{"id":43029,"date":"2026-06-10T09:00:33","date_gmt":"2026-06-10T00:00:33","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43029\/"},"modified":"2026-06-10T09:00:33","modified_gmt":"2026-06-10T00:00:33","slug":"%ec%83%9d%eb%aa%85%ea%b3%bc%ed%95%99%ec%8b%a4%ed%97%981-2%ec%a3%bc%ec%b0%a8-%eb%b3%b4%ea%b3%a0%ec%84%9c-buffer-%ec%a0%9c%ec%a1%b0","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43029\/","title":{"rendered":"\uc0dd\uba85\uacfc\ud559\uc2e4\ud5d81 2\uc8fc\ucc28 \ubcf4\uace0\uc11c Buffer \uc81c\uc870"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumbnail\/2024\/06\/21\/data30033507-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumbnail\/2024\/06\/21\/data30033507-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>1. \uc2e4\ud5d8 \ubaa9\ud45c<br \/>\n2. Introduction<br \/>\n3. \uc2e4\ud5d8\ub3c4\uad6c<br \/>\n4. \uc2e4\ud5d8\ubc29\ubc95<br \/>\n5. \uacb0\uacfc: Buffer \uc81c\uc870\ud45c 1, Buffer \uc81c\uc870\ud45c 2<br \/>\n6. Discussion<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p>1. \uc2e4\ud5d8 \ubaa9\ud45c<br \/>\nColumn Chromatography\ub97c \ud558\uae30 \uc704\ud574 \ud544\uc694\ud55c Buffer\ub97c \uc81c\uc870\ud55c\ub2e4. Tris\uc640 HCl \ubc0f 3\ucc28 \uc99d\ub958\uc218\ub97c \uc774\uc6a9\ud558\uc5ec pH7.4\uc778 1M\uc758 Tris-HCl\uc744 \uc81c\uc870\ud558\ub294 \uc2e4\ud5d8\uc744 \uc9c4\ud589\ud558\uc600\ub2e4. 1M\uc758 Tris-HCl, MgSO4\uc640 NaCl\uc758 \ub18d\ub3c4\ub97c \ubc14\ud0d5\uc73c\ub85c \ub2e4\uc591\ud55c \ub18d\ub3c4\uc758 Elution buffer\uc758 \uad6c\uc131\uc131\ubd84\uc758 \uc6a9\ub7c9\uc744 \uacc4\uc0b0\ud558\uc600\ub2e4.<br \/>\n2. Introduction<br \/>\n(1) Buffer\ub780 \ubb34\uc5c7\uc778\uac00?<br \/>\n\ub2e4\uc591\ud55c \ub300\uc0ac \ud65c\ub3d9\uc774 \uc774\ub8e8\uc5b4\uc9c0\ub294 \uc0dd\uba85\uccb4 \ub0b4\uc758 pH\uac00 \uc77c\uc815\ud55c \ubc94\uc704\ub97c \ubc97\uc5b4\ub098\uba74, \uc0dd\uba85\uc744 \uc720\uc9c0\ud558\ub294 \ub370\uc5d0 \ud544\uc694\ud55c \ub300\uc0ac\uacfc\uc815\uc774 \uc77c\uc5b4\ub098\uc9c0 \ubabb\ud558\ub294 \uc0c1\ud669\uc774 \ubc1c\uc0dd\ud558\uac8c \ub41c\ub2e4. \ub530\ub77c\uc11c \uc0dd\uba85\uccb4 \ub0b4\uc5d0\uc11c \uc801\uc815\ud55c pH\ub97c \uc720\uc9c0\ud558\ub294 \uac83\uc774 \ub9e4\uc6b0 \uc911\uc694\ud558\ub2e4.<br \/>\n\ub9c8\ucc2c\uac00\uc9c0\ub85c \uc778\uac04\uc758 \ub300\uc0ac\uacfc\uc815\uc744 \uc5f0\uad6c\ud558\ub294 \ubaa8\ub4e0 \uc2e4\ud5d8 \uacfc\uc815\uc5d0\uc11c \uc0ac\uc6a9\ub418\ub294 \uc6a9\uc561\uc758 pH\uac00 \ubcc0\ud558\uac8c \ub418\uba74, \uc544\ubbf8\ub178\uc0b0\uc774\ub098 \ubc14\uc774\ub7ec\uc2a4 \ub4f1\uc758 \ub2e4\uc591\ud55c \uc2e4\ud5d8 \ub300\uc0c1\uccb4\uac00 \ud30c\uad34\ub418\uac70\ub098 \uae30\ub2a5\uc744 \uc783\uc5b4\ubc84\ub9ac\ub294 \ubb38\uc81c\uac00 \ubc1c\uc0dd\ud55c\ub2e4. \ub530\ub77c\uc11c \uc2e4\ud5d8 \uacfc\uc815\uc5d0\uc11c \ub2e4\uc591\ud55c \uc6a9\uc561\uc758 \ud63c\ud569\uc774 \ubc1c\uc0dd\ud558\ub354\ub77c\ub3c4 \ud5c8\uc6a9\ub418\ub294 \ubc94\uc704 \ub0b4\uc5d0\uc11c pH\ub97c \uc720\uc9c0\ud560 \uc218 \uc788\ub294 \uc7a5\uce58\uac00 \uc694\uad6c\ub41c\ub2e4. \uc774 \uacfc\uc815\uc5d0\uc11c \ud544\uc694\ud55c \uac83\uc774 \u2018\uc644\ucda9\uc6a9\uc561\u2019\uc774\ub2e4.