{"id":43039,"date":"2026-06-10T09:00:50","date_gmt":"2026-06-10T00:00:50","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43039\/"},"modified":"2026-06-10T09:00:50","modified_gmt":"2026-06-10T00:00:50","slug":"%ec%83%9d%eb%aa%85%ea%b3%bc%ed%95%99%ec%8b%a4%ed%97%981-immunoprecipitation-ip","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/06\/10\/43039\/","title":{"rendered":"\uc0dd\uba85\uacfc\ud559\uc2e4\ud5d81 Immunoprecipitation (IP)"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Introduction
\n2. Materials
\n3. Methods
\n4. Result
\n5. Discussion
\n6. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

(1) Immunoprecipitation (\uba74\uc5ed\uce68\uac15\ubc95, IP)
\n\uba74\uc5ed\uce68\uac15\ubc95\uc774\ub780 \ud56d\uc6d0-\ud56d\uccb4 \ubc18\uc751\uc744 \uc774\uc6a9\ud558\uc5ec \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8\uc744 \ubd84\ub9ac\ud558\uace0\uc790 \ud558\ub294 \uc2e4\ud5d8\uc774\ub2e4.
\n\ub2e8\ubc31\uc9c8\uc740 \ud2b9\uc815\ud55c \ud56d\uccb4\uc5d0\ub9cc \uacb0\ud569\ud558\ub294 \ud56d\uccb4 \ud2b9\uc774\uc131\uc744 \uac00\uc9c0\uace0 \uc788\ub2e4. \ub530\ub77c\uc11c \uac80\ucd9c\ud558\uace0\uc790\ud558\ub294 target protein \ub610\ud55c \ud2b9\uc815\ud55c \uc885\ub958\uc758 \ud56d\uccb4\uc640\ub9cc \uacb0\ud569\ud558\uac8c \ub41c\ub2e4. \ud56d\uc6d0-\ud56d\uccb4 \ubcf5\ud569\uccb4\uac00 \ubc14\ub2e5\uc5d0 \uac00\ub77c\uc549\ub3c4\ub85d \ud558\uae30 \uc704\ud574\uc11c \uba3c\uc800 \ud56d\uccb4\uc640 Bead \ub97c \ubd99\uc774\uace0, bead \uc640 \uacb0\ud569\ud55c \ud56d\uccb4\uac00 \ub2e4\uc2dc \ub2e8\ubc31\uc9c8\uacfc \uacb0\ud569\ud558\uc5ec \ub2e8\ubc31\uc9c8-\ud56d\uccb4-Bead \ubcf5\ud569\uccb4\ub97c \ud615\uc131\ud558\uac8c \ub41c\ub2e4. \uc774 \ubcf5\ud569\uccb4\uac00
\n\uc6d0\uc2ec\ubd84\ub9ac\ub97c \ud1b5\ud574 \uce68\uac15\ud558\uac8c \ub418\uba74\uc11c \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8\uc744 \ubd84\ub9ac\ud560 \uc218 \uc788\ub294 \uac83\uc774\ub2e4.
\n\u2460 Antibody (\ud56d\uccb4)
\n\ud56d\uccb4\ub294 \ud56d\uc6d0\uacfc \ud2b9\uc774\uc801\uc73c\ub85c \uacb0\ud569\ud558\ub294 \ubb3c\uc9c8\uc774\ub2e4. \ud56d\uccb4\ub294 \uc911\uc2ec\uc5d0 \ub450 \uac00\ub2e5\uc758 heavy chain \uacfc \uc8fc\ubcc0\uc5d0 \uac01\uac01 \ud55c \uac00\ub2e5\uc758 light chain \uc744 \uac00\uc9c0\uace0 \uc788\ub2e4. \uc804\uccb4\uc801\uc73c\ub85c \ud56d\uc6d0\uacfc \uc9c1\uc811\uc801\uc73c\ub85c \uacb0\ud569\ud558\ub294 \ubd80\uc704\uc778 antigen binding site \uc640 \uc911\uc2ec \uae30\ub465\uc774 \ub418\ub294 Fc domain \uc73c\ub85c \ub098\ub20c \uc218 \uc788\ub294 \ud615\ud0dc\ub97c \uac00\uc9c4\ub2e4. \uc0dd\uccb4 \ub0b4\uc5d0\ub294 \ub2e4\uc591\ud55c \ud56d\uccb4\ub97c \uac00\uc9c0\ub294\ub370, \uc774\ubc88 Immunoprecipitation \uc2e4\ud5d8 \uacfc\uc815\uc5d0\uc11c \uc0ac\uc6a9\ub420 \ud56d\uccb4\ub294 Ig G \uc774\ub2e4.
