{"id":45500,"date":"2026-07-04T09:02:27","date_gmt":"2026-07-04T00:02:27","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/04\/45500\/"},"modified":"2026-07-04T09:02:27","modified_gmt":"2026-07-04T00:02:27","slug":"genotypingpcr-gel-electrophoresis-a-report","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/04\/45500\/","title":{"rendered":"Genotyping(PCR, Gel electrophoresis) A+ report"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Introduction
\n1)\tObjective
\n2)\tPrinciple \ubc0f Theory<\/p>\n

2.\tMethods<\/p>\n

3.\tResults<\/p>\n

4.\tDiscussion<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

-Objective
\n \uc2e4\ud5d8 \ub300\uc0c1 mouse\uc758 \uc720\uc804\uc801 \uc815\ubcf4\ub97c \ud655\uc778\ud558\uae30 \uc704\ud574 \uc218\ud589\ub41c \uc2e4\ud5d8\uc73c\ub85c, \ud574\ub2f9 mouse\uc758 \uc720\uc804\uc801 \ubc30\uacbd\uc744 \ud30c\uc545\ud558\ub294 \uac83\uc744 \ubaa9\uc801\uc73c\ub85c \uc9c4\ud589\ub418\uc5c8\ub2e4. \uc774\ub97c \uc704\ud574 \uc720\uc804\uccb4 DNA\ub97c \ucd94\ucd9c\ud558\uc5ec \ud2b9\uc815 \uc720\uc804\uc790\ub098 \uc720\uc804\uc801 \ub9c8\ucee4\ub97c \ubd84\uc11d\ud568\uc73c\ub85c\uc368 \uc2e4\ud5d8 \ub300\uc0c1 mouse\uc758 \uc720\uc804\uc801 \ud2b9\uc131\uc744 \ud655\uc778\ud558\uace0\uc790 \ud558\uc600\ub2e4. \uc774\ub7ec\ud55c \uc2e4\ud5d8 \uc811\uadfc\ubc95\uc740 \uc2e4\ud5d8 \ub3d9\ubb3c\uc758 \uc720\uc804\uc801 \ubc30\uacbd\uc744 \uc774\ud574\ud558\uace0 \uc2e4\ud5d8\uacb0\uacfc\ub97c \ud574\uc11d\ud558\ub294 \ub370\uc5d0 \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud55c\ub2e4.
\n\ub610\ud55c, \uc774 \uc2e4\ud5d8\uc740 \ud2b9\uc815 \uc720\uc804\uc790\ub098 \uc720\uc804\uc801 \ubcc0\uc774\ub97c \ubc1c\uacac\ud558\uace0\uc790 \ud560 \ub54c\ub3c4 \uc0ac\uc6a9\ud560 \uc218 \uc788\ub2e4. \ud2b9\uc815 \uc720\uc804\uc790\uc758 \uc874\uc7ac \uc5ec\ubd80\ub098 \ud2b9\uc815 \ubcc0\uc774\uc758 \uc720\ubb34\ub97c \ud655\uc778\ud558\uace0\uc790 \ud560 \ub54c, \ud574\ub2f9 \uc720\uc804\uc790\uc758 \uc720\uc804\uccb4 DNA\ub97c \ucd94\ucd9c\ud558\uace0 PCR\uc744 \ud1b5\ud574 \uc99d\ud3ed\ud558\uc5ec \ubd84\uc11d \uac00\ub2a5\ud558\ub2e4. \uc774\ub294 \uc720\uc804\uc801 \ubcc0\ud654\ub97c \uc5f0\uad6c\ud558\uac70\ub098 \uc9c8\ubcd1 \uad00\ub828 \uc720\uc804\uc790\ub97c \ud0d0\uc0c9\ud558\ub294 \ub4f1\uc758 \ubaa9\uc801\uc73c\ub85c \uc720\uc6a9\ud558\uac8c \ud65c\uc6a9\ub420 \uc218 \uc788\ub2e4.<\/p>\n

