{"id":43035,"date":"2026-06-10T09:00:44","date_gmt":"2026-06-10T00:00:44","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43035\/"},"modified":"2026-06-10T09:00:44","modified_gmt":"2026-06-10T00:00:44","slug":"%ec%83%9d%eb%aa%85%ea%b3%bc%ed%95%99%ec%8b%a4%ed%97%981-western-blot","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43035\/","title":{"rendered":"\uc0dd\uba85\uacfc\ud559\uc2e4\ud5d81 Western Blot"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

0. \uc2e4\ud5d8\ubaa9\ud45c
\n1. Introduction
\n2. Method
\n3. Discussion
\n4. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

0. \uc2e4\ud5d8\ubaa9\ud45c
\nWestern Blot\uc744 \ud1b5\ud574 \uc5ec\ub7ec \ub2e8\ubc31\uc9c8\ub4e4\uc774 \ud63c\ud569\ub418\uc5b4 \uc788\ub294 \ub2e8\ubc31\uc9c8 sample\uc5d0\uc11c \uc6d0\ud558\ub294 \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc758 \uc874\uc7ac \uc5ec\ubd80\ub97c \uac80\ucd9c\ud558\uace0 \uc774\ub97c \uc2dc\uac01\uc801\uc73c\ub85c \ud655\uc778\ud55c\ub2e4. SDS-PAGE\ub97c \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 \ud06c\uae30 \ubcc4\ub85c \ubd84\ub958\ud558\uace0, \ubd84\ub958\ub41c \ub2e8\ubc31\uc9c8\uc744 membrane\uc73c\ub85c \uc62e\uae34 \ud6c4\uc5d0 \ud56d\uc6d0-\ud56d\uccb4 \uc0c1\ubcf4\uc801 \uacb0\ud569\uc744 \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc758 \uc874\uc7ac \uc5ec\ubd80\ub97c \uc2dc\uac01\ud654\ud558\uc5ec \uacb0\uacfc\ub97c \ud655\uc778\ud55c\ub2e4.
\n1. Introduction
\nWestern Blot\uc740 gel \uc804\uae30\uc601\ub3d9\uc744 \ud1b5\ud574 \ub2e8\ubc31\uc9c8\uc744 \ubd84\ub9ac\ud558\uace0, \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc758 \uc874\uc7ac \uc5ec\ubd80\ub97c \ud655\uc778\ud558\ub294 \uc2e4\ud5d8\uc774\ub2e4.
\n(1) Electrophoretic Separation: SDS-PAGE
\nElectrophoretic Separation\ub294 protein solution\uc744 gel\uc5d0 loading\ud558\uc5ec \uc804\uadf9\uc744 \uac78\uc5b4\uc8fc\uc5c8\uc744 \ub54c, poretin\ub4e4\uc774 \ud06c\uae30\uc5d0 \ub530\ub77c \uc11c\ub85c \ub2e4\ub978 \uc18d\ub3c4\ub85c \uc774\ub3d9\ud558\ub294 \uc131\uc9c8\uc744 \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 \ubd84\ub9ac\ud558\ub294 \ub2e8\ubc31\uc9c8 \ubd84\uc11d \ubc29\ubc95\uc774\ub2e4. SDS-PAGE\ub294 SDS\ub77c\ub294 detergent\ub97c \uc0ac\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc758 \uad6c\uc870\ub97c \uc120\ud615\uc73c\ub85c \ub9cc\ub4e6\uc73c\ub85c\uc368 \ub2e8\ubc31\uc9c8\uc758 \uc804\ud558 \ubc00\ub3c4\ub97c \uc77c\uc815\ud558\uac8c \ub9cc\ub4e4\uace0, \uacb0\uad6d \ub2e8\ubc31\uc9c8\ub4e4\uc774 \ud06c\uae30\ub85c\ub9cc \ubd84\ub958\ub420 \uc218 \uc788\uac8c \ud55c \uc804\uae30\uc601\ub3d9 \uae30\ubc95\uc774\ub2e4. Stacking gel\uacfc Running gel\uc5d0 \uac01\uac01 \uc21c\uc11c\ub300\ub85c 80V\uc640 120V\ub97c \uac78\uc5b4\uc8fc\uba74, \ub2e8\ubc31\uc9c8\ub4e4\uc774 \ud06c\uae30\uc5d0 \ub530\ub978 \uc774\ub3d9 \uc18d\ub3c4\uc5d0 \ucc28\uc774\uac00 \uc0dd\uaca8 gel \ub0b4\ubd80\uc5d0 \ud06c\uae30\ubcc4\ub85c \ubd84\ub958\uac00 \ub41c\ub2e4. \ub2e8\ubc31\uc9c8\uc758 \ubd84\uc790\ub7c9\uc744 \ub098\ud0c0\ub0b8 marker\ub97c \ud1b5\ud574 \uac01 \ub2e8\ubc31\uc9c8\uc774 \uc5bc\ub9c8\ub9cc\ud07c\uc758 \ubd84\uc790\ub7c9\uc744 \uac00\uc9c0\ub294\uc9c0 \ud655\uc778\ud560 \uc218 \uc788\uc73c\uba70, \ubd84\uc790\ub7c9\uc5d0 \ud574\ub2f9\ud558\ub294 \ub2e8\ubc31\uc9c8\uc744 \uc5ed\uc73c\ub85c \ucc3e\uc544\ub0bc \uc218 \uc788\ub2e4.
\n(2) Transfer
\n\uc55e\uc11c SDS-PAGE\ub97c \uc774\uc6a9\ud574 \ud06c\uae30\ubcc4\ub85c \uad6c\ubd84\ud55c \ub2e8\ubc31\uc9c8\uc744 membrane\uc73c\ub85c \uc62e\uae30\ub294 \ub2e8\uacc4\uc774\ub2e4.
\n\u2460 \ub2e8\ubc31\uc9c8\uc774 \uc62e\uaca8\uc9c0\ub294 \uc6d0\ub9ac<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 0. \uc2e4\ud5d8\ubaa9\ud45c 1. Introduction 2. Method 3. Discussion 4. Reference \ubcf8\ubb38\ub0b4\uc6a9 0. \uc2e4\ud5d8\ubaa9\ud45c Western Blot\uc744 \ud1b5\ud574 \uc5ec\ub7ec \ub2e8\ubc31\uc9c8\ub4e4\uc774 \ud63c\ud569\ub418\uc5b4 \uc788\ub294 \ub2e8\ubc31\uc9c8 sample\uc5d0\uc11c \uc6d0\ud558\ub294 \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc758 \uc874\uc7ac \uc5ec\ubd80\ub97c \uac80\ucd9c\ud558\uace0 \uc774\ub97c \uc2dc\uac01\uc801\uc73c\ub85c \ud655\uc778\ud55c\ub2e4. SDS-PAGE\ub97c \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc744 \ud06c\uae30 \ubcc4\ub85c \ubd84\ub958\ud558\uace0, \ubd84\ub958\ub41c \ub2e8\ubc31\uc9c8\uc744 membrane\uc73c\ub85c \uc62e\uae34 \ud6c4\uc5d0 \ud56d\uc6d0-\ud56d\uccb4 \uc0c1\ubcf4\uc801 \uacb0\ud569\uc744 \uc774\uc6a9\ud558\uc5ec \ub2e8\ubc31\uc9c8\uc758 \uc874\uc7ac \uc5ec\ubd80\ub97c \uc2dc\uac01\ud654\ud558\uc5ec \uacb0\uacfc\ub97c \ud655\uc778\ud55c\ub2e4. 1. Introduction […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,425,46753,23352,53395],"class_list":["post-43035","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-12-","tag-46753","tag-23352","tag-53395"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43035","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=43035"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43035\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=43035"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=43035"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=43035"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}