{"id":43034,"date":"2026-06-10T09:00:43","date_gmt":"2026-06-10T00:00:43","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43034\/"},"modified":"2026-06-10T09:00:43","modified_gmt":"2026-06-10T00:00:43","slug":"%ec%83%9d%eb%aa%85%ea%b3%bc%ed%95%99%ec%8b%a4%ed%97%981-sds-page-%eb%a5%bc-%ec%9d%b4%ec%9a%a9%ed%95%9c-ap-%eb%8b%a8%eb%b0%b1%ec%a7%88-%eb%b6%84%ec%84%9d-%eb%b0%8f-coomassie-blue-staining","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43034\/","title":{"rendered":"\uc0dd\uba85\uacfc\ud559\uc2e4\ud5d81 SDS-PAGE \ub97c \uc774\uc6a9\ud55c AP \ub2e8\ubc31\uc9c8 \ubd84\uc11d \ubc0f Coomassie Blue Staining"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc8fc\uc81c
\n2. \uc2e4\ud5d8\uc694\uc57d
\n3. \uc6d0\ub9ac \ubc0f \ubc30\uacbd\uc9c0\uc2dd
\n4. \uc2e4\ud5d8 \uacb0\uacfc \ubc0f Discussion
\n5. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc8fc\uc81c
\nSDS-PAGE \ub97c \uc774\uc6a9\ud55c AP \ub2e8\ubc31\uc9c8 \ubd84\uc11d \ubc0f Coomassie Blue Staining
\n2. \uc2e4\ud5d8 \uc694\uc57d
\n(1) Gel cassette \uc870\ub9bd
\nResolving gel \uacfc stacking gel \uc744 \ubd80\uc5b4 \uc804\uccb4\uc801\uc778 gel \uc744 \ub9cc\ub4e4 \uc218 \uc788\ub294 \ud2c0\uc774 \ub418\ub294 gelcassette \ub97c \uba3c\uc800 \uc870\ub9bd\ud55c\ub2e4. Gel \uc548\uc5d0 \uba3c\uc800 \ub4f1\uc758 \ubd88\uc21c\ubb3c\uc774 \ub4e4\uc5b4\uac00\ub294 \uac83\uc744 \ubc29\uc9c0\ud558\uae30 \uc704\ud574 \uc5d0\ud0c4\uc62c\uc744 \uc774\uc6a9\ud558\uc5ec \uc720\ub9ac\ud310\uc758 \uba3c\uc9c0\ub97c \ub2e6\uc544\uc900\ub2e4. \uc720\ub9ac\ud310\uc744 \uc870\ub9bd\ud558\uace0 \uace0\ubb34 \uc704\uc5d0 \uace0\uc815\ud55c \ud6c4, \ubb3c\uc9c8\uc774 \uc0c8\uc9c0 \uc54a\ub294\uc9c0 D.W \ub97c \ub123\uc5b4 \ud655\uc778\ud55c\ub2e4. \ubb3c\uc9c8\uc774 \uc0c8\uc9c0 \uc54a\ub294\ub2e4\ub294 \uac83\uc744 \ud655\uc778\ud558\uba74, D.W \ub97c \ub530\ub77c \ubc84\ub9b0 \ud6c4\uc5d0 3M paper \uc744 \uc774\uc6a9\ud558\uc5ec \uc591 \uc720\ub9ac\ud310\uc758 \ubb3c\uae30\ub97c \uc81c\uac70\ud55c\ub2e4.
\n(2) Gel \uc81c\uc791
\nSDS-PAGE \ub97c \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 \ubd84\uc11d\ud558\uae30 \uc704\ud574\uc11c\ub294 \ub2e8\ubc31\uc9c8 \ubd84\uc11d\uc5d0 \ud544\uc694\ud55c \ub3c4\uad6c\uc778 gel \uc744 \uc81c\uc791\ud574\uc57c \ud55c\ub2e4. \ubd84\uc11d\ud558\uace0\uc790 \ud558\ub294 \ub2e8\ubc31\uc9c8\uc778 AP \ub2e8\ubc31\uc9c8\uc758 \ud06c\uae30\uac00 160-180kDa \uc774\ub77c\ub294 \uac83\uc744 \ubc14\ud0d5\uc73c\ub85c 8% gel \uc744 \uc81c\uc791\ud558\uc600\ub2e4.
\n\uc544\ub798 \ud45c\uc758 volume \uc744 \ubc14\ud0d5\uc73c\ub85c \uba3c\uc800 50ml cornical tube \uc5d0 resolving gel \uc744 \ub9cc\ub4e0\ub2e4.
\n\uadf8\ub9ac\uace0 \uc774 \uc6a9\uc561\uc744 voltexing \uc744 \ud558\uc5ec \uc798 \uc11e\uc5b4\uc900 \ud6c4, \uc6a9\uc561 \uc57d 4.7ml \ub97c gel cassette \uc5d0 loading \ud55c\ub2e4. \uc774\ub807\uac8c loading \ub41c resolving gel \uc774 \uc644\uc804\ud788 \uad73\uc744 \ub54c\uae4c\uc9c0 30-40 \ubd84 \uc815\ub3c4 \uae30\ub2e4\ub9b0\ub2e4.
\nResolving gel \uc744 loading \ud558\uba74\uc11c \uc0dd\uae34 \uae30\ud3ec\ub97c \uc815\ub9ac\ud558\uc5ec gel \uc744 \ud3c9\ud3c9\ud558\uac8c \ub9cc\ub4e4\uc5b4\uc8fc\uace0, gel \uc774 \ub9c8\ub974\ub294 \uac83\uc744 \ubc29\uc9c0\ud558\uae30 \uc704\ud574 resolving gel \uc704\uc5d0 isopropanol \uc57d 1ml \ub97c loading \ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc8fc\uc81c 2. \uc2e4\ud5d8\uc694\uc57d 3. \uc6d0\ub9ac \ubc0f \ubc30\uacbd\uc9c0\uc2dd 4. \uc2e4\ud5d8 \uacb0\uacfc \ubc0f Discussion 5. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. \uc8fc\uc81c SDS-PAGE \ub97c \uc774\uc6a9\ud55c AP \ub2e8\ubc31\uc9c8 \ubd84\uc11d \ubc0f Coomassie Blue Staining 2. \uc2e4\ud5d8 \uc694\uc57d (1) Gel cassette \uc870\ub9bd Resolving gel \uacfc stacking gel \uc744 \ubd80\uc5b4 \uc804\uccb4\uc801\uc778 gel \uc744 \ub9cc\ub4e4 \uc218 \uc788\ub294 \ud2c0\uc774 \ub418\ub294 gelcassette \ub97c \uba3c\uc800 \uc870\ub9bd\ud55c\ub2e4. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,425,46753,23352,53395],"class_list":["post-43034","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-12-","tag-46753","tag-23352","tag-53395"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43034","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=43034"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43034\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=43034"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=43034"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=43034"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}