\nIg G \ud56d\uccb4\ub294 \uac10\uc5fc \uc2dc\uc5d0 \uba74\uc5ed \ubc18\uc751 \uacfc\uc815\uc5d0\uc11c \uc8fc\ub85c \uc0ac\uc6a9\ub418\ub294 \ud56d\uccb4\uc774\uba70, \uba74\uc5ed \ubc18\uc751 \ucd08\uae30\uc5d0 \uc0dd\uc131\ub418\ub294 \ud56d\uccb4\uc778 Ig M \uc774 \uba74\uc5ed \ubc18\uc751 \uacfc\uc815\uc5d0\uc11c Ig G \ub85c \ubc14\ub00c\uc5b4\uc11c \uc0dd\uc0b0\ub41c\ub2e4.
\n\u2461 Protein Tagging
\nProtein Tagging \uc740 \uac80\ucd9c\ud558\uace0\uc790 \ud558\ub294 \ubaa9\ud45c \ub2e8\ubc31\uc9c8\uc5d0 \uc778\uc704\uc801\uc73c\ub85c \ud569\uc131\ud55c \uc544\ubbf8\ub178\uc0b0 \ubc30\uc5f4 \uc9d1\ub2e8\uc744 \ubd99\uc774\uace0, \uc774 \uc544\ubbf8\ub178\uc0b0\uc5d0 \uacb0\ud569\ud558\ub294 \ud56d\uccb4\ub97c loading \ud558\uc5ec \ubaa9\ud45c \ub2e8\ubc31\uc9c8\uc744 \uac80\ucd9c\ud558\uace0\uc790 \ud55c\ub2e4. \uc774\ub807\uac8c tagging \ub41c \ub2e8\ubc31\uc9c8\ub9cc\uc744 \uc138\ud3ec\uc5d0\uc11c \uc778\uc704\uc801\uc73c\ub85c \ubc30\uc591\uc2dc\ud0a4\uba74 \uc6a9\uc561 \ub0b4\uc758 \uc5ec\ub7ec \ub2e8\ubc31\uc9c8\ub4e4 \uc911 \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8\ub9cc\uc744 \uc5bb\uc744 \uc218 \uc788\ub2e4<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Introduction 2. Materials 3. Methods 4. Result 5. Discussion 6. Reference \ubcf8\ubb38\ub0b4\uc6a9 (1) Immunoprecipitation (\uba74\uc5ed\uce68\uac15\ubc95, IP) \uba74\uc5ed\uce68\uac15\ubc95\uc774\ub780 \ud56d\uc6d0-\ud56d\uccb4 \ubc18\uc751\uc744 \uc774\uc6a9\ud558\uc5ec \uc6d0\ud558\ub294 \ub2e8\ubc31\uc9c8\uc744 \ubd84\ub9ac\ud558\uace0\uc790 \ud558\ub294 \uc2e4\ud5d8\uc774\ub2e4. \ub2e8\ubc31\uc9c8\uc740 \ud2b9\uc815\ud55c \ud56d\uccb4\uc5d0\ub9cc \uacb0\ud569\ud558\ub294 \ud56d\uccb4 \ud2b9\uc774\uc131\uc744 \uac00\uc9c0\uace0 \uc788\ub2e4. \ub530\ub77c\uc11c \uac80\ucd9c\ud558\uace0\uc790\ud558\ub294 target protein \ub610\ud55c \ud2b9\uc815\ud55c \uc885\ub958\uc758 \ud56d\uccb4\uc640\ub9cc \uacb0\ud569\ud558\uac8c \ub41c\ub2e4. \ud56d\uc6d0-\ud56d\uccb4 \ubcf5\ud569\uccb4\uac00 \ubc14\ub2e5\uc5d0 \uac00\ub77c\uc549\ub3c4\ub85d \ud558\uae30 \uc704\ud574\uc11c \uba3c\uc800 \ud56d\uccb4\uc640 Bead \ub97c […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9619,425,46753,23352,53395],"class_list":["post-43039","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-10-","tag-12-","tag-46753","tag-23352","tag-53395"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43039","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=43039"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/43039\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=43039"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=43039"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=43039"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}