<br \/>\n\u2018\uc644\ucda9\uc6a9\uc561\u2019\uc774\ub780 \uc0b0\uc774\ub098 \uc5fc\uae30\ub97c \ucd94\uac00\uc801\uc73c\ub85c \uac00\ud558\ub354\ub77c\ub3c4 pH \ubcc0\ud654\uc5d0 \uc800\ud56d\ud558\uba70, \uacf5\ud1b5\uc774\uc628\ud6a8\uacfc\uc5d0 \ub530\ub77c \uc6a9\uc561\uc758 pH\uac00 \ubcc0\ud654\uac00 \uc801\uc740 \uc6a9\uc561\uc744 \uc758\ubbf8\ud55c\ub2e4. \uc644\ucda9\uc6a9\uc561\uc740 HA\u21c4H++A- \uc758 \uac00\uc5ed\uc801\uc778 \ubc18\uc751\uc744 \ub530\ub974\uae30 \ub54c\ubb38\uc5d0, \uc57d\uc0b0\uc778 HA\ub294 OH+\ub97c \uc911\ud654\uc2dc\ud0a4\uace0, A-\ub294 \uc5fc\uae30\ub85c\uc11c \uc791\uc6a9\ud558\uc5ec H+\ub97c \uc911\ud654\uc2dc\ud0a8\ub2e4.<br \/>\n\uc644\ucda9\uc758 \uc815\ub3c4\ub294 \uc57d\uc0b0\uacfc \uadf8 \uc9dd\uc5fc\uae30\uc758 \uac00\uc5ed\uc801 \ubc18\uc751\uc758 \uc601\ud5a5\uc744 \ubc1b\ub294\ub2e4. \ub530\ub77c\uc11c \uc774\ub4e4\uc774 \uc6a9\uc561 \uc18d\uc5d0 \uc874\uc7ac\ud558\ub294 \ube44\uc728\uc5d0 \ub530\ub77c pH\uac00 \ubcc0\ud654\ud558\uac8c \ub41c\ub2e4. Henderson-Hasselbach \ubc29\uc815\uc2dd\uc5d0 \ub530\ub77c \uc57d\uc0b0\uacfc \uc9dd\uc5fc\uae30\uc758 \ube44\uc728\uc774 \uac19\uc740 [HA]=[A-]\uac00 \ub420 \ub54c pH=pKa\uac00 \ub418\ub294 \uc9c0\uc810\uc5d0\uc11c \uac00\uc7a5 \uc774\uc0c1\uc801\uc778 \uc644\ucda9 \ub2a5\ub825\uc744 \ub098\ud0c0\ub0b8\ub2e4.<br \/>\n(2) \ubab0\ub18d\ub3c4 \uac1c\ub150<br \/>\n\ubab0\ub18d\ub3c4 M(molarity)\ub294 1L\uc5d0 \ub4e4\uc5b4\uc788\ub294 \ubd84\uc790\uc758 \ubab0 \uc218\ub85c, M=mol\/L \uc774\ub2e4.<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/30033507\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 1. \uc2e4\ud5d8 \ubaa9\ud45c 2. Introduction 3. \uc2e4\ud5d8\ub3c4\uad6c 4. \uc2e4\ud5d8\ubc29\ubc95 5. \uacb0\uacfc: Buffer \uc81c\uc870\ud45c 1, Buffer \uc81c\uc870\ud45c 2 6. Discussion \ubcf8\ubb38\ub0b4\uc6a9 1. \uc2e4\ud5d8 \ubaa9\ud45c Column Chromatography\ub97c \ud558\uae30 \uc704\ud574 \ud544\uc694\ud55c Buffer\ub97c \uc81c\uc870\ud55c\ub2e4. Tris\uc640 HCl \ubc0f 3\ucc28 \uc99d\ub958\uc218\ub97c \uc774\uc6a9\ud558\uc5ec pH7.4\uc778 1M\uc758 Tris-HCl\uc744 \uc81c\uc870\ud558\ub294 \uc2e4\ud5d8\uc744 \uc9c4\ud589\ud558\uc600\ub2e4. 1M\uc758 Tris-HCl, MgSO4\uc640 NaCl\uc758 \ub18d\ub3c4\ub97c \ubc14\ud0d5\uc73c\ub85c \ub2e4\uc591\ud55c \ub18d\ub3c4\uc758 Elution buffer\uc758 \uad6c\uc131\uc131\ubd84\uc758 \uc6a9\ub7c9\uc744 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,425,46753,23352,53395],"class_list":["post-43029","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-12-","tag-46753","tag-23352","tag-53395"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43029","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=43029"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43029\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=43029"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=43029"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=43029"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}