-Principle \ubc0f Theory
\n\uac00. Genotyping
\n Genotyping\uc740 \uc720\uc804\ud615\uc9c8\uc744 \ubd84\uc11d\ud558\ub294 \ubc29\uc2dd\uc774\ub2e4. \ub450 \uac00\uc9c0 \uc778\uc0c1\uc758 \uc2dc\ub8cc\ub85c\ubd80\ud130 \uc720\uc804\uccb4\uc758 DNA\ub97c \uc5bb\uace0, \uc774\ub4e4\uc774 \uc5fc\uae30\uc11c\uc5f4\uc774\ub098 \uc720\uc804\uccb4 \uad6c\uc870\uc0c1\uc73c\ub85c \uc5b4\ub5bb\uac8c \ub2e4\ub978\uac00\ub97c \ud655\uc778\ud558\uc5ec genotype\uc73c\ub85c \uad6c\ubcc4\ud558\uace0\uc790 \ud558\ub294 \uc2e4\ud5d8\ubc95\uc774\ub2e4.
\n Genotyping\uc758 3 steps\ub294 \uc77c\ubc18\uc801\uc73c\ub85c, 1.DNA\ucd94\ucd9c, 2.target DNA\uc99d\ud3ed, 3.Run agarose gel(Genotyping\ubd84\uc11d) \ub2e8\uacc4\ub85c \uc9c4\ud589\ub41c\ub2e4. DNA\ub97c sample\uc5d0\uc11c \ucd94\ucd9c\ud558\uace0, PCR\ub97c \uc774\uc6a9\ud558\uc5ec \ud2b9\uc815 DNA\ub97c amplification\uc2dc\ud0a8\ub2e4. \uadf8 \ud6c4, \uc8fc\ub85c SNP(Single Nucleotide Polymorphism)\ub97c \uc870\uc0ac\ud558\uc5ec genotyping \ubd84\uc11d\uc744 \ud55c\ub2e4.<\/p>\n

\ub098. Genomic DNA
\n Genomic DNA(gDNA)\ub294 \uae34 \uc774\uc911\uc0ac\uc2ac\ub85c\uc11c, \ud575 \ub0b4\uc5d0 \uc874\uc7ac\ud558\ub294 \uc720\uae30\uccb4 \ub0b4\uc758 chromosomal DNA\uc774\ub2e4. gDNA\ub294 A, T, G, C\uc758 \uc5fc\uae30\uc30d\ub4e4\uc774 \uc720\uc804\uc815\ubcf4\ub97c \uc800\uc7a5\ud558\uba70 \uc0ac\ub78c\uc758 \uacbd\uc6b0\uc5d0\ub294 32\uc5b5 \uac1c \uc774\uc0c1\uc758 \uc5fc\uae30\uc30d\uc744 \uac16\ub294\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Introduction 1) Objective 2) Principle \ubc0f Theory 2. Methods 3. Results 4. Discussion \ubcf8\ubb38\ub0b4\uc6a9 -Objective \uc2e4\ud5d8 \ub300\uc0c1 mouse\uc758 \uc720\uc804\uc801 \uc815\ubcf4\ub97c \ud655\uc778\ud558\uae30 \uc704\ud574 \uc218\ud589\ub41c \uc2e4\ud5d8\uc73c\ub85c, \ud574\ub2f9 mouse\uc758 \uc720\uc804\uc801 \ubc30\uacbd\uc744 \ud30c\uc545\ud558\ub294 \uac83\uc744 \ubaa9\uc801\uc73c\ub85c \uc9c4\ud589\ub418\uc5c8\ub2e4. \uc774\ub97c \uc704\ud574 \uc720\uc804\uccb4 DNA\ub97c \ucd94\ucd9c\ud558\uc5ec \ud2b9\uc815 \uc720\uc804\uc790\ub098 \uc720\uc804\uc801 \ub9c8\ucee4\ub97c \ubd84\uc11d\ud568\uc73c\ub85c\uc368 \uc2e4\ud5d8 \ub300\uc0c1 mouse\uc758 \uc720\uc804\uc801 \ud2b9\uc131\uc744 \ud655\uc778\ud558\uace0\uc790 \ud558\uc600\ub2e4. \uc774\ub7ec\ud55c \uc2e4\ud5d8 \uc811\uadfc\ubc95\uc740 \uc2e4\ud5d8 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[42517,56093,31458,16630,20278,56092],"class_list":["post-45500","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cpcr","tag-dna-extraction","tag-dna-","tag-gel-electrophoresis","tag-genomic-dna-prep","tag-genotyping"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/45500","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=45500"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/45500\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=45500"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=45500"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=45500"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}