790 \ubaa8\uc591\uc758 \uac00\uc9c0\ubd80\ubd84\uacfc \ubc1b\uce68\ubd80\ubd84\uc744 \ub098\ub204\ub294 \ubd80\ubd84\uc744 \uacbd\ucca9\uc774\ub77c\uace0 \ud558\ub294\ub370, \uc774 \uacbd\ucca9\uc601\uc5ed\uc744 \uae30\uc900\uc73c\ub85c \uac00\uc9c0\ubd80\ubd84\uc740 Fab, \ubc1b\uce68\ubd80\ubd84\uc740 Fc\uc601\uc5ed\uc73c\ub85c \ub098\ub208\ub2e4. \ud56d\uccb4\ub294 \uc138\uade0\uc774\ub098 \ubc14\uc774\ub7ec\uc2a4 \uac19\uc740 \uc678\ubd80 \ud56d\uc6d0\uacfc \ub2e8\ub2e8\ud558\uac8c \ud2b9\uc774\uc801\uc73c\ub85c \uacb0\ud569\ud55c\ub2e4
\n\ud56d\uc6d0-\ud56d\uccb4\ubc18\uc751\uc740 \uba74\uc5ed \uccb4\uacc4\uc5d0\uc11c \uc138\uade0\uc774\ub098 \ubc14\uc774\ub7ec\uc2a4 \uac19\uc740 \uc678\ubd80\ud56d\uc6d0\ub4e4\uacfc \ud2b9\uc774\uc801\uc73c\ub85c \uacb0\ud569\ud574 \ud56d\uc6d0\uc744 \uc778\uc2dd\ud558\uac8c\ud558\uace0 \ubb34\ub825\ud654\uc2dc\ud0a4\uae30 \uc704\ud55c \ud56d\uc6d0\uacfc \ud56d\uccb4 \uc0ac\uc774\uc758 \ud2b9\uc815\ud55c \ud654\ud559 \uc0c1\ud638\uc791\uc6a9\uc744 \ub9d0\ud55c\ub2e4. \ud56d\uc6d0-\ud56d\uccb4 \uacb0\ud569\uc758 \ud2b9\uc774\uc131\uc740 \uc804\ud558, \uadf9\uc131, \ube44\uadf9\uc131, \uc218\uc18c\uacb0\ud569 \ub4f1\uc774 \ub2e4\ub978 \uc794\uae30\ub4e4\uc758 \uc704\uce58\uc640 \ud615\ud0dc\uc5d0 \ub530\ub77c \ud56d\uc6d0\uacfc \ud56d\uc6d0\uacb0\ud569\uc790\ub9ac \uc0ac\uc774\uc758 \ud654\ud559\uc801 \uc0c1\ubcf4\uc131\uc744 \ubd80\uc5ec \ubc1b\uc544 \uc5bb\uac8c \ub41c\ub2e4. \ud56d\uc6d0\uacfc \ud56d\uccb4\uc758 \uacb0\ud569\uc740 \uc720\ub3c4\uc801\ud569(induced fit)\uc5d0 \uc758\ud574 \uac01\uac01\uc758 \uc0c1\ubcf4\uc131 \uc794\uae30\ub4e4\uc774 \ucda9\ubd84\ud788 \uc0c1\ud638\uc791\uc6a9\ud560 \uc218 \uc788\ub3c4\ub85d \uc77c\uc5b4\ub09c\ub2e4.<\/p>\n

\u2461 ELISA
\n– ELISA\ub780?
\nELISA(Enzyme-Linked ImmunoSorbent Assay)\ub294 antigen-antibody interaction\uc744 \uc774\uc6a9\ud574 antigen\uc774\ub098 antibody\ub97c \uc815\uc131, \uc815\ub7c9\ud654\ud560 \uc218 \uc788\ub294 \uc2e4\ud5d8\ubc95\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Introduction \u25fc \uc2e4\ud5d8 \ubaa9\uc801 \ud56d\uc6d0\uc5d0 \ub300\ud55c \ud56d\uccb4\uc758 \ud2b9\uc774\uc801 \uacb0\ud569\uc744 \uc774\uc6a9\ud558\ub294 ELISA\ubc29\ubc95 \ubc0f \uc6d0\ub9ac\uc5d0 \ub300\ud574 \uc774\ud574\ud558\uace0, antibody\ub18d\ub3c4\uc5d0 \ub530\ub978 \ud761\uad11\ub3c4\ub97c \ub098\ud0c0\ub0b4\ub294 4PL curve\ub97c \uc774\uc6a9\ud574 antibody\uac00 antigen \uc5d0 \uc5bc\ub9c8\ub098 \uac15\ud558\uac8c \ubd99\uc5c8\ub294\uc9c0 \uc54c \uc218 \uc788\ub2e4. \u25fc \uc774\ub860 \u2460 \ud56d\uc6d0-\ud56d\uccb4\ubc18\uc751 \u2461 ELISA 1) Direct ELISA (\uc9c1\uc811 ELISA) 2) Indirect ELISA 3) Sandwich ELISA 4) Competitive ELISA \u2462 4PL […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[46753,51718,51719,12093,51716,8836,1295,51717],"class_list":{"0":"post-41217","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-uncategorized","7":"tag-46753","8":"tag-4pl","9":"tag-4pl-regression","10":"tag-elisa","12":"tag-8836","13":"tag-1295","14":"tag-51717"},"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/41217","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=41217"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/41217\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=41217"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=41217"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=41217